33,449 results
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Plasmid#80102PurposeEncodes a set of wild-type Cas1+2 and a mutant set of Cas1+2DepositorPublicationExpressionBacterialMutationQ24H, P202Q, G241D, E276D, L297QAvailable sinceDec. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pBiex-1 Myosin VI HMM_SNAP
Plasmid#82716PurposeExpresses Myosin VI HMM with a C-terminal SNAP tag.DepositorInsertMyosin VI
Tags6xHis Tag, FLAG purification tag, and SNAP tagExpressionBacterial and InsectPromoterT7 PromoterAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCis mCherry-SS Himar1 A7
Plasmid#84687PurposeCan be used for the transformation of rickettsia, anaplasma and ehrlichia. Uses the Himar1 transposon system, with the A7 allele and both transposase and transposon contained in one plasmid.DepositorTypeEmpty backboneExpressionBacterialAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_R472A_H477A
Plasmid#83483PurposeBacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).DepositorInsertLbu_C2c2_R472A_H477A
UseCRISPRTagsHis6-MBPExpressionBacterialMutationR472A, H477AAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNCS-CyRFP1
Plasmid#84546PurposeBacterial expression of CyRFP1DepositorInsertCyRFP1
TagsHisExpressionBacterialAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSKA413
Plasmid#80381PurposeConstitutive CcaS and CcaR with gfpmut3 under the cpcG2Δ59 promoter.DepositorInsertsccaS (complement)
ccaR (complement)
gfpmut3
UseSynthetic Biology; OptogeneticsTagsFLAG tagExpressionBacterialPromoterccaR and cpcG2Δ59Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHLUK
Plasmid#64181Purposeharbours marker genes HIS3, LEU2, URA3 and LYS2DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAV115
Plasmid#63182PurposepRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV116
Plasmid#63183PurposepRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTriEx-mCherry-Zdk1-Rac1 Q61L
Plasmid#81059Purposemammalian expression of Zdk1 tagged Rac1 Q61LDepositorAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOV2 C450A
Plasmid#81031PurposeMammalian expression of the dark state of LOV2DepositorInsertLOV2
TagsmCherryExpressionBacterial, Insect, and Mamm…MutationC450AAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCOLA-T7-WspR:D70E
Plasmid#79164PurposeExpresses constitutively active WspR D70E under a T7 promoterDepositorAvailable sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pG1AK-mCherry
Plasmid#71737PurposeModular shuttle vector for Geobacillus and E. coliDepositorPublicationInsertmCherry
UseSynthetic Biology; Shuttle vectorExpressionBacterialPromoterpRplsWTAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pG1AK-PheB
Plasmid#71738PurposeModular shuttle vector for Geobacillus and E. coliDepositorPublicationInsertPheB
UseSynthetic Biology; Shuttle vectorExpressionBacterialPromoterpRplsWTAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAN1201
Plasmid#74695PurposeCircuit backbone plasmid (LacZa insert)DepositorTypeEmpty backboneExpressionBacterialAvailable sinceJune 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSpal2At
Plasmid#78286PurposeExpresses A. thaliana PAL2 in E. coliDepositorAvailable sinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAN4037
Plasmid#74703PurposeOutput plasmid: PPhlF-PBetI-YFPDepositorInsertPPhlF-PBetI-YFP
ExpressionBacterialAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
NUDT1
Plasmid#74660PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable sinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZ8-Prp
Plasmid#74065PurposepZ8-1 with the PprpD2 propionate-inducible promoter instead of Ptac, KanRDepositorPublicationInsertPropionate inducible promoter
UseSynthetic BiologyExpressionBacterialPromoterprpAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK642
Plasmid#72391PurposeProduces Escherichia coli glutamine synthetase, N-terminal His6 tag and Y397F mutation that prevents adenylation (H6EcGlnA-Y397F)DepositorInsertglutamine synthetase (glnA Escherichia coli str. K-12 substr. MG1655))
TagsHis6ExpressionBacterialMutationMutated tyrosine-397 to phenylalanine to block ad…PromoterT7Available sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by