33,449 results
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Plasmid#138180PurposeExpresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only
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PJQ47
Plasmid#134646PurposeContains Promoter 1 with bsa1 cut sites for inserting sgRNADepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterAnderson BBa_J23117Available sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNCST-mAvicFP1
Plasmid#129509PurposeExpresses mAvicFP1 constitutively in E. coli (most strains)DepositorInsertmAvicFP1
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promote…Available sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-Munc18-1wt
Plasmid#135550PurposeExpress rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertMunc18-1wt
Tags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJH181.3
Plasmid#135076PurposeCoryne. glutamicum HA production plasmidDepositorInserthasA-hasC
ExpressionBacterialAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOD3-proD/Lux-nat
Plasmid#120324PurposeBacterial luminescenceDepositorInsertLux operon
ExpressionBacterialPromoterproDAvailable sinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHIS-GB1-BP1
Plasmid#133427PurposeHuman BP1 fragment of SREBF1 with GB1 solubility tag in vector for in vitro expression, with T7 promoter.DepositorInsertSREBF1 (SREBF1 Human)
ExpressionBacterialMutationSREBP1a amino acids 17-42 fused to GB1 solubilit…Available sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30-GBFusion1
Plasmid#133438PurposeStreptococcus GB1 domain in vector for bacterial expression.DepositorPublicationInsertGB1 domain
ExpressionBacterialMutationThis fragment encodes only aa 1 to 56.Available sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rha
Plasmid#129390PurposeDerived from mini-CTX1. An integrase vector that can integrate the rhamnose-inducible promoter system into the chromosome of a host bacterium.DepositorInsertRhamnose-inducible promoter
ExpressionBacterialMutationN/APromoterPrhaBADAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJBL7025
Plasmid#128810PurposeFluoride riboswitch-regulated catechol 2,3-dioxygenaseDepositorInsertFluoride riboswitch-regulated catechol 2,3-dioxygenase
UseSynthetic BiologyExpressionBacterialAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
K560_AviC_coBirA
Plasmid#129765PurposeExpresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assaysDepositorInsertKHC (Khc Fly)
TagsAvi-tag GLNDIFEAQKIEWH and His6ExpressionBacterialMutationTruncation at 559Available sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
(SUMO)5
Plasmid#126949PurposeBacterial expression plasmid for (SUMO)5 client to assess SUMO-SIM scaffolds.DepositorInsert5 repeats of human SUMO3 separated by (GGS)4 (SUMO3 Human)
ExpressionBacterialPromoterunknownAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAD-SPS6-Pman
Plasmid#133906PurposeFluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.DepositorInsertsfGFP(SPS6)
UseE.coli/bacillus spp. shuttle vectorExpressionBacterialPromoterPmanAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB1-eGFP-6xHis
Plasmid#130981PurposeBacterial expression of mouse EB1-GFP-6xHis for protein purification.DepositorAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBXNH3CA_MBP
Plasmid#132700Purposeexpresses Avi-tagged MBP in E.coli, to use as positive control in ELISADepositorInsertMaltose binding Protein
TagsAvi and HisExpressionBacterialPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-Sth1Cas9
Plasmid#129552PurposeContains codon-optimized Sth1Cas9 and gRNA for streptomycesDepositorInsertSth1Cas9
ExpressionBacterialPromoterrpsL(XC)-BbsIAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ERG_pet28a
Plasmid#131642PurposeBacterial expression of ETS gene ERGDepositorAvailable sinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
Sender plasmid
Plasmid#127677PurposeEncodes the synthase LuxI for the quorum-sensing molecule C6-HSL.DepositorInsertLuxI
UseSynthetic BiologyTagsLVAExpressionBacterialPromoterpT7Available sinceSept. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBBR5pemIKpKan
Plasmid#129271PurposeBroad host-range plasmid with toxin-antitoxin system for maintenance in the absence of antibiotic selection, and constitutive promoterDepositorTypeEmpty backboneExpressionBacterialAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_LAMA-G97 (for GFP)
Plasmid#130715PurposePlasmid encoding the bacterial expression of LAMA-G97 for GFPDepositorInsertLAMA-G97
TagsHisx10ExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by