30,807 results

1. pHCKan-yibDp-ald*-alaE

   Plasmid

   #134939

   Purpose

   Low phosphate inducible alanine dehydrogenase and alanine exporter

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   alanine dehydrogenase and L-alanine exporter
   

   Expression

   Bacterial

   Promoter

   yibD

   Available Since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST17-UbE2F

   Plasmid

   #15800

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2F (UBE2F Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pET28a-His6-ODC-Ctag

   Plasmid

   #163614

   Purpose

   Expresses truncated human Ornithine Decarboxylase in bacterial cells for binding to Ornithine Decarboxylase Antizyme.

   Depositor

   Mark Howarth

   Article

   Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

   Insert

   ODC (ODC1 Human)
   

   Tags

   C-tag and His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. p4305

   Plasmid

   #198150

   Purpose

   bacterial expression of sifA::HaloTag fusion

   Depositor

   Michael Hensel

   Insert

   PsifA sifA::L16::HaloTag::HA (sifA Salmonella enterica serovar Typhimurium)
   

   Tags

   HaloTag-HA

   Expression

   Bacterial

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOmpF

   Plasmid

   #42606

   Depositor

   Bryan Berger

   Article

   Su et al Protein Sci. 2013 Jan 24. doi: 10.1002/p

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pFastBac_human-IL-18

   Plasmid

   #214338

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18 (IL18 Human)
   

   Tags

   His-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. MT1E

   Plasmid

   #105702

   Purpose

   Bacterial expression plasmids for production of recombinant metallothionein 1e

   Depositor

   Artur Krężel

   Article

   Peris-Diaz et al J Proteome Res. 2021 Jan 1;20(1):776-785

   Insert

   Metallothionein 1e (MT1E Human)
   

   Tags

   Chiting Binding Domanin-Intein

   Expression

   Bacterial

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS PKC theta

   Plasmid

   #8426

   Depositor

   Frederic Mushinski

   Article

   Mischak et al FEBS Lett 1993 Jul 12;326(1-3):51-5.

   Insert

   Mouse Protein Kinase C theta (Prkcq Mouse)
   

   Expression

   Bacterial

   Available Since

   Nov. 22, 2005

   Availability

   Academic Institutions and Nonprofits only

9. pIOM15

   Plasmid

   #20869

   Depositor

   Carmen Beuzón

   Article

   Ortiz-Martin et al J Microbiol Methods. 2006 Dec . 67(3):39

   Insert

   aphA::aadA
   

   Expression

   Bacterial

   Available Since

   July 15, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pTH1-SaSrtA

    Plasmid

    #64973

    Purpose

    Expresses the wild type of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59

    Promoter

    T7 promoter

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. KLHL40 (KBTBD5A-c006)

    Plasmid

    #98233

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KBTBD5A (KLHL40 Human)
    

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGST2-GST-TEV-NDP52

    Plasmid

    #187828

    Purpose

    Plasmid for the expression and purification of GST-TEV-NDP52 in E. coli Rosetta pLysS. Internal reference: SMC1586

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Tags

    GST-TEV

    Expression

    Bacterial

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. ykgMO-IGS-LuxCDABE

    Plasmid

    #207232

    Purpose

    Calprotectin sensing promoter with luciferase reporter

    Depositor

    Arthur Prindle

    Article

    Xia et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    ykgMO-IGS
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEaaK-NanR-J23113-pNanA-CadC-pCadBA-GFP

    Plasmid

    #200277

    Purpose

    Characterisation of the sialic acid-responsive circuit with fluorescent reporter

    Depositor

    Matthew Chang

    Article

    Koh et al Nat Commun. 2022 Jul 4;13(1):3834. doi:

    Inserts

    CadC
    NanR
    

    Expression

    Bacterial

    Available Since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. ACACA

    Plasmid

    #39083

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ACACA (ACACA Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pBTR(hCc)

    Plasmid

    #61026

    Purpose

    Expresses horse holocytochrome c in E. coli

    Depositor

    Gary Pielak

    Article

    Patel et al Protein Expr Purif. 2001 Jul;22(2):220-4

    Insert

    horse cytochrome c (CYCS Equus*)
    

    Tags

    None

    Expression

    Bacterial

    Mutation

    *see comments

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pMRE-Tn7-140

    Plasmid

    #118556

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    TagBFP2
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJH643

    Plasmid

    #172168

    Purpose

    Reporter plasmid for pylBCD variant in pBAD backbone

    Depositor

    Matthew Bennett

    Article

    Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

    Insert

    Para sfGFP.3TAG; Plpp pylS; PproK pylT; AraC; Cam
    

    Expression

    Bacterial

    Mutation

    sfGFP(N39*, N135*, Y151*)

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJUMP27ts-1A(sfGFP)

    Plasmid

    #126975

    Purpose

    Level 1 vector. sfGFP reporter. Thermosensitive origin pSC101 (low copy number)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    RepA A56V thermosensitive mutation

    Available Since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTXB1-EXO1b

    Plasmid

    #68267

    Purpose

    Bacterial expression of human EXO1

    Depositor

    Stefano Ferrari

    Article

    El-Shemerly et al Cancer Res. 2005 May 1;65(9):3604-9.

    Insert

    EXO1 (EXO1 Human)
    

    Tags

    Mxe intein/chitin binding domain

    Expression

    Bacterial

    Mutation

    b form, codons 1-846. Please see depositor commen…

    Promoter

    T7

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only