30,806 results

1. pHAGE UbiC NLS-HA-stdMCP-stdHalo-RH1

   Plasmid

   #164614

   Purpose

   Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag and the RH1 domain of RILPL2. Used for single-molecule imaging of actin-anchored mRNA.

   Depositor

   Jeffrey Chao

   Article

   Mateju et al Cell. 2020 Dec 23;183(7):1801-1812.e13.

   Insert

   stdMCP-stdHalo-RH1
   

   Use

   Lentiviral

   Tags

   HA tag and NLS

   Expression

   Bacterial and Mammalian

   Promoter

   UbiC

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pReporter_4

   Plasmid

   #60565

   Purpose

   A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Reporter_4
   

   Expression

   Bacterial

   Promoter

   BBa_J23119

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHH0103_HCK-1/1

   Plasmid

   #91273

   Purpose

   Protein expression and purification of human SH3 domain construct HCK-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   HCK-1/1 (HCK Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDW363

   Plasmid

   #8842

   Depositor

   David Waugh

   Article

   Tsao et al Gene. 1996 Feb 22. 169(1):59-64.

   Type

   Empty backbone

   Tags

   MBP and biotin acceptor peptide

   Expression

   Bacterial

   Available Since

   April 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNIC- AVITAG-CTH10STII- -GG

   Plasmid

   #215805

   Purpose

   Cloning of target gene with C terminal non cleavable AVITAG and TEV cleavage of C terminal His10 and Twin Strep tag

   Depositor

   ASAP Discovery, Frank von Delft

   Article

   Fairhead et al bioRxiv 2024.02.13.579886

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pET-6xHis-mCherry(-6)

   Plasmid

   #205045

   Purpose

   Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   mCherry(-6)
   

   Tags

   MGHHHHHGG

   Expression

   Bacterial

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pnCS_SEPT7_SEPT9_i3-TEV-Strep

   Plasmid

   #174501

   Purpose

   bacterial co-expression of human SEPT7 and of human SEPT9_i3

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Inserts

   SEPTIN7 (SEPTIN7 Human)
   SEPTIN9 (SEPTIN9 Human)
   

   Tags

   TEV-Strep

   Expression

   Bacterial

   Available Since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJ151-HDR

   Plasmid

   #113631

   Purpose

   Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.

   Depositor

   Alex Kentsis

   Inserts

   Left homology arm
   Puromycin resistance gene
   Venus fluorescence gene
   Thymidine kinase
   Right homology arm
   

   Use

   Cre/Lox; Homology recombination selection vector

   Expression

   Bacterial and Mammalian

   Promoter

   EF1a promoter, EF1a promoter (T2A cleaving sequen…

   Available Since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLIC-EcTOP

   Plasmid

   #224463

   Purpose

   Expression of recombinant Escherichia coli topoisomerase I

   Depositor

   Yuk-Ching Tse-Dinh

   Article

   Tan et al Nucleic Acids Res. 2015 Dec 15;43(22):11

   Insert

   Escherichia coli TopA
   

   Tags

   6xHis tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. UbcH5B

    Plasmid

    #60443

    Purpose

    Express E2 UbcH5B in E. Coli

    Depositor

    Cynthia Wolberger

    Article

    Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

    Insert

    UbcH5B (UBE2D2 Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pSEVA228-pro4IUPi

    Plasmid

    #122018

    Purpose

    Expresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.

    Depositor

    Gregory Stephanopoulos

    Article

    Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

    Inserts

    Choline kinase (CKI1 Budding Yeast)
    Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
    isopentenyl-diphosphate Delta-isomerase
    

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pro4

    Available Since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBbE1a-RFP

    Plasmid

    #35331

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Trc

    Available Since

    Feb. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBlueKan+cysMPro

    Plasmid

    #187879

    Purpose

    Escherichia coli plasmid containing unique cloning sites behind a Campylobacter jejuni cysM promoter. Cloned genes will be expressed from the constitutively expressed C. jejuni promoter.

    Depositor

    Gaylen Uhlich

    Article

    Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    CysM

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21b-RBD-JA1

    Plasmid

    #167126

    Purpose

    Expresses SARS-CoV-2 RBD in bacterial system

    Depositor

    David Margulies

    Article

    Ahmad et al bioRxiv. 2021 Jan 27. doi: 10.1101/2021.

    Insert

    SARS-CoV-2 Receptor Binding domain (S SARS-CoV-2)
    

    Expression

    Bacterial

    Mutation

    residues (333 to 529 aa)

    Promoter

    T7

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19-link-mCherry-FLAG:cat

    Plasmid

    #211831

    Purpose

    Plasmid template that encodes the fluorescent protein mCherry with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out mcherry-FLAG-cat for genetic tagging.

    Depositor

    Peng Chen

    Insert

    mCherry-FLAG-cat
    

    Expression

    Bacterial

    Mutation

    NA

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC18/promSau/Lysostaphin

    Plasmid

    #125766

    Purpose

    Production of staphylolytic protein lysostaphin from a constitutive promoter

    Depositor

    Benedykt Wladyka

    Article

    Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

    Insert

    lysostaphin
    

    Expression

    Bacterial

    Mutation

    sequence corresponding to the mature form protein

    Promoter

    pemIK constitutive promoter

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-6xHis-(pos16)GFP

    Plasmid

    #89249

    Purpose

    Expresses GFP with +16 theoretical charge in E.Coli

    Depositor

    David Liu

    Article

    Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

    Insert

    His6-(pos16)-GFP
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET15b SARS-CoV PLpro

    Plasmid

    #61693

    Purpose

    inducible expression of SARS PLpro protease

    Depositor

    Michael Johnson

    Article

    Lee et al ChemMedChem. 2013 Aug;8(8):1361-72. doi:

    Insert

    SARS-CoV PLpro
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pQE80L SpyTag-ELP-SpyTag

    Plasmid

    #112634

    Purpose

    Two SpyTags linked by an elastin-like polypeptide for hydrogel formation

    Depositor

    Mark Howarth

    Article

    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

    Insert

    SpyTag-ELP-SpyTag
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    central cysteine

    Promoter

    T5

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTW084b

    Plasmid

    #116894

    Purpose

    Activity-independent SE-PACE AP

    Depositor

    David Liu

    Article

    Wang et al Nat Chem Biol. 2018 Aug 20. pii: 10.1038

    Inserts

    g3N
    434-cI/scFv m3
    

    Expression

    Bacterial

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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