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34,232 results

Showing: 5441 - 5460 of 34232 results

  7. pNR64

    Plasmid
    #79540
    Purpose
    Input plasmid: expresses two recombinases downstream of two inducible promoters. Same as Dual-Recombinase-Controller (plasmid #44456) from Endy Lab except with KanR instead of CamR on the backbone.
    Depositor
    Timothy Lu
    Publication
    Roquet et al Science. 2016 Jul 22;353(6297):aad8559.
    Inserts
    TP901
    BxbI
    Tags
    His tag and LAA degradation tag
    Expression
    Bacterial
    Available since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79540-plasmid-map-sequence-id-148741

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  9. PTP1B-1-401

    Plasmid
    #78683
    Purpose
    bacterial expression of PTP1B aa 1-401
    Depositor
    Eli Chapman
    Insert
    PTPN1 (PTPN1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    delta 402-435
    Promoter
    araBAD
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    78683-plasmid-map-sequence-id-152426

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  10. pSLC-238

    Plasmid
    #73192
    Purpose
    Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (chpB) under the control of rhamnose induceable promoter (PrhaB) for negative selection.
    Depositor
    Swaine Chen
    Publication
    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8
    Insert
    PrhaB-chpB (chpB )
    Expression
    Bacterial
    Promoter
    PrhaB
    Available since
    June 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    73192-plasmid-map-sequence-id-151373

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  14. KDM4D

    Plasmid
    #73247
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KDM4D (KDM4D Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam02375:JmjN 18-64, pfam02373:JmjC 182-295
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73247-plasmid-map-sequence-id-137202

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  17. pMD19T-psba1-TetR-PL22-dCas9-SpR

    Plasmid
    #73223
    Purpose
    Contains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.
    Depositor
    Paul Hudson
    Publication
    Yao et al ACS Synth Biol. 2015 Dec 28.
    Insert
    dCas9 from S. pyogenes
    Tags
    c-myc
    Expression
    Bacterial
    Mutation
    Silent mutation at bp 1341 A->C to remove an E…
    Promoter
    PL22
    Available since
    March 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73223-plasmid-map-sequence-id-135055

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  18. pdCas9-M-G6

    Plasmid
    #73437
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.
    Depositor
    Mattheos Koffas
    Publication
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor G6 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    73437-plasmid-map-sequence-id-135336

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  19. pKS011

    Plasmid
    #65464
    Purpose
    pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) MBP (Maltose Binding Protein), Ntag, and mCherry
    Depositor
    Keith Tyo
    Publication
    Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do
    Insert
    MBP-Ntag-mCherry
    Tags
    FLAG and MBP
    Expression
    Bacterial
    Promoter
    pl-TetO
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    65464-plasmid-map-sequence-id-116873

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Showing: 5441 - 5460 of 34232 results