30,808 results

1. pET15b-Casp8 delta DED

   Plasmid

   #11827

   Depositor

   Guy Salvesen

   Article

   Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

   Insert

   Caspase 8 minus Death Effecter Domain (CASP8 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Minus Death Defecter Domain (aa 1-216)

   Available Since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pDEST14-Avitag-SpyCatcher002

   Plasmid

   #102828

   Purpose

   Bacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylation

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   Avitag-SpyCatcher002
   

   Tags

   Avitag, GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. DHX9A

   Plasmid

   #42456

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   DHX9A (DHX9 Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   See Comments

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pAWPSG-Ptet-nCas9-BE

   Plasmid

   #195739

   Purpose

   Express APOVEC1-nCas9(D10A)-UGI using aTC inducible system in Methanotroph or E. coli

   Depositor

   Seung-Goo Lee

   Article

   Jeong et al Chem Eng J, 2023

   Insert

   Tet repressor protein / APOBEC1-nCas9(D10A)-UGI
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   Ptet(Tetracycline inducible protein)

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Human USP30 (64-502, construct 8, MG-26-21)

   Plasmid

   #110745

   Purpose

   Bacterial expression of human USP30 (construct 8)

   Depositor

   David Komander

   Article

   Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

   Insert

   USP30 (USP30 Human)
   

   Tags

   N-His6-3C-GST-3C

   Expression

   Bacterial

   Mutation

   F348D, M350D, I353E + insertion deletion: 64-357;…

   Promoter

   T7

   Available Since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. mT-Sapphire-pBAD

   Plasmid

   #54571

   Purpose

   Localization: Protein Expression Vector , Excitation: 399, Emission: 511

   Depositor

   Robert Campbell, Michael Davidson

   Article

   Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1

   Type

   Empty backbone

   Tags

   6xHis and mT-Sapphire

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pEXP5-NT/pT7 LuxI

   Plasmid

   #193626

   Purpose

   Constitutive T7 RNAP-mediated expression of LuxI 3OC6-HSL synthase for Lux quorum sensing system. LuxI sequence is codon optimized for E. coli.

   Depositor

   Dora Tang

   Article

   Gonzales et al ChemSystemsChem 2023

   Insert

   LuxI
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7 RNAP promoter

   Available Since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pet42c(+)-GST-USP13

   Plasmid

   #61749

   Purpose

   express N-terminally GST tagged human USP13

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   USP13 (USP13 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

9. WGA-D

   Plasmid

   #17989

   Depositor

   Natasha Raikhel

   Article

   Smith et al Plant Mol Biol. 1989 Nov . 13(5):601-3.

   Insert

   wheat germ agglutinin isolectin D
   

   Expression

   Bacterial

   Mutation

   N/A

   Available Since

   July 10, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pBAD/HisB (TIREVOL)-sfGFP

    Plasmid

    #189675

    Purpose

    titratable expression of sfGFP in bacteria

    Depositor

    Daniel Daley

    Article

    Shilling et al Synth Biol (Oxf). 2022 Jul 5;7(1):ysac00

    Insert

    sfGFP
    

    Tags

    His-T7-EK

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGWB508-cTP-sfGFP

    Plasmid

    #213863

    Purpose

    Binary vector for the expression of sfGFP flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

    Insert

    Plastid transit peptide fused sfGFP
    

    Expression

    Bacterial and Plant

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28b-pchG WT

    Plasmid

    #87107

    Purpose

    expresses PchG WT, the thiazolinyl imine reductase from Pseudomonas aeruginosa

    Depositor

    Audrey Lamb

    Article

    Meneely et al Biochemistry. 2016 Sep 27;55(38):5423-33

    Insert

    pchG WT
    

    Tags

    his-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. ETV4_pet28a

    Plasmid

    #131645

    Purpose

    Bacterial expression of ETS gene ETV4

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    ETV4 (ETV4 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJOE6261.2

    Plasmid

    #135079

    Purpose

    P. putida KT2440 rescue construct

    Depositor

    Josef Altenbuchner

    Article

    Graf et al Appl Environ Microbiol. 2011 Aug;77(15):

    Insert

    upp (upp Pseudomonas putida)
    

    Expression

    Bacterial

    Available Since

    Feb. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS-HxB.S

    Plasmid

    #65415

    Purpose

    contains complete cDNA for TNC small splice variant flanked by NotI-KpnI

    Depositor

    Harold Erickson

    Article

    Aukhil et al J Biol Chem. 1993 Feb 5;268(4):2542-53.

    Insert

    tenascin C small splice variant (TNC Human)
    

    Expression

    Bacterial

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSFDuet-1_mNeongreen-Etanercept

    Plasmid

    #182420

    Purpose

    Bacterial expression of mNeongreen-Etanercept fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    mNeongreen-Etanercept (TNFRSF1B Human)
    

    Tags

    mNeongreen

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHK316

    Plasmid

    #235474

    Purpose

    Inducible gene VIII by Ptet promoter

    Depositor

    Yolanda Schaerli

    Article

    et al Nat Commun 16, 3569 (2025)

    Insert

    phage M13 geneVIII
    

    Expression

    Bacterial

    Available Since

    May 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAROTENE5X

    Plasmid

    #208816

    Purpose

    Encodes bacterial crtEBIY operon under the control of synthetic promoter responsive to JUB1-derived artificial transcription factor in bacterial cell

    Depositor

    Marc Erhardt

    Article

    Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:

    Insert

    Five copies of JUB1 binding site within the synthetic promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    JUB1 TF-responsive promoter

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETM6-T7-VHH72

    Plasmid

    #202487

    Purpose

    Expresses the nanobody VHH72 with T7 induction

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    nanobody VHH72
    

    Tags

    6XHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDB179-LCC

    Plasmid

    #218686

    Purpose

    Encodes codon optimized LCC enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    LCC
    

    Tags

    10xHis

    Expression

    Bacterial

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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