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Showing: 5741 - 5760 of 29457 results

  8. pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer

    Plasmid
    #172501
    Purpose
    Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers
    Depositor
    Jonathan Gootenberg, Omar Abudayyeh
    Article
    Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-
    Insert
    DisCas7-11 full locus with Golden gate acceptor site for spacer
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    172501-plasmid-map-sequence-id-344918

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  11. pET28a-His-MinD_MinE

    Plasmid
    #133621
    Purpose
    Expression vector for the Escherichia coli MinD with an N-terminal His-tag for purification. MinE is encoded in the ORF to avoid toxicity when overexpressing MinD, but MinE is not tagged.
    Depositor
    Petra Schwille
    Article
    Loose et al Science. 2008 May 9;320(5877):789-92. do
    Insert
    His-MinD_MinE (minD, minE E. coli)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    133621-plasmid-map-sequence-id-271463

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  12. pKL004

    Plasmid
    #98920
    Purpose
    Constitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli.
    Depositor
    Joel Kralj
    Article
    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11
    Insert
    GCaMP6f tethered to mRuby
    Use
    Tags
    His and mRuby3
    Expression
    Bacterial
    Mutation
    Promoter
    BBa_J23118 - Strong Constitutive
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    98920-plasmid-map-sequence-id-189873

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  19. pKB222

    Plasmid
    #170012
    Purpose
    Encodes LacI-controlled expression of surface-displayed LL-37 and PvirK-mNeonGreen reporter
    Depositor
    Jeffrey Tabor
    Article
    Brink et al bioRxiv
    Inserts
    mNeonGreen
    LL-37
    Use
    Synthetic Biology
    Tags
    Lpp, OmpA, and Tether
    Expression
    Bacterial
    Mutation
    Promoter
    Ptac and PvirK
    Available since
    March 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    170012-plasmid-map-sequence-id-335945

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  20. pAC-crRNA-Cm

    Plasmid
    #158711
    Purpose
    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    eforCP (for crRNA cloning)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    TetO
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    158711-plasmid-map-sequence-id-325466

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Showing: 5741 - 5760 of 29457 results