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pMG36E

Plasmid

#133853

Purpose

Expression vector

Depositor

Jan Kok, Gerard Venema

Publication

van de Guchte et al Appl Environ Microbiol. 1989 Jan;55(1):2

Type

Empty backbone

Expression

Bacterial

Promoter

P32

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPEgRNA

Plasmid

#172716

Purpose

Delivery of guide RNA for prime editing

Depositor

Tilmann Weber

Publication

Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

Insert

gRNA

Expression

Bacterial

Available since

Sept. 17, 2021

Availability

Academic Institutions and Nonprofits only

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6His-MBP-TEV-huLbCpf1

Plasmid

#90096

Purpose

Bacterial expression plasmid for protein purification

Depositor

Feng Zhang

Publication

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huLbCpf1

Tags

3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…

Expression

Bacterial

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pDEST-hisMBP-AsCpf1-EC

Plasmid

#79007

Purpose

Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

Depositor

Jin-Soo Kim

Publication

Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Insert

AsCpf1

Tags

6xHis-MBP-NLS and NLS-HA

Expression

Bacterial

Promoter

tac promoter

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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TOPO mKate2

Plasmid

#68441

Purpose

TOPO construct expressing mKate2 for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mKate2

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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GFP-Dynamin 2 K44A

Plasmid

#22301

Depositor

Pietro De Camilli

Publication

Ochoa et al J Cell Biol. 2000 Jul 24. 150(2):377-89.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Dynamin 2 K44A (Dnm2 Rat)

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

K44A

Available since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles
pGSTag

Plasmid

#21877

Depositor

David Ron

Publication

Ron et al Biotechniques. 1992 Dec . 13(6):866-9.

Type

Empty backbone

Tags

GST

Expression

Bacterial

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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Stat2 pBluescript SK (-)

Plasmid

#8705

Depositor

Jim Darnell

Publication

Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

Insert

Stat2 (STAT2 Human)

Expression

Bacterial

Available since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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spMSP1D1

Plasmid

#173482

Purpose

Expresses spMSP1D1 in Bl21

Depositor

Huan Bao

Publication

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spMSP1D1

Tags

Histag

Expression

Bacterial

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCold CL7-Cas9

Plasmid

#124890

Purpose

obtaining full Cas9 RNP

Depositor

Yi Liu

Publication

Qiao et al Commun Biol. 2019 May 3;2:161. doi: 10.1

Insert

Cas9

Expression

Bacterial

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSm-Nuclease

Plasmid

#132431

Purpose

Expresses Serratia marcescens nuclease in E. coli using T7 promoter

Depositor

Mark Arbing

Insert

Serratia marcescens nuclease (codon optimized)

Tags

None

Expression

Bacterial

Promoter

T7

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pSGKP-km

Plasmid

#117233

Purpose

A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Publication

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCN-EF2132tet

Plasmid

#107191

Purpose

Expresses EF2132 recombinase for performing recombineering in S. aureus

Depositor

Steve Salipante

Publication

Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

Inserts

EF2132
tetR

Expression

Bacterial

Promoter

p23

Available since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pACBSR-hyg

Plasmid

#87830

Purpose

Expresses lambda-red system with hyg selection marker

Depositor

Pep Charusanti

Publication

Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

Insert

hyg

Tags

None

Expression

Bacterial

Mutation

None

Promoter

bla

Available since

Aug. 5, 2017

Availability

Academic Institutions and Nonprofits only

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IL-4 promoter luciferase

Plasmid

#12195

Depositor

Anjana Rao

Publication

Macian et al EMBO J. 2000 Sep 1. 19(17):4783-95.

Insert

IL-4 promoter (Il4 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Bacterial

Available since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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eGFP-luciferase-C1

Plasmid

#163523

Purpose

Expresses both eGFP and firefly luciferase in mammalian cells

Depositor

Thomas Weber

Insert

Firefly luciferase

Use

Luciferase

Tags

eGFP

Expression

Bacterial and Mammalian

Available since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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SpyTag003-MBP

Plasmid

#133450

Purpose

Expresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasm

Depositor

Mark Howarth

Publication

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-MBP

Tags

His6 and Thrombin cleavage site

Expression

Bacterial

Mutation

Peptide tag added to N-terminus of MBP via a GSGE…

Promoter

T7

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET28a-MH6-EsCas13d

Plasmid

#108303

Purpose

Expresses E. coli codon optimized EsCas13d in the pET28a backbone.

Depositor

Arbor Biotechnologies

Publication

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Insert

EsCas13d

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Promoter

lac

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET28a_T7-ARG1

Plasmid

#106473

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli

Depositor

Mikhail Shapiro

Publication

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG1

Expression

Bacterial

Promoter

T7

Available since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pDEST14-SpyCatcher002

Plasmid

#102827

Purpose

Bacterial expression of SpyCatcher002 for covalent coupling to SpyTag002-linked proteins

Depositor

Mark Howarth

Publication

Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

Insert

SpyCatcher002

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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