30,296 results

1. pET-51b(+)-cpHaloTag154/156

   Plasmid

   #194661

   Purpose

   Protein production of circular permuted HaloTag (cpHaloTag154/156) in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   cpHaloTag154/156
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pELxG4

   Plasmid

   #172570

   Purpose

   Inducible gene expression, pET origin, LuxR activator, PLuxB promoter, GFPmut3, Carbenicillin Resistance Marker.

   Depositor

   Christopher Reisch

   Insert

   LuxR-GFPmut3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLuxB

   Available since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDAI-SceI

   Plasmid

   #32061

   Purpose

   Construction of unmarked gene deletions. Broad host range replicative plasmid expressing the I-SceI homing endonuclease

   Depositor

   Miguel Valvano

   Article

   Flannagan et al Environ Microbiol. 2008 Jun;10(6):1652-6

   Insert

   I-SceI nuclease
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pdhfr

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBBR1_DVS_AF

   Plasmid

   #188950

   Purpose

   Level 2 destination vector containing pBBR1 origin of replication and CIDAR AF scars

   Depositor

   Eric Young

   Article

   Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRelA'

   Plasmid

   #175595

   Purpose

   Tet inducible, KAN Resistant, non labelled synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.

   Depositor

   Greg Bokinsky

   Article

   Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

   Insert

   Catalytic domain of E.coli relA gene.
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Only the catalytic domain of the enzyme is used (…

   Promoter

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmAmetrine1.2

   Plasmid

   #42171

   Depositor

   Robert Campbell

   Article

   Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

   Insert

   mAmetrine1.2
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available since

   Feb. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSETa_ mEos4a

   Plasmid

   #51072

   Purpose

   Photoconverible Fluorescent Protein_ Fixative Resistant

   Depositor

   Loren Looger

   Article

   Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Insert

   mEos4a
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFP.R267

   Plasmid

   #138179

   Purpose

   Expresses the split mCherry reporter interrupted by the gp41-1 intein (in cis)

   Depositor

   Baojun Wang

   Article

   Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:

   Inserts

   araC (araC )
   mCherryN(SGY).gp41-1.(SSS)mCherryC.H6
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   P(araBAD) and iPC

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-6P-1-INTS3-FL

   Plasmid

   #128415

   Purpose

   Express INTS3 full-length in E. coli with a GST tag (N-terminal)

   Depositor

   Yuliang Wu

   Article

   Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

   Insert

   INTS3 (INTS3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   full-length

   Promoter

   tac promoter

   Available since

   Aug. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103_ACTG1_Actin

    Plasmid

    #109989

    Purpose

    Protein expression and purification of ACTG1_Actin

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    ACTG1_Actin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AWP-031

    Plasmid

    #213967

    Purpose

    Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for constitutive CRISPR array expression.

    Depositor

    Alexander Westermann

    Article

    Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121

    Insert

    Cas12a (cas12a Segatella bryantii, Synthetic)
    

    Use

    Tags

    3XFLAG

    Expression

    Bacterial

    Mutation

    D875A to inactivate target nuclease activity

    Promoter

    PcfxA, IPTG inducible

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-RIP

    Plasmid

    #11972

    Depositor

    Jon Ashwell

    Article

    Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

    Insert

    RIP (Ripk1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 13, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pCRII-1.1-2.01 beta

    Plasmid

    #69591

    Purpose

    OT-II TCR beta chain cDNA in pCRII

    Depositor

    Francis R. Carbone

    Article

    Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

    Insert

    TCR beta clone 1.1 (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAT220

    Plasmid

    #134888

    Purpose

    Bacterial expression

    Depositor

    Thomas Howard

    Article

    Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pbacPuroR

    Plasmid

    #26348

    Depositor

    Ben Lehner

    Article

    Semple et al Nat Methods. 2012 Jan 30;9(2):118-9. doi

    Insert

    Bacterial promoter::PuroR::bacterial 3p flank
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MP3

    Plasmid

    #69650

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926 dam
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E. coli PBAD

    Available since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJMP8045

    Plasmid

    #220900

    Purpose

    Tn7 transposase (tnsABCD) expression plasmid for Rhodobacter sphaeroides

    Depositor

    Jason Peters

    Article

    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

    Insert

    tnsABCD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDM026

    Plasmid

    #216808

    Purpose

    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and pyrG selectable marker.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Type

    Empty backbone

    Use

    CRISPR; Fungal expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GST-Hsp90 C(626-732)

    Plasmid

    #22483

    Depositor

    William Sessa

    Article

    Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

    Insert

    Hsp90alpha C domain (HSP90AA2P Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBT13-splitC

    Plasmid

    #122596

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge