30,807 results

1. pEB1-mCerulean

   Plasmid

   #103968

   Purpose

   Plasmid encoding mCerulean

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   mCerulean
   

   Use

   Low copy

   Expression

   Bacterial

   Mutation

   Mutations relative to wild-type GFP (F64L, S65T, …

   Promoter

   ProC

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. Clomeleon

   Plasmid

   #203723

   Purpose

   A genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentration

   Depositor

   George Augustine

   Article

   Kuner et al Neuron. 2000 Sep;27(3):447-59. doi: 10.1

   Insert

   cyan fluorescent protein linked yellow florescent protein
   

   Expression

   Bacterial and Mammalian

   Mutation

   Cyan Fluorescent Protein, Topaz Fluorescent Prote…

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28sumo-MDA5-Full-length

   Plasmid

   #210826

   Purpose

   protein expression in ecoli

   Depositor

   Anna Pyle

   Article

   Fitzgerald et al Nucleic Acids Res. 2014 Apr;42(6):3919-3

   Insert

   MDA5 (IFIH1) Full Length Protein (IFIH1 Human)
   

   Expression

   Bacterial

   Mutation

   NA

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMis1_1B

   Plasmid

   #192079

   Purpose

   A pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent induction

   Depositor

   Markus Buchhaupt

   Article

   Poschel et al Microbiologyopen. 2022 Oct;11(5):e1325.

   Inserts

   KanR
   rhaR
   rhaS
   pBBR1 rep
   

   Expression

   Bacterial

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSP574

   Plasmid

   #98807

   Purpose

   Expression Vector

   Depositor

   David Liu

   Article

   Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

   Insert

   pET28 MBP-ENLYFQS-GST
   

   Expression

   Bacterial

   Mutation

   None

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSANG14-3F

   Plasmid

   #39265

   Depositor

   John McCafferty

   Article

   Martin et al BMC Biotechnol. 2006 Dec 7;6:46.

   Type

   Empty backbone

   Tags

   6xHis, Alkaline phosphatase, pelB leader, and tri…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-4T-1 3xFlag-CREB

   Plasmid

   #82721

   Purpose

   Expresses human CREB

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   CREB (CREB1 Human)
   

   Tags

   3XFLAG and GST

   Expression

   Bacterial

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHis17_ParM _1

   Plasmid

   #78201

   Purpose

   Expression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensor

   Depositor

   Martin Webb

   Article

   Kunzelmann et al J Biol Chem. 2009 Nov 27;284(48):33130-8

   Insert

   parM (K33A I27C T174A T175N C287A)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   K33A I27C T174A T175N C287A

   Promoter

   unknown

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST15 hSlp4-a

   Plasmid

   #40046

   Depositor

   Fernando Martin-Belmonte

   Article

   Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

   Insert

   hSlp4-a (SYTL4 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pMA7-SacB

    Plasmid

    #79968

    Purpose

    TM-MAGE strain

    Depositor

    Morten Sommer

    Article

    Lennen et al Nucleic Acids Res. 2016 Feb 29;44(4):e36

    Inserts

    Lambda Red recombinase beta subunit
    DNA adenine methylase
    Levansucrase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pM1s3ATScsg-Etag

    Plasmid

    #137946

    Purpose

    E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and temperature sensitive curli expression

    Depositor

    Neel Joshi

    Article

    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102

    Inserts

    Synthetic curli operon
    Synthetic curli operon
    

    Use

    Synthetic Biology; For use with e. coli nissle in…

    Expression

    Bacterial

    Promoter

    pTlpA36

    Available Since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBAD-luxOFP

    Plasmid

    #229945

    Purpose

    Bacterial expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)

    Depositor

    Andy Yeh

    Article

    Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

    Insert

    luxOFP
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDEST17-UbE2D3

    Plasmid

    #15784

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2D3 (UBE2D3 Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    see Depositor Comments below

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET_RGG

    Plasmid

    #124929

    Purpose

    Expression of RGG domain from LAF-1 gene

    Depositor

    Matthew Good, Daniel Hammer, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    LAF-1 RGG domain (laf-1 Nematode)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    RGG domain

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBSK

    Plasmid

    #67504

    Purpose

    Express the tRNALys scaffold

    Depositor

    Luc Ponchon

    Article

    Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

    Insert

    modified tRNALys human
    

    Expression

    Bacterial

    Promoter

    lpp

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pRSET-iGluSnFR4s

    Plasmid

    #234443

    Purpose

    Bacterial expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow)

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s
    

    Tags

    6xHis-Xpress epi

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYX-C-mScarlet-Zeo-TSC22D2

    Plasmid

    #222670

    Purpose

    endogenously tag TSC22D2 with C-terminal mScarlet

    Depositor

    Jason Moffat

    Article

    Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

    Insert

    TSC22D2 (TSC22D2 Human)
    

    Tags

    mScarlet

    Expression

    Bacterial

    Available Since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. mKO2-pBAD

    Plasmid

    #54555

    Purpose

    Localization: Protein Expression Vector , Excitation: 551, Emission: 565

    Depositor

    Michael Davidson, Atsushi Miyawaki

    Article

    Sakaue-Sawano et al Cell. 2008 Feb 8;132(3):487-98. doi: 10.

    Type

    Empty backbone

    Tags

    6xHis and mKO2

    Expression

    Bacterial

    Available Since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS human c-Myc

    Plasmid

    #14971

    Depositor

    Joan Massague

    Article

    Siegel et al J Biol Chem. 2003 Sep 12. 278(37):35444-

    Insert

    c-Myc (MYC Human)
    

    Expression

    Bacterial

    Available Since

    May 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMW37: pRS426-R2Bm RNA

    Plasmid

    #209291

    Purpose

    Contains the R2Bm non-LTR retrotransposon 5'UTR and 3'UTR sequences

    Depositor

    Feng Zhang

    Article

    Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

    Insert

    R2Bm 5'UTR and 3'UTR
    

    Expression

    Bacterial and Yeast

    Promoter

    GAL1 GAPDH

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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