29,974 results

1. pET-28a_6H-MMLV_RT_D524N-6H

   Plasmid

   #166945

   Purpose

   Bacterial expression of M-MLV reverse transcriptase

   Depositor

   Robert Tjian

   Article

   Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

   Insert

   gag-pol
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   D524N, H8Y

   Promoter

   T7

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMBP-AsCas12a

   Plasmid

   #113430

   Purpose

   Expresses 10xHis-MBP fused to AsCas12a

   Depositor

   Jennifer Doudna

   Article

   Chen et al Science. 2018 Apr 27;360(6387):436-439.

   Insert

   AsCas12a
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-MATR3

   Plasmid

   #32880

   Depositor

   Yossi Shiloh

   Article

   Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

   Insert

   MATR3 Matrin3 (MATR3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGS-21a his6-GST-WTPhi29DNAP

   Plasmid

   #166863

   Purpose

   To produce a dual affinity-tagged Phi29 DNA polymerase

   Depositor

   Stephen Quake, Paul Blainey

   Article

   Blainey et al Nucleic Acids Res. 2011 Mar;39(4):e19. d

   Insert

   Phi29 Polymerase codon-optimized for overexpression in E. coli
   

   Use

   Tags

   GST and his6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKAR2-Br512

   Plasmid

   #161875

   Purpose

   Expresses Br512 polymerase in E.coli

   Depositor

   Andrew Ellington

   Article

   Maranhao et al medRxiv

   Insert

   Modified DNA Polymerase I Large Fragment (Br512)
   

   Use

   Tags

   8x His tag; Villin Headpiece 47 amino acid (HP47)…

   Expression

   Bacterial

   Mutation

   Promoter

   T7, tetPA

   Available since

   Nov. 11, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pFA6a-mCherry-hphMX6

   Plasmid

   #105156

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu

   Article

   Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

   Insert

   mCherry-hphMX6
   

   Use

   Tags

   mCherry

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PET-28a-YTH domain of YTHDF2

   Plasmid

   #228776

   Purpose

   Protein expression plasmid for YTH domain of YTHDF2 in E.Coli.

   Depositor

   Huiqing Zhou

   Article

   Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

   Insert

   YTH domain of YTHDF2 (YTHDF2 Human)
   

   Use

   Tags

   6*His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBAD/His-B-iRFP

   Plasmid

   #31855

   Purpose

   a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

   Depositor

   Vladislav Verkhusha

   Article

   Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

   Insert

   iRFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S13L, A92T, V104I, V114I, E161K, Y193K, F198Y, D2…

   Promoter

   araBAD

   Available since

   Sept. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCold CL7-Cas9

   Plasmid

   #124890

   Purpose

   obtaining full Cas9 RNP

   Depositor

   Yi Liu

   Article

   Qiao et al Commun Biol. 2019 May 3;2:161. doi: 10.1

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKM493

    Plasmid

    #140191

    Purpose

    ORBIT integrating plasmid for C-terminal tagging with cleavable EGFP.

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    TEV-Flag-Gly4-eGFP
    

    Use

    Tags

    Flag + eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    PGroEL

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pL0M-S-mKOκ-EC18154

    Plasmid

    #137077

    Purpose

    Golden Gate Level 0 S module for N-terminal fluorescent protein tagging

    Depositor

    Julian Hibberd

    Article

    Luginbuehl et al Plant Direct. 2020 Feb 11;4(2):e00188. d

    Insert

    mKOκ DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pet28a-His6-Halo-TEV-Nrf2

    Plasmid

    #62455

    Purpose

    Halo-His6 tagged Nrf2 for E.coli expression

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Nrf2 (NFE2L2 Human)
    

    Use

    Tags

    His6-Halo-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. SRC_HUMAN_D0

    Plasmid

    #79700

    Purpose

    This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Markus Seeliger, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    SRC (SRC Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pET21b FAST-PETase

    Plasmid

    #225760

    Purpose

    Expresses codon-optimized FAST-PETase in E. coli with a C-terminal his-tag

    Depositor

    Jason Boock

    Article

    Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

    Insert

    FAST-PETase
    

    Use

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTn Donor

    Plasmid

    #236211

    Purpose

    Arabinose-inducible Tn5 InducTn-seq plasmid

    Depositor

    Matthew Waldor

    Article

    Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

    Insert

    None
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX-2TK-14-3-3 eta GST

    Plasmid

    #13277

    Depositor

    Michael Yaffe

    Article

    Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

    Insert

    14-3-3 eta (YWHAH Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 7, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pSecUAG-Evol2

    Plasmid

    #163148

    Purpose

    Expresses machinery necessary for selenocysteine incorporation at UAG codons

    Depositor

    Dieter Söll

    Article

    Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2

    Inserts

    allo-tRNA UTu2D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Cysteine desulfurase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cysteine 32 switched to Selenocysteine (UAG), Cys…

    Promoter

    EM7, araBAD, native As SelD promoter, and native …

    Available since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAG87 FAST

    Plasmid

    #130719

    Purpose

    Expresses His-tagged FAST (also called YFAST) in bacteria

    Depositor

    Arnaud Gautier

    Article

    Plamont et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    FAST
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-sfGFP

    Plasmid

    #85492

    Purpose

    pET28a expressing codon optimized superfolder GFP with C-terminal His6 tag

    Depositor

    Ryan Mehl

    Article

    Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

    Insert

    superfolder GFP
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pWC2

    Plasmid

    #15960

    Depositor

    Jeff Gelles

    Article

    Subramanian et al J Gen Physiol. 2007 Nov . 130(5):445-55.

    Insert

    Kinesin 1-401 BCCP his tag (Khc E. coli, Fly)
    

    Use

    Tags

    BCCP and His6

    Expression

    Bacterial

    Mutation

    amino acids 1-401

    Promoter

    Available since

    Nov. 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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