31,879 results
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Plasmid#124601PurposeExpresses protein A and Tn5 Transposase fusion protein in bacterial cellsDepositorInsertProtein A and hyperactive Tn5 transposase (Tnp) fusion protein
TagsProtein A and Tn5 transposase fusion protein with…ExpressionBacterialPromoterT7Available SinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
bZiPro
Plasmid#86846Purposeexpress NS2B-NS3 protease in E.coliDepositorInsertsNS3 1-177
NS2B45-96
Tagsa hexahistidine tag and a thrombin cleavage siteExpressionBacterialMutationNS2B cofactor region (residues 45–96) and NS3 pro…PromoterT7Available SinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-nCas9n
Plasmid#46757DepositorInsertCas9
UseCRISPRTagsSV40 NLSExpressionBacterialPromoterT3Available SinceAug. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
10xHis-MBP-TEV-S. pyogenes dCas9 M1C D10A C80S H840A C574S
Plasmid#60815PurposedCas9 with single cysteine residue (M1C) for site-specific labellingDepositorInsertdCas9 M1C D10A C80S H840A C574S
TagsHis10 and MBPExpressionBacterialMutationM1C D10A C80S H840A C574SAvailable SinceDec. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJMP1039
Plasmid#119239Purposeexpresses Tn7 transposaseDepositorInserttnsABCD
ExpressionBacterialAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p15A-PLtetO1-tetR-T500
Plasmid#45391DepositorInserttet repressor
ExpressionBacterialPromoterPL-tetO1Available SinceJune 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
3X ERE TATA luc
Plasmid#11354PurposeLuciferase reporter containing three copies of vitellogenin Estrogen Response ElementDepositorInsertERE
UseLuciferaseExpressionBacterialAvailable SinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLR16-Phes*
Plasmid#98783PurposeSuicide vector for allelic exchange in Listeria utilizing an engineered counterselection marker for Listeria monocytogenes based on a mutated phenylalanyl-tRNA synthetase gene (pheS*).DepositorInsertpheS
ExpressionBacterialMutationA309GAvailable SinceJan. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
BirA pLEMO
Plasmid#119818PurposeExpress endogenous biotin ligase in E.coliDepositorInsertBiotin ligase
ExpressionBacterialPromoterRhamnose promoterAvailable SinceApril 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTriExRhoA2G
Plasmid#40176DepositorInsertRhoA Biosensor
TagsHis-MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-sfGFP
Plasmid#133454PurposeExpresses SpyTag003-superfolder GFP in bacterial cytoplasmDepositorInsertSpyTag003-sfGFP
TagsGSGSGS linker and His6ExpressionBacterialPromoterT7Available SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDisCoTune
Plasmid#173713PurposePromotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.DepositorInsertsExpressionBacterialAvailable SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAKTaq
Plasmid#25712PurposeBacterial expression of Taq polymeraseDepositorInsertTaq polymerase
ExpressionBacterialAvailable SinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMVV094
Plasmid#215302PurposeExpresses sfGFP under the Anderson J23119 promoter with a pRO1600 backbone.DepositorInsertsfGFP
TagsHisExpressionBacterialPromoterJ23119Available SinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFP-pBAD
Plasmid#54762PurposeLocalization: Protein Expression Vector , Excitation: 488, Emission: 507DepositorHas ServiceCloning Grade DNATypeEmpty backboneTags6xHis and EGFPExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSL1521 (pSPIN, pSC101* backbone)
Plasmid#160729PurposeSingle-plasmid V. cholerae CAST system. Encodes all proteins, crRNA, donor DNA; non-targeting crRNA has BsaI sites for cloning. Temperature-sensitive pSC101* backbone can be cured by 37 °C incubation.DepositorInsertVchCAST proteins, crRNA, and donor DNA
ExpressionBacterialPromoterJ23119Available SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNCS-Antares
Plasmid#74279PurposeConstitutive expression of Antares in bacteriaDepositorInsertAntares
Tags6xHisExpressionBacterialPromoterT7Available SinceMarch 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-RfxCas13d-His
Plasmid#141322PurposePlasmid for bacterial expression and purification of RfxCas13d proteinDepositorInsertRfx-Cas13d
UseCRISPRTags6xHisExpressionBacterialPromoterT7Available SinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-RNAPII-CTD wt #14-HA
Plasmid#160688PurposeExpression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.DepositorInsertRNA Polymerase II CTD
ExpressionBacterialMutationC terminal domain onlyAvailable SinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-EF1a-TetOn3G
Plasmid#104543PurposePiggyBac vector encoding Tet-On 3G constitutively expressed by EF1a promoterDepositorInsertTet-On 3G
ExpressionBacterial and MammalianPromoterEF1aAvailable SinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only