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34,021 results

Showing: 5241 - 5260 of 34021 results

  1. pHN767

    Plasmid
    #178884
    Purpose
    Expression of bacterial PNGase
    Depositor
    Kazuhiro Abe
    Insert
    Peptide:N-glycosidase F
    Tags
    GST, His8
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Feb. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    178884-plasmid-map-sequence-id-354970

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  2. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

    Plasmid
    #181745
    Purpose
    dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1
    Depositor
    Benjamin Kleinstiver
    Publication
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    human/zebrafish codon optimized SpCas9
    Use
    In vitro transcription; t7 promoter
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    181745-plasmid-map-sequence-id-354676

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  3. pTU422

    Plasmid
    #178982
    Purpose
    13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)
    Depositor
    Stan Brouns
    Publication
    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353
    Inserts
    gRAMP
    CRISPR array
    Tags
    Strep-Tag II SUMO
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Feb. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    178982-plasmid-map-sequence-id-354261

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  7. pRedCmOcr

    Plasmid
    #176226
    Purpose
    A helper plasmid for expression of the artificial T7ocr-gam-bet-exo operon. The plasmid carries the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage.
    Depositor
    Sergey Sineoky
    Publication
    Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89
    Insert
    Lambda Red-T7ocr operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PrhaB and PL
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176226-plasmid-map-sequence-id-347002

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  9. pRelA'

    Plasmid
    #175595
    Purpose
    Tet inducible, KAN Resistant, non labelled synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.
    Depositor
    Greg Bokinsky
    Publication
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    Catalytic domain of E.coli relA gene.
    Expression
    Bacterial
    Mutation
    Only the catalytic domain of the enzyme is used (…
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    175595-plasmid-map-sequence-id-348284

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  10. pCK01_PalkS_alkS

    Plasmid
    #166502
    Purpose
    Expression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.
    Depositor
    Sven Panke
    Publication
    Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2
    Insert
    alkS
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    L401G
    Promoter
    PalkS
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    166502-plasmid-map-sequence-id-333027

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  15. pAH69

    Plasmid
    #173782
    Purpose
    The pAH69 plasmid [Haldimann and Wanner 2001] is the HK022 helper plasmid necessary for genomic integration of the pIT5_KH plasmid.
    Depositor
    Keith Shearwin
    Publication
    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102
    Insert
    HK022 Integrase
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    Sept. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    173782-plasmid-map-sequence-id-342364

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Showing: 5241 - 5260 of 34021 results