30,808 results

1. pET28a-LgBiT-SpyCatcher

   Plasmid

   #223634

   Purpose

   Protein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coli

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   SpyCatcher003 S49C
   

   Tags

   His6, LgBiT

   Expression

   Bacterial

   Available Since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHis-LMW_FGF2

   Plasmid

   #157657

   Purpose

   Expresses FGF2 in E.coli cell

   Depositor

   Byung Il Lee

   Article

   Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

   Insert

   Fibroblast growth factor 2 (FGF2 Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   changed Cystein 211,229 to serine, deleted amino …

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJKR-H-mphR

   Plasmid

   #62559

   Purpose

   Macrolide biosensor. High copy plasmid that expresses GFP proportional to macrolide concentration.

   Depositor

   George Church

   Article

   Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

   Insert

   GFP
   

   Expression

   Bacterial

   Available Since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLA230

   Plasmid

   #11724

   Depositor

   Lawrence Loeb

   Article

   Shinkai et al J Biol Chem. 2001 Dec 14. 276(50):46759-

   Insert

   b-lactamase
   

   Expression

   Bacterial

   Mutation

   GAA for Glu26 changed to TAA (premature stop codo…

   Available Since

   Aug. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQC

   Plasmid

   #221134

   Purpose

   Contains [PibpA-gfpASV] and [PcpxPmcherryASV]OPT genetic modules in the pSEVA631(Spec) backbone, separated by the secG leuU terminator

   Depositor

   Daniel Daley

   Article

   Cumming et al ACS Synth Biol. 2024 Apr 27. doi: 10.102

   Inserts

   [PibpA-gfpASV]
   [PcpxPmcherryASV] OPT
   

   Expression

   Bacterial

   Promoter

   cpxP and ibpA

   Available Since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFW2100

   Plasmid

   #224212

   Purpose

   Bacteroides genomic editing tool; IPTG inducible-Fncpf1

   Depositor

   Ophelia Venturelli

   Article

   Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

   Insert

   Fncpf1
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMJ923

   Plasmid

   #78313

   Purpose

   Expression of His6-MBP-tagged Cas9-NLS-mCherry protein in E. coli

   Depositor

   Martin Jinek

   Article

   Burger et al Development. 2016 Apr 29. pii: dev.13480

   Insert

   SpCas9
   

   Tags

   HA-2xNLS-mCherry-NLS and His6-MBP-TEV

   Expression

   Bacterial

   Mutation

   human codon-optimized gene sequence

   Promoter

   T7

   Available Since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSET-WHaloCaMP1b-EGFP

   Plasmid

   #205305

   Purpose

   Expression of WHaloCaMP1b-EGFP fusion in E. coli

   Depositor

   Eric Schreiter

   Article

   Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

   Insert

   WHaloCaMP1b-EGFP
   

   Expression

   Bacterial

   Available Since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. 6BY9 (EHMT1)

   Plasmid

   #119956

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   EHMT1 (EHMT1 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. CLK1

    Plasmid

    #39149

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CLK1 (CLK1 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQLinkHD-SYNZIP2

    Plasmid

    #80658

    Purpose

    Encodes the synthetic coiled coil peptide SYNZIP2

    Depositor

    Amy Keating

    Article

    Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

    Insert

    SYNZIP2
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQLinkHD-SYNZIP1

    Plasmid

    #80647

    Purpose

    Encodes the synthetic coiled coil peptide SYNZIP1

    Depositor

    Amy Keating

    Article

    Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

    Insert

    SYNZIP1
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. 6CC8 (MBD3)

    Plasmid

    #119959

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    MBD3 (MBD3 Human)
    

    Tags

    GSTtag, 6xHis and Thrombin

    Expression

    Bacterial

    Mutation

    MBD domain (contains residues 1-71)

    Promoter

    T7

    Available Since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. PRDM1

    Plasmid

    #25249

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PRDM1_04 (PRDM1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pMCSG53_N

    Plasmid

    #167255

    Purpose

    Bacterial expression of Nucleocapsid protein (residues 1-419)

    Depositor

    Alexei Savchenko

    Insert

    N protein (residues 1-419) (N SARS-CoV-2)
    

    Tags

    His6-TEV site

    Expression

    Bacterial

    Mutation

    none

    Available Since

    April 27, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. SpyTag003-mClover3

    Plasmid

    #133453

    Purpose

    Expresses SpyTag003-mClover3 (a green fluorescent protein) in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-mClover3
    

    Tags

    GSGSGSGS linker and His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSt-KO

    Plasmid

    #44566

    Purpose

    for generation of knockout of mycobacterial genes

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Expression

    Bacterial

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKD233.7-3

    Plasmid

    #90955

    Purpose

    Expresses S. baltica TtrR under the PltetO-1 promoter, sfGFP under PttrB185-269, and mCherry constitutively

    Depositor

    Jeffrey Tabor

    Article

    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

    Inserts

    Tetrathionate response regulator
    Superfolder GFP
    mCherry
    

    Expression

    Bacterial

    Promoter

    Bba_J23114, PltetO-1, and PttrB185-269

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Fast-GCaMP6f-RS09

    Plasmid

    #67160

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Badura et al Neurophotonics. 2014 Oct;1(2):025008.

    Insert

    Fast-GCaMP6f-RS09
    

    Tags

    6xHis, T7 tag, and Xpress tag

    Expression

    Bacterial

    Mutation

    L414T within GCaMP6f

    Promoter

    T7

    Available Since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC028 - LbfCas13a from Listeriaceae bacterium FSL M6-0635

    Plasmid

    #91918

    Purpose

    Expresses LbfCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LbfCas13a and guide expression cassette
    

    Expression

    Bacterial

    Available Since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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