30,262 results

1. pTrcHisB-TRF1

   Plasmid

   #53209

   Purpose

   Expressed N-terminally Hexahistidine tagged TRF1 protein

   Depositor

   Jack Griffith

   Article

   Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

   Insert

   TRF1 (TERF1 Human)
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   3-439

   Promoter

   Trc

   Available since

   May 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTrc99A-Rat FABP1

   Plasmid

   #13577

   Depositor

   Martin Scanlon

   Article

   Velkov et al Protein Expr Purif. 2005 Nov . 44(1):23-

   Insert

   Rat liver fatty acid binding protein (Fabp1 Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 15, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pROP-PP-FBFP

   Plasmid

   #122137

   Purpose

   FMN-based fluorescent protein Reporter plasmid for B-Rex contain perfect operator sequence (anaerobic purpose)

   Depositor

   Srivatsan Raman

   Article

   Liu et al ACS Synth Biol. 2019 Feb 15;8(2):264-273

   Insert

   FBFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23101

   Available since

   March 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. "AND-gate" receiver plasmid

   Plasmid

   #127676

   Purpose

   Encodes the transcriptional activator LuxR and GFP. LuxR has an pLlacO-1 promoter and the GFP has a pLux promoter.

   Depositor

   Friedrich Simmel

   Article

   Schwarz-Schilling et al Integr Biol (Camb). 2016 Apr 18;8(4):564

   Insert

   LuxR, GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLlacO-1 for LuxR, pLux for GFP

   Available since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQCasTns(Ptr)-array 8

   Plasmid

   #175580

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Article

   Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

   Insert

   PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(8 CRISPR array)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   NO

   Promoter

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRE-Tn5-155

   Plasmid

   #118537

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mScarlet-I
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCVL.SSA TLR (Sce)

   Plasmid

   #45576

   Purpose

   Codes for the SSA-TLR with the target site for I-Sce I in a lentiviral backbone

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Inserts

   5' iRFP arm
   eGFP with I-Sce I TS
   +3 mCherry
   3' iRFP arm
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   embedded I-Sce I TS from 163-185bp, truncated 25 …

   Promoter

   SFFV

   Available since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a_PTK2b

   Plasmid

   #224237

   Purpose

   Expression of human PTK2b Kinase in E. coli

   Depositor

   Andrew Mesecar

   Insert

   ptk2b (PTK2B Human)
   

   Use

   Tags

   6xHis and 6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-4T1-USP11

   Plasmid

   #78849

   Purpose

   To purify GST-tagged recombinant USP11 protein

   Depositor

   Jaewhan Song

   Article

   Lee et al Cell Death Differ. 2015 Sep;22(9):1463-7

   Insert

   USP11 (USP11 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

10. p11d-tRPA-32Asp8

    Plasmid

    #102617

    Purpose

    Expression of human RPA with aspartic acid substituted phosphorylation domain

    Depositor

    Marc Wold

    Article

    Binz et al J Biol Chem. 2003 Sep 12;278(37):35584-9

    Inserts

    RPA1 (RPA1 Human)
    RPA3 (RPA3 )
    RPA2 (RPA2 )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    with aspartic acid substituted phosphorylation do…

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. RhoA

    Plasmid

    #55699

    Purpose

    expression of mouse RhoA

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    RhoA (Rhoa Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-5X1 Ash2L full

    Plasmid

    #15549

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    Ash2L (ASH2L Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pCAT205

    Plasmid

    #134882

    Purpose

    Bacterial expression

    Depositor

    Thomas Howard

    Article

    Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. MP2

    Plasmid

    #69628

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E. coli PBAD

    Available since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. EPHA7 (2REI)

    Plasmid

    #25148

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    epha7.590.899 (EPHA7 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

16. pHH0103_PCMT1_PCMT

    Plasmid

    #109932

    Purpose

    Protein expression and purification of PCMT1_PCMT

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    PCMT1_PCMT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET15b-Casp8 delta DED

    Plasmid

    #11827

    Depositor

    Guy Salvesen

    Article

    Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

    Insert

    Caspase 8 minus Death Effecter Domain (CASP8 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Minus Death Defecter Domain (aa 1-216)

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA.3.1.ADRB2-NP

    Plasmid

    #134376

    Purpose

    Nanoluc complementation assay. Expression of adrenoceptor beta 2 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of signal sequence and Flag epitope at N terminus of ADRB2.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    ADRB2-NP (ADRB2 Human)
    

    Use

    Tags

    Flag, natural peptide of nanoluciferase, and sign…

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    Aug. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. ibpAB-GFP

    Plasmid

    #109390

    Purpose

    GFP cassette under the control of the ibpAB sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pibpAB-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-RT007-GCN4scFV-VB

    Plasmid

    #163387

    Purpose

    2nd generation lentiviral vector to express anti-GCN4 module in the G-baToN system

    Depositor

    Monte Winslow

    Article

    Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

    Insert

    antiGCN4scFV-VEEGFRtm-BFP (GCN4scFV-VB)
    

    Use

    Lentiviral

    Tags

    BFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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