32,926 results
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Plasmid#179318PurposePlasmid for bacterial expression and purification of SpG-Cas9DepositorInsertHuman codon-optimized Cas9 variant SpG
UseCRISPRTags6xHis and SV40-NLSExpressionBacterialMutationSpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337RAvailable SinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTCF37 HLA-B*35:01
Plasmid#180450PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-B*35:01
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available SinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM33_USP7_MATH
Plasmid#178490PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRB035
Plasmid#181944PurposeaTc-inducible expression of mNeonGreenDepositorInsertmNeonGreen; tetR (AX04_RS23485 Synthetic)
ExpressionBacterialPromotermNG-PLtetO-1; tetR-J23106Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV515
Plasmid#177703PurposePlasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hanseniiDepositorInsertsCas9
Nat
tRNA-sgRNA-tRNA
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTCF120 H-2D(b)
Plasmid#180454PurposeE. coli expression of soluble MHC proteinDepositorInsertH-2D(b)
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available SinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTCF200 Mouse beta-2 microglobulin
Plasmid#180462PurposeE. coli expression of class I MHC light chainDepositorInsertMouse beta-2 microglobulin
TagsBSP41ExpressionBacterialAvailable SinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTns(Ptr)
Plasmid#175576PurposeExpression of CRISPR-associated transposasesDepositorInsertPtrTnsABC
ExpressionBacterialMutationNOAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCasTns(Ptr)-IS186
Plasmid#175581PurposeExpression of CRISPR-associated transposasesDepositorInsertPtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(IS186)
ExpressionBacterialMutationNOAvailable SinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT85_PS-mEos3.2_GentaR
Plasmid#173895PurposeTransposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the Spiralin promoterDepositorInsertmEos3.2
ExpressionBacterialPromoterSpiralinAvailable SinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSIBR004
Plasmid#177667PurposeSIBR Intron 4 (Int4) interrupts the CDS of FnCas12aDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterlacUV5Available SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-hRNF216
Plasmid#167354PurposeExpression of human RNF216 (Triad3A) codon optimized for E. coli and insect cellsDepositorInsertE3 ubiquitin-protein ligase RNF216 (RNF216 Human)
Tags3C protease cleavage site and 6x-His tagExpressionBacterialMutationcodon optimised for expression in E. coliPromoterT7Available SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMU-4
Plasmid#172353PurposeCRISPR gene editing in Streptomyces, codon-optimized Cas9 behind theophylline riboswitch and D4 promoter, with custom gRNADepositorInsertD4-TheoR-cas9
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterD4Available SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
MacroH2A1.1-c002
Plasmid#173087PurposeExpression of recombinant fusion protein in E. coliDepositorInsertMACROH2A1 (MACROH2A1 Human)
TagsmCeruleanExpressionBacterialMutationQ162-N369, codon optimizedAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
APLF-c001
Plasmid#173077PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSIBR003
Plasmid#177666PurposeSIBR Intron 3 (Int3) interrupts the CDS of FnCas12aDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterlacUV5Available SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
TARG1-c002
Plasmid#173108PurposeExpression of recombinant fusion protein in E. coliDepositorInsertTARG1
TagsmCeruleanExpressionBacterialMutationFull length proteinAvailable SinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDonorCm(Ptr)
Plasmid#175577PurposeDNA donor for transpositionDepositorInsertMini-Tn, Cm cassette
ExpressionBacterialMutationNOAvailable SinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
Drp1-EGFP-StrepII
Plasmid#174429PurposeExpresses human Drp1 Isoform 3 with EGFP at C-terminus in bacteriaDepositorInsertDrp1
TagsEGFP-StrepIIExpressionBacterialMutationNonePromoterT7Available SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_FAM98A-Halo
Plasmid#175329PurposeProduction of HaloTag fusion proteinDepositorAvailable SinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only