34,232 results

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pHH0103_RAD51_Rad51

Plasmid

#110031

Purpose

Protein expression and purification of RAD51_Rad51

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RAD51_Rad51 (RAD51 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_DYNLL1_Dynein-light

Plasmid

#110006

Purpose

Protein expression and purification of DYNLL1_Dynein-light

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DYNLL1_Dynein-light (DYNLL1 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHH0103_PABPC1_PABP

Plasmid

#109931

Purpose

Protein expression and purification of PABPC1_PABP

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PABPC1_PABP

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_YWHAZ_14-3-3

Plasmid

#109808

Purpose

Protein expression and purification of YWHAZ_14-3-3

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

YWHAZ_14-3-3 (YWHAZ Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNAQ_G-alpha

Plasmid

#109809

Purpose

Protein expression and purification of GNAQ_G-alpha

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNAQ_G-alpha (GNAQ Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNAI2_G-alpha

Plasmid

#109791

Purpose

Protein expression and purification of GNAI2_G-alpha

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNAI2_G-alpha (GNAI2 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNA15_G-alpha

Plasmid

#109782

Purpose

Protein expression and purification of GNA15_G-alpha

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNA15_G-alpha (GNA15 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNA500

Plasmid

#149640

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pUC18/promSau/Lysostaphin

Plasmid

#125766

Purpose

Production of staphylolytic protein lysostaphin from a constitutive promoter

Depositor

Benedykt Wladyka

Publication

Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

Insert

lysostaphin

Expression

Bacterial

Mutation

sequence corresponding to the mature form protein

Promoter

pemIK constitutive promoter

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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T7 promoter + filler DNA reporter plasmid

Plasmid

#156454

Purpose

A mutational reporter plasmid that can gain Kanamycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

Depositor

Matthew D. Shoulders

Publication

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Inserts

NeoR/KanR
TcR

Expression

Bacterial

Mutation

ATG start codon mutated to ACG

Promoter

Em7 and tet

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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R726-M01-892: His6-Halotag-tev-Hs.RAF1(52-131)

Plasmid

#159696

Purpose

E. coli protein expression of His6-Halotag-tev-Hs.RAF1(52-131)

Depositor

Dominic Esposito

Publication

Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

Insert

His6-Halotag-tev-Hs.RAF1(52-131) (RAF1 Human)

Tags

His6-Halotag-tev

Expression

Bacterial

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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R989-X02-566: His6-MBP-tev-G-Hs.KRAS4b(1-169)

Plasmid

#159539

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169)

Depositor

Dominic Esposito

Publication

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) (KRAS Human)

Tags

His6-MBP-tev

Expression

Bacterial

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pACDura3

Plasmid

#138711

Purpose

Expresses group II intron Ll.LtrB and IEP (LtrA) from Lactoccus lactis under control of T7 promoter. Ll.LtrB is retargeted to insert into 635s locus of LacZ gene and ura3 gene is cloned inside.

Depositor

Víctor de Lorenzo

Publication

Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

Insert

ura3 gene (URA3 Budding Yeast)

Expression

Bacterial

Mutation

optimized ura3 gene for expression in Escherichia…

Promoter

pEM7 promoter

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSEVA421-Cas9tr

Plasmid

#138709

Purpose

Plasmid constitutively expressing the Cas9 nuclease and the non-coding tracrRNA from S. pyogenes

Depositor

Víctor de Lorenzo

Publication

Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

Insert

tracrRNA-Cas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSB3C5-phzSM

Plasmid

#141105

Purpose

Contains a phzS-phzM operon BioBrick

Depositor

Martin Buck

Publication

Clifford et al Chem Sci. 2021 Jan 15;12(9):3328-3338. d

Insert

phzSM

Expression

Bacterial

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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TwinStrep-SUMO-NLS-BhCas12b_v4

Plasmid

#158615

Purpose

For protein expression of BhCas12b_v4

Depositor

Feng Zhang

Publication

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Insert

NLS-BhCas12b_v4

Tags

6xHIS-TwinStrep-SUMO

Expression

Bacterial

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-6His-SUMO-Nsp14

Plasmid

#154956

Purpose

Expresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

Depositor

Justin Kollman

Insert

6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)

Tags

6His-Thrombin-SUMO

Expression

Bacterial

Promoter

T7 Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pETM33_Nsp10

Plasmid

#156479

Purpose

Bacterial expression of Sars-CoV2 Nsp10 protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Publication

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp10 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL725

Plasmid

#140395

Purpose

Expresses SmtB protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Publication

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-SmtB-T

Tags

TEV-6XHis

Expression

Bacterial

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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WARS_pNIC-CTB10H

Plasmid

#153058

Purpose

Expression construct for producing Avi tagged Tryptophanyl-tRNA synthetase

Depositor

Susanne Gräslund

Insert

WARS (WARS1 Human)

Tags

TEV-Avi-His10

Expression

Bacterial

Promoter

T7

Available since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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