31,800 results
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Plasmid#124366PurposeGfpmut2 constitutively expressed using the Ppen promoter for expression in B. subtilisDepositorAvailable SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only
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pBbB2a-GFP
Plasmid#35343DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialPromoterTetAvailable SinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
Spacer Switch Plasmid
Plasmid#215346PurposeFacilitates the change in spacer sequence to Neisseria gonorrhoeae encoding the Nla CRISPRi system.DepositorInsertOpaD spacer; Chloramphenicol Resistance Cassette
UseCRISPRExpressionBacterialAvailable SinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF1-Pls4-sfGFP
Plasmid#205751PurposeExpresses sfGFP under IPTG inducible promoter Pls4 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. High strength.DepositorTypeEmpty backboneUseShuttle vector gram+ gram-ExpressionBacterialPromoterPls4 (Phyperspank mutant, IPTG inducible)Available SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKEK3230
Plasmid#234102PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertFuGFP
ExpressionBacterialPromoterpJ23100-BsaIAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBad33_IS605_reRNA
Plasmid#205321PurposeBacterial expression of IS605 TnpB and its reRNADepositorInsertIS605 TnpB
ExpressionBacterialAvailable SinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pSEVA228-pro4IUPi
Plasmid#122018PurposeExpresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.DepositorInsertsExpressionBacterialMutationCodon optimized for E. coliPromoterpro4Available SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
Drp1-EGFP-StrepII
Plasmid#174429PurposeExpresses human Drp1 Isoform 3 with EGFP at C-terminus in bacteriaDepositorInsertDrp1
TagsEGFP-StrepIIExpressionBacterialMutationNonePromoterT7Available SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTXB3-Flag PLK1 T210D
Plasmid#68272Purposebacterial expression of Plk1 T210D mutantDepositorInsertPLK1 (PLK1 Human)
TagsFlag and Mxe intein/chitin binding domainExpressionBacterialMutationT210DPromoterT7Available SinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
EGFP-p300 (D1399Y)
Plasmid#191763PurposeExpression of EGFP fused to catalytically dead core p300 histone acetyltransferase (D1399Y)DepositorInsertFRB-EGFP-p300 core (D1399Y) (EP300 Human)
TagsEGFP (N-terminal of p300 core (D1399Y)) and FRB (…ExpressionBacterial and MammalianMutationcatalytic D1399Y mutation in p300PromoterEF1alphaAvailable SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mVenus ME
Plasmid#103989PurposePlasmid encoding mVenus MEDepositorInsertmVenus ME
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F46L, F64L, …PromoterproCAvailable SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-Zn2
Plasmid#174805PurposeBacterial expression of isolated PARP1 Zn2 domainDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-Zn3
Plasmid#174806PurposeBacterial expression of isolated PARP1 Zn3 domainDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJCSUP35
Plasmid#1088DepositorAvailable SinceMay 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET24b-SUMO-BG2.4
Plasmid#199438Purposeexpresses BAmGreen2.4 in bacterial cells with a SUMO tagDepositorInsertBAMGreen2.4
Tagshis tag and sumoExpressionBacterialPromoterT7Available SinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMega-MaFRSA
Plasmid#200226PurposeThe plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..DepositorInsertsPyrrolysyl-tRNA synthetase
pyrrolysyl-tRNA
TagsnoneExpressionBacterialMutationmutated Asparagine 166 to Alanine and Valine 168 …PromoterproK-lacO and tacIAvailable SinceJune 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEB1-moxGFP
Plasmid#103984PurposePlasmid encoding moxGFPDepositorInsertmoxGFP
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (S30R, Y39N, …PromoterproCAvailable SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEB1-moxCerulean3
Plasmid#103975PurposePlasmid encoding moxCerulean3DepositorInsertmoxCerulean3
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F64L, Y66W, …PromoterproCAvailable SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp15
Plasmid#156481PurposeBacterial expression of Sars-CoV2 Nsp15 protein with His-tag and GST-tagDepositorInsertNsp15 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only