29,913 results

1. pGEX-4T HHARI RBR

   Plasmid

   #31254

   Depositor

   Rachel Klevit

   Article

   Wenzel et al Nature. 2011 May 1. ():.

   Insert

   Human Homologue of Ariadne (HHARI) (ARIH1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   RBR domain residues 177-395

   Promoter

   Available since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pMAL-c5X-ELP[V-60]

   Plasmid

   #67013

   Purpose

   Expresses malE-ELP[V-60] in E. coli

   Depositor

   Ashutosh Chilkoti

   Article

   Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

   Insert

   malE-ELP[V-60]
   

   Use

   Tags

   His6 and malE-Factor Xa

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRE-Tn7-140

   Plasmid

   #118556

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   TagBFP2
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET empty polycistronic destination vector (2D)

   Plasmid

   #29774

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCC1-4k-BBclone2

   Plasmid

   #137070

   Purpose

   E. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Inserts

   BB0323
   P13
   DipA
   Lmp1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   wild-type, full-length (signal-peptide-containing…

   Promoter

   Available since

   May 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. p404TDH3

   Plasmid

   #15973

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   TDH3 promoter + polylinker + CYC1 terminator (TDH3 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   TDH3 promoter is between SacI and XbaI restrictio…

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-15b(+) Naegleria fowleri PRL (Δprenylation motif)

   Plasmid

   #166421

   Purpose

   Express pET-15b(+) Naegleria fowleri PRL (Δprenylation motif)

   Depositor

   Kalle Gehring

   Article

   Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

   Insert

   Naegleria fowleri PRL
   

   Use

   Tags

   N-terminal His-tag

   Expression

   Bacterial

   Mutation

   Deleted nucleotides coding for 4 C-terminal amino…

   Promoter

   AmpR, lac1

   Available since

   Sept. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. 6xHis(TEV)-Drp1(K38A)-StrepII

   Plasmid

   #174423

   Purpose

   Expresses human Drp1 Isoform 3 with K38A mutation in bacteria

   Depositor

   Thomas Pucadyil

   Article

   Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

   Insert

   Drp1
   

   Use

   Tags

   6xHis(TEV) and StrepII

   Expression

   Bacterial

   Mutation

   K38A

   Promoter

   T7

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pScaf-5544.4

   Plasmid

   #111524

   Purpose

   pScaf phagemid with insert for generating DNA origami scaffold 5544 bases in length (v4)

   Depositor

   Shawn Douglas

   Article

   Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

   Insert

   pScaf-KH'
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQFmcs-2H12.D11-scarREF

    Plasmid

    #221184

    Purpose

    cdGreen2 expressed from a cumate-inducible promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11240)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Inserts

    cdGreen2
    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminus changed: starts with MSKKYGEAVIKE ...

    Promoter

    None (same as cdGreen2) and PQ5 (cumate-inducible)

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR221-SAR1-no stop

    Plasmid

    #67345

    Purpose

    hSar1 without stop codon in Gateway Entry vector

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    SAR1 (SAR1A Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    None

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pBS aP2 promoter (5.4kb) polyA

    Plasmid

    #11424

    Depositor

    Ronald Kahn

    Insert

    aP2 promoter (Fabp4 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAH01Sp::Cas9D10A

    Plasmid

    #128161

    Purpose

    Ptet-controlled expression of S. pyogenes Cas9D10A variant

    Depositor

    Calvin Henard

    Article

    Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

    Insert

    Sp Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A

    Promoter

    Ptet

    Available since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRAF-MutL

    Plasmid

    #86607

    Purpose

    E. coli MutL expression plasmid

    Depositor

    Richard Fishel

    Article

    Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

    Insert

    E. coli MutL
    

    Use

    Tags

    his-tag and ald-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTlpA-Wasabi-NF

    Plasmid

    #86100

    Purpose

    Nonfluorescent variant of mWasabi reporter gene gated by wild type TlpA

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TlpA
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D341A and S71T, G73A

    Promoter

    TlpA and TlpA, LacI

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMUT2-VtrA-mCherry

    Plasmid

    #192862

    Purpose

    pMUT2 based vector for sensing human secondary bile acids in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    VtrA-CadC fusion protein and cognate promoter inducible by secondary bile acids
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET11a+CYP119

    Plasmid

    #66131

    Purpose

    Expresses the wild type of CYP119

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. MP2

    Plasmid

    #69628

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E. coli PBAD

    Available since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. HLA-A2.1

    Plasmid

    #14904

    Depositor

    Linda Sherman

    Article

    Irwin et al J Exp Med. 1989 Oct 1. 170(4):1091-101.

    Insert

    HLA-A2.1 (HLA-A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKB231

    Plasmid

    #170018

    Purpose

    Encodes LacI-controlled expression of surface-displayed cecropin P1 and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    Cecropin P1
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and PvirK

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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