31,059 results
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Plasmid#86834Purposebacterial expression of anti-CRISPR protein AcrIIA1DepositorInsertacrIIA1
ExpressionBacterialPromoteraraBADAvailable SinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbA8a-RFP
Plasmid#35271DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterBADAvailable SinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pC0060 AspCas13 (B04) His6-TwinStrep-SUMO-BsaI
Plasmid#115210PurposeExpresses AspCas13b from a SUMO-tagged bacterial expression constructDepositorInsertAspCas13
TagsSUMOExpressionBacterialAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJK635
Plasmid#72473PurposepET28a modified to contain TEV protease fusion tag instead of T7 tagDepositorTypeEmpty backboneTagsHis6 and TEV protease cleavage siteExpressionBacterialPromoterT7Available SinceJan. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-CLIP-tag_fast
Plasmid#167275PurposeOverexpression of the fast variant (E30R) of CLIP-tag in E. coliDepositorInsertCLIPf-tag
TagsHis x10ExpressionBacterialMutationE30RPromoterT7Available SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-PSmOrange2
Plasmid#34957DepositorInsertPSmOrange2
ExpressionBacterialMutationR17H, R36H, F64I, Q194L, A224S, G226A (mutations …Available SinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
R726-M01-892: His6-Halotag-tev-Hs.RAF1(52-131)
Plasmid#159696PurposeE. coli protein expression of His6-Halotag-tev-Hs.RAF1(52-131)DepositorAvailable SinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
SiriusGFP
Plasmid#123201PurposeWe have developed a variant of eGFP, SiriusGFP, that shows over a two fold increase in photostability with utility in methods requiring sustained or high intensity excitation as in 4D confocal or SIM.DepositorInsertSiriusGFP
UseMouse TargetingTagsN/AExpressionBacterial and MammalianMutationeGFP-S30R/Y39N/F99S/N105T/S147R/M153T/V163A/S205V…PromoterCMVAvailable SinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPGC-K
Plasmid#174581PurposepOPIN-GG expression vector compatible with C-terminal tagging, Kanamycin resistantDepositorTypeEmpty backboneExpressionBacterialAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
GST-FOXO3a WT
Plasmid#1790DepositorAvailable SinceDec. 8, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAM5087
Plasmid#85123PurposePtrc-YFP subcloned from pAM5080 into NSII-Km vector.DepositorInsertYFP
ExpressionBacterialAvailable SinceJune 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
UHRF1
Plasmid#28163PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertUHRF1 (UHRF1 Human)
TagsHis tag with TEV cleavageExpressionBacterialMutationtandem tudor domainAvailable SinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET51b(+)_H-Luc
Plasmid#113924PurposePlasmid encoding the bacterial expression of the chimeric HaloTag7-NanoLucCP fusion dubbed H-Luc, for BRETDepositorInsertH-Luc
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 vatD
Plasmid#112885PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertvatD
ExpressionBacterialPromoterPblaAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP1 vph
Plasmid#112893PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertvph
Tags6xHisExpressionBacterialPromoterPblaAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc3-HISNDHFR
Plasmid#63976PurposePosition 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneTags6xHis; TEV cleavableExpressionBacterialPromoterT7Available SinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFS_0235-pET30-Fusicatenibacter_saccharivorans_ARRAY2_FaqI
Plasmid#116954PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
ExpressionBacterialPromoterT7Available SinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-hOPTN
Plasmid#23053DepositorAvailable SinceMay 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
human p53-(311-393)
Plasmid#24869DepositorAvailable SinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET28a-PKM2-R399E
Plasmid#122691PurposeExpress His-tagged PKM2 (R399E) protein in bacteria.DepositorAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only