31,586 results
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Plasmid#205020PurposeBroad host-range bacterial expression vector with constitutive Pc promoter. Provides E. coli TorA signal peptide in frame with mNeonGreen (codon optimized for expression in P. fluorescens); for targeting mNeonGreen to the periplasmDepositorInsertTorA-mNeonGreen
ExpressionBacterialMutationmNeonGreen is codon optimized for expression in P…PromoterPcAvailable SinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSKB3.MPN348
Plasmid#11437DepositorInsertmethenyltetrahydrofolate synthetase
Tags6x His and TEV cleavage siteExpressionBacterialAvailable SinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-Zeo-dsRedExpress
Plasmid#65040PurposeZeoR mini-Tn7 vector, mobilizable, for RFP tagging bacteriaDepositorInsertdsRedExpress
ExpressionBacterialAvailable SinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-His-VCre
Plasmid#123131PurposeBacterial expression plasmid for wild-type VCre with an N-terminal His-tag.DepositorInsertVCre recombinase
UseCre/LoxTags6x His tagExpressionBacterialPromoterT7Available SinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBRα
Plasmid#53731PurposePlpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.DepositorInsertαNTD
ExpressionBacterialPromoterlpp/lacUV5 (tandem promoter)Available SinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pExodus CMV.Trex2
Plasmid#40210PurposeMammalian expression of codon-optimized Trex2.DepositorInsertThree prime repair exonuclease 2 (Trex2 Mouse)
ExpressionBacterial and MammalianPromoterCMV and T7Available SinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a SUMO Nup53xl 1-320
Plasmid#85998Purposebacterial expression of his6 and SUMO tagged Xenopus laevis Nup53DepositorInsertXenopus laevis Nup53 (nup35.L Frog)
TagsSUMOExpressionBacterialMutationcodon optimzed for bacterial expressionPromoterT7Available SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET tdTomato LIC cloning vector (H6-tdTomato)
Plasmid#86928PurposepET LIC cloning vector for Biotin-His6-tev-yORF-tdTomatoDepositorTypeEmpty backboneTags6xHis, AviTag (biotin), TEV cleavage site, and td…ExpressionBacterialPromoterT7Available SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI
Plasmid#115216PurposeExpresses Pin2Cas13b from a SUMO-tagged bacterial expression constructDepositorInsertPin2Cas13
TagsSUMOExpressionBacterialAvailable SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-rssA
Plasmid#89957PurposeContains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.DepositorInsertrssA gRNA
ExpressionBacterialPromoterpTetAvailable SinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJUMP49-2D(sfGFP)
Plasmid#126967PurposeLevel 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP49-2C(sfGFP)
Plasmid#126966PurposeLevel 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP49-2B(sfGFP)
Plasmid#126965PurposeLevel 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
UbcH5B
Plasmid#60443PurposeExpress E2 UbcH5B in E. ColiDepositorAvailable SinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu
Plasmid#216421PurposeEmpty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.DepositorTypeEmpty backboneUseCRISPR; Expression in plasmodium bergheiTags3xmycExpressionBacterialAvailable SinceApril 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPbU6_hdhfr/yfcu
Plasmid#216422PurposeEmpty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter for traditional CRISPR editing.DepositorTypeEmpty backboneUseCRISPR; Expression in plasmodium bergheiExpressionBacterialAvailable SinceApril 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HisYFP-Sp100
Plasmid#53141PurposeE.coli expression of HisYFP tagged human Sp100 fragmentDepositorInsertSp100A fragment (SP100 Human)
TagsHis-YFPExpressionBacterialMutationfragment (amino acids 71-480); deletion of L75PromoterT7Available SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
VRK1_HUMAN_D0
Plasmid#79684PurposeThis plasmid encodes the kinase domain of VRK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSW039
Plasmid#140035PurposeKO of PCC 11901 psbA2 gene/expresses YFP under control of inducible cLac143 promoterDepositorInsertYellow fluorescent protein
ExpressionBacterialPromotercLac143Available SinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRExT2A-NT-PmNG
Plasmid#232200PurposeEndogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for N-terminal tagging: PAC-3xTy-T2A-3xTy-mNeonGreenDepositorInsertmNeongreen
ExpressionBacterialAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only