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pTn7-SCOUT12.G

Plasmid

#201000

Purpose

pLv1-pUC18R6KT-miniTn7-Km-sfGFP, KmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Insert

superfolder GFP

Expression

Bacterial

Promoter

J23104

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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AviTag-SpyTag003-MBP

Plasmid

#184227

Purpose

Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.

Depositor

Mark Howarth

Article

Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

Insert

AviTag-SpyTag003-MBP

Tags

AviTag, His6, SpyTag003, and Thrombin cleavage si…

Expression

Bacterial

Promoter

T7

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCAD39_mIRG1_4-462_pvp008

Plasmid

#124873

Purpose

Expresses mouse CAD (Irg1) in E.coli

Depositor

Konrad Buessow

Article

Chen et al Proc Natl Acad Sci U S A. 2019 Sep 23. p

Insert

cis-aconitate decarboxylase (Acod1 Mouse)

Tags

StrepTag and TEV site

Expression

Bacterial

Mutation

deleted amino acids 1-3 and 463-end.

Promoter

T7

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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bdSUMO-ZF5.3-GFP-HiBit

Plasmid

#170992

Purpose

For bacterial expression of bdSUMO tagged muGFP with ZF5.3 peptide and NanoLuc complementation peptide variant #86

Depositor

Angus Johnston

Article

Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

Insert

14x-His-bdSUMO-muGFP-HiBiT

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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p3993 pET ubcH7

Plasmid

#8654

Depositor

Peter Howley

Article

Scheffner et al Proc Natl Acad Sci U S A 1994 Sep 13;91(

Insert

UbcH7 (UBE2L3 Human)

Expression

Bacterial

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pGT-Ah7

Plasmid

#122574

Purpose

MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

catP/sfGFP

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pQTEV-HSPB8

Plasmid

#34710

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

heat shock 22 kDa protein 8 (HSPB8 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pDEST14-ARL3

Plasmid

#67376

Purpose

Expression of native hArl3 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL3 (ARL3 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pRFP

Plasmid

#26924

Depositor

Ron Weiss

Article

Basu et al Nature. 2005 Apr 28. 434(7037):1130-4.

Insert

LacI, HcRed

Expression

Bacterial

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pCURE

Plasmid

#23013

Depositor

James Bowie

Article

Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

Insert

I-CreI homing endonuclease

Expression

Bacterial

Mutation

I-CreI expression is controlled by the arabinose …

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pBbdCas9S

Plasmid

#149638

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, empty

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pET28_ancCas9d_Plasmid

Plasmid

#239444

Purpose

Expression plasmid codon-optimized for E. coli

Depositor

David Taylor

Article

Ocampo et al Nat Commun. 2025 Jan 7;16(1):457. doi: 1

Insert

ancCas9d

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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TurboRFP-pBAD

Plasmid

#54858

Purpose

Localization: Protein Expression Vector , Excitation: 553, Emission: 574

Depositor

Dmitriy Chudakov, Michael Davidson, Dorus Gadella

Article

Merzlyak et al Nat Methods. 2007 Jul;4(7):555-7. Epub 2

Type

Empty backbone

Tags

6xHis and TurboRFP

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pSAP

Plasmid

#206429

Purpose

A vector backbone domesticated for SapI (pCC1BAC-based), contains elements for selection, maintenance, and propagation in S. cerevisiae (TRP1) and E. coli (cat).

Depositor

Bogumil Karas

Article

Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pTW335l

Plasmid

#246590

Purpose

G. hirsutum Rubisco gene

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

G. hirsutum RbcL, RbcS 6xhis (rbcL G. hirsutum RbcL)

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF_AaRbcLS

Plasmid

#229505

Purpose

Expresses Anthoceros agrestis Rubisco large and small subunit (C terminus hexahistidine)

Depositor

Laura Gunn

Article

Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

Inserts

Rubisco large subunit
Rubisco small subunit

Tags

hexahistidine

Expression

Bacterial

Mutation

N terminus chloroplast transit peptide truncation

Promoter

T7

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCDF_AaRaf1/RbcX2/BSD2/RbcX1

Plasmid

#229513

Purpose

Expresses Anthoceros agrestis chaperones: Raf1,RbcX2,BSD2,RbcX1

Depositor

Laura Gunn

Article

Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

Inserts

Rubisco accumulation factor 1
RbcX2
BSD2
RbcX1

Expression

Bacterial

Mutation

N terminus chloroplast transit peptide truncation

Promoter

T7

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTW335m

Plasmid

#237328

Purpose

expresses M. truncatula RbcL RbcS-6xHis

Depositor

Tina Wang

Article

Archer et al J Exp Bot. 2025 Mar 31:eraf140. doi: 10.

Inserts

M. truncatula RbcL
M. truncatula RbcS-6xHis

Tags

6xHis and None

Expression

Bacterial

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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M-NylC_pET28a

Plasmid

#215409

Purpose

Expression construct for M-NylC gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

M-NylC

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCC1-4k-BBclone2

Plasmid

#137070

Purpose

E. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Inserts

BB0323
P13
DipA
Lmp1

Expression

Bacterial

Mutation

wild-type, full-length (signal-peptide-containing…

Available Since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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