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pTWIN1-His6-Ssp-Htt2-90-43Q-P90C

Plasmid

#114626

Purpose

Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 43 polyQ repeat

Depositor

Hilal Lashuel

Article

Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

Insert

Huntingtin Exon1 fragment (HTT Human)

Tags

His6-Ssp DnaB intein

Expression

Bacterial

Mutation

P90C; 43 polyQ repeat

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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human TF cDNA

Plasmid

#15455

Depositor

Nigel Mackman

Article

Mackman et al FASEB J. 1995 Jul . 9(10):883-9.

Insert

Tissue factor (F3 Human)

Use

Luciferase

Expression

Bacterial

Available Since

July 27, 2007

Availability

Academic Institutions and Nonprofits only
pJ2018

Plasmid

#140665

Purpose

dCas9, LuxR & Csy4

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

dCas9, LuxR & Csy4

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pJOE6227.1

Plasmid

#135078

Purpose

An integration vector for the chromosomal deletion of the upp gene in P. putida KT2440; carries the upp deletion cassette.

Depositor

Josef Altenbuchner

Article

Graf et al Appl Environ Microbiol. 2011 Aug;77(15):

Insert

up- and downstream regions of upp (upp Pseudomonas putida)

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pMal-E6LLO

Plasmid

#51281

Purpose

Expresses a fibronectin fusion with listeriolysin targeting EGFR in bacteria

Depositor

Dane Wittrup

Article

Pirie et al Mol Cancer Ther. 2013 Sep;12(9):1774-82.

Insert

E6LLO

Expression

Bacterial

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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H11-CAG-loxP-FNF-bpA-3pA-loxP-td-sfGFP

Plasmid

#180155

Purpose

td-sfGFP cre reporter with Hipp11 recombineering homology arms

Depositor

Mario Capecchi

Insert

td-sfGFP

Use

Cre/Lox and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pHCL147

Plasmid

#134454

Purpose

TetR, lacI-Plac-ssdsbA-mscarC, Integrative plasmid for the expression of fully-secreted prey. C-terminal mScarlet-I fusion.

Depositor

Thomas Bernhardt

Article

Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111

Insert

linker-mScarlet-I for C-terminal fusion

Expression

Bacterial

Promoter

plac

Available Since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pBBR1-kan-Ptet-RecETh1h2h3h4_agrob6

Plasmid

#180428

Purpose

Genome engineering of Agrobacterium

Depositor

Jun Fu

Article

Bian et al Appl Environ Microbiol. 2022 Mar 8;88(5)

Insert

RecETh1h2h3h4_agrob6

Expression

Bacterial

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMEXC3H

Plasmid

#110080

Purpose

Analogous to pMINTC3H, but containing oriM for episomal replication in mycobacteria.

Depositor

Markus Seeger

Article

Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

Type

Empty backbone

Tags

3C cleavage site - His10 Tag

Expression

Bacterial

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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Dlx2

Plasmid

#15537

Depositor

John Rubenstein

Article

Porteus et al Neuron. 1991 Aug . 7(2):221-9.

Insert

Dlx2 (Dlx2 Mouse)

Expression

Bacterial

Mutation

Partial cDNA

Available Since

Aug. 20, 2007

Availability

Academic Institutions and Nonprofits only
TurboRFP-pBAD

Plasmid

#54858

Purpose

Localization: Protein Expression Vector , Excitation: 553, Emission: 574

Depositor

Dmitriy Chudakov, Michael Davidson, Dorus Gadella

Article

Merzlyak et al Nat Methods. 2007 Jul;4(7):555-7. Epub 2

Type

Empty backbone

Tags

6xHis and TurboRFP

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pCCL-GKL1RC-Short-LEUg1mKateg2GKL2ORF10UCS

Plasmid

#52345

Purpose

Integration cassette to create p1-Short-Leu-mKate with p2 ORF10 UCS>mKate

Depositor

Chang Liu

Article

Ravikumar et al Nat Chem Biol. 2014 Mar;10(3):175-7. doi

Insert

mKate2

Use

P1 integration vector

Expression

Bacterial

Mutation

changed V2H

Promoter

pGKL2 ORF10 UCS

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT13

Plasmid

#200993

Purpose

pLv1-pUC18R6KT-miniTn7-Tc, TcR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Expression

Bacterial

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTn7-SCOUT12

Plasmid

#200992

Purpose

pLv1-pUC18R6KT-miniTn7-Km, KmR, ApR

Depositor

Philip Poole

Article

Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

Type

Empty backbone

Expression

Bacterial

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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p6272 pDEST-60-GST-HPV16E6

Plasmid

#24127

Depositor

Peter Howley

Insert

HPV16 E6 (E6, E6 HPV)

Expression

Bacterial and Mammalian

Mutation

none

Available Since

April 2, 2010

Availability

Academic Institutions and Nonprofits only
RCAS(B)-Rheb-Q64L

Plasmid

#21050

Depositor

Peter Vogt

Article

Jiang et al Oncogene. 2008 Sep 25. 27(43):5729-40.

Insert

Ras-homolog enriched in brain (RHEB Human)

Use

Retroviral

Tags

FLAG

Expression

Bacterial

Mutation

glutamine 64 to leucine

Available Since

Aug. 28, 2009

Availability

Academic Institutions and Nonprofits only
His SUMO HNRNPA1 dHexa

Plasmid

#197013

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Molliex et al Cell. 2015 Sep 24;163(1):123-33. doi: 10

Insert

HNRNPA1

Tags

6His-SUMO

Expression

Bacterial

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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HPF1-c004

Plasmid

#216874

Purpose

Bacterial expression vector for E. coli producing full length HPF1 protein with N-terminal YFP fusion

Depositor

Lari Lehtiö

Article

Dhakar et al Sci Rep. 2024 Feb 16;14(1):3875. doi: 10

Insert

Full length HPF1 (HPF1 Human)

Expression

Bacterial

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-29b(+)_His-CBD-3C-EmPNGaseF

Plasmid

#218193

Purpose

Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola PNGaseF

Depositor

Florence Pojer

Insert

PNGaseF

Tags

tufA-His-CBD-3C

Expression

Bacterial

Mutation

codon optimized Ecoli, residues 41-354

Promoter

T7

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pGE144 (MutaEco; aTC-induced)

Plasmid

#189824

Purpose

Expresses MutaEco for generating mutations in the E. coli genome (aTC-induced)

Depositor

Seokhee Kim

Article

Eom et al Nucleic Acids Res. 2022 Apr 22;50(7):e38

Insert

EcoRNAPα-PmCDA1

Expression

Bacterial

Promoter

tetR/tetA promoter

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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