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pC0067 PguCas13 (B11) His6-TwinStrep-SUMO-BsaI

Plasmid

#115218

Purpose

Expresses PguCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Publication

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

PguCas13

Tags

SUMO

Expression

Bacterial

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI

Plasmid

#115216

Purpose

Expresses Pin2Cas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Publication

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

Pin2Cas13

Tags

SUMO

Expression

Bacterial

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pQE80L TriCatcher-ELP

Plasmid

#112630

Purpose

Two SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcher

Depositor

Mark Howarth

Publication

Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

Insert

TriCatcher-ELP

Tags

His6 and TEV cleavage site

Expression

Bacterial

Promoter

T5

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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AFDN_PDZ_1

Plasmid

#103938

Purpose

Protein expression and purification of TRITHORAX PDZ domain

Depositor

Sachdev Sidhu

Publication

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

AFDN (AFDN Human)

Tags

GST

Expression

Bacterial

Mutation

contains AA983-1110 of AFDN

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAJM.969

Plasmid

#108537

Purpose

MphRAM + EryR + PMph-YFP Reporter. Ery sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

MphRAM + EryR + PMph-YFP

Expression

Bacterial

Mutation

MphR: N94D

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.716

Plasmid

#108518

Purpose

PBAD-YFP Reporter. Ara sensor for use in Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PBAD-YFP

Expression

Bacterial

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pBTK570

Plasmid

#110615

Purpose

BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by PA3 promoter

Depositor

Jeffrey Barrick

Publication

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

E2-Crimson

Expression

Bacterial

Available since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pSW002-Pc-E2-Crimson

Plasmid

#111252

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with E2-Crimson

Depositor

Rosemarie Wilton

Publication

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

E2-Crimson

Expression

Bacterial

Promoter

Pc

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET-2(KW)2-mCherry

Plasmid

#112738

Purpose

Express 2(KW)2-mCherry in E. coli

Depositor

Kyubong Jo

Publication

Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

Insert

2(KW)2-mCherry

Tags

6xHis

Expression

Bacterial

Promoter

T7 Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pMBP-LbCas12a H759A

Plasmid

#113518

Purpose

Expresses His-MBP fused to LbCas12a with H759A mutation to inactivate crRNA processing

Depositor

Jennifer Doudna

Publication

Chen et al Science. 2018 Apr 27;360(6387):436-439.

Insert

LbCas12a

Tags

MBP

Expression

Bacterial

Mutation

H759A

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pET-2(KW)2-eGFP

Plasmid

#112736

Purpose

Express 2(KW)2-eGFP in E. coli

Depositor

Kyubong Jo

Publication

Lee et al Nucleic Acids Res. 2016 Jan 8;44(1):e6.

Insert

2(KW)2-eGFP

Tags

6xHis

Expression

Bacterial

Promoter

T7 Promoter

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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His6-Twin-Strep-SUMO-pET28a-EfaCas2

Plasmid

#112794

Purpose

To express enterococcus faecalis Cas2 protein in E. coli

Depositor

Ailong Ke

Publication

Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

Insert

EfaCas2

Tags

His6-Twin-Strep-SUMO

Expression

Bacterial

Available since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSW003

Plasmid

#111249

Purpose

Broad host-range bacterial vector

Depositor

Rosemarie Wilton

Publication

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Type

Empty backbone

Expression

Bacterial

Available since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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pBb(B5)8k-sfGFP

Plasmid

#110140

Purpose

IPTG-inducible pTRC promoter expressing sfGFP on BBR1-B5 origin

Depositor

Brian Pfleger

Publication

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

sfGFP

Expression

Bacterial

Available since

July 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.712

Plasmid

#108513

Purpose

PCymRC-YFP Reporter. Cuma sensor for use in Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PCymRC-YFP

Expression

Bacterial

Available since

June 30, 2018

Availability

Academic Institutions and Nonprofits only

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pET24a-VHH-tev-std

Plasmid

#109423

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody with a TEV site. VHH-tev-std is flanked by an N-terminal T7 epitope, and a C-terminal cassette composed of HA, BAP and a His6 epitope

Depositor

Martin Spiess

Publication

Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

anti-GFP nanobody fused to a T7, TEV cleavage site, HA, BAP and His6 epitope

Tags

BAP, HA, His6, and T7

Expression

Bacterial

Promoter

T7

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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miniTn7(Gm)PA1/04/03-eyfp-a

Plasmid

#111620

Purpose

eYFP Fluorescent tagging of bacteria at their Tn7-site

Depositor

Tim Tolker-Nielsen

Publication

Klausen et al Mol Microbiol. 2003 Jun;48(6):1511-24.

Insert

PA1/04/03-eyfp

Expression

Bacterial

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMEXC3GH (Kan)

Plasmid

#110081

Purpose

Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Publication

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMEXC3GH (Hyg)

Plasmid

#110082

Purpose

Analogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Publication

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - GFP - His10 Tag

Expression

Bacterial

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLys-M1

Plasmid

#109382

Purpose

Arabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coli

Depositor

Tom Ellis

Publication

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-VioB-mCherry

Expression

Bacterial

Available since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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