30,808 results

1. pBS-AMVpl-β1WT-nAchR

   Plasmid

   #97080

   Purpose

   To determine if structural domains are important for nicotinic acetylcholine receptor (nAChr) channel function via dose-response curves from voltage-clamped oocytes to estimate EC50's and Hill Co

   Depositor

   Henry Lester

   Article

   Labarca et al Nature. 1995 Aug 10. 376(6540):514-6.

   Insert

   Beta1 nicotinic acetylcholine receptor (Chrnb1 Mouse)
   

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   July 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEVA221rAPOBEC1-T7RNAP

   Plasmid

   #167979

   Purpose

   Expresses fusion protein rAPOBEC1-T7RNAP in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

   Insert

   rAPOBEC1-T7RNAP (T7p07 Synthetic)
   

   Tags

   T7 tag and T7RNAP

   Expression

   Bacterial

   Promoter

   TetR-Ptet

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKERep

   Plasmid

   #211826

   Purpose

   Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

   Insert

   eGFP
   

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKESa

   Plasmid

   #211828

   Purpose

   Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

   Insert

   eGFP
   

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDUET-1-alpha-synuclein-mCerulean3-His6

   Plasmid

   #110061

   Purpose

   For bacterial expression of human alpha-synuclein, carboxyl terminally tagged with mCerulean3 and poly-histidines

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

   Insert

   alpha-synuclein (SNCA Human)
   

   Tags

   6 x His and mCerulean3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHS0923 pET45b(+) His14-MBP-TEV-AmaTnpB

   Plasmid

   #176587

   Purpose

   Bacterial expression of His14-MBP-TEV-AmaTnpB

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Inserts

   MBP tag
   TnpB
   

   Tags

   His14 tag

   Expression

   Bacterial

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCVD364

   Plasmid

   #63739

   Purpose

   V cholerae 395 nanH gene cloned into the BamHI site of pCVD315

   Depositor

   James Kaper

   Article

   Vimr et al J Bacteriol. 1988 Apr;170(4):1495-504.

   Insert

   nanH
   

   Expression

   Bacterial

   Available Since

   April 6, 2015

   Availability

   Academic Institutions and Nonprofits only

8. GST-PRMT6

   Plasmid

   #34700

   Depositor

   Steven Clarke

   Article

   Frankel et al J Biol Chem. 2002 Feb 1;277(5):3537-43.

   Insert

   PMRT6
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pC0062 RanCas13 (B06) His6-TwinStrep-SUMO-BsaI

   Plasmid

   #115213

   Purpose

   Expresses RanCas13b from a SUMO-tagged bacterial expression construct

   Depositor

   Feng Zhang

   Article

   Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

   Insert

   RanCas13
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   Sept. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCascade_SPS1(PmcCAST)

    Plasmid

    #224926

    Purpose

    Expression and purification of type I-B2 Cascade in CRISPR-associated transposon of Romsetta

    Depositor

    Leifu Chang

    Article

    Wang et al Methods Enzymol. 2025;712:55-79. doi: 10

    Inserts

    Cas5, Cas6, Cas7, Cas8, Cas11
    CRISPR assay for crRNA expression
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET_StrepII_TEV_LIC_TniQ(PmcCAST)

    Plasmid

    #224927

    Purpose

    TniQ expression plasmid for the co-purification of TnsC-TniQ complex

    Depositor

    Leifu Chang

    Article

    Wang et al Methods Enzymol. 2025;712:55-79. doi: 10

    Insert

    TniQ
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXD80RFP-pXD68Kan2-RFP

    Plasmid

    #225329

    Purpose

    RSF1010-based broad host-range plasmid engineered from pXD68Kan2 encoding RFP, kanamycin resistance

    Depositor

    Emily Balskus

    Article

    Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

    Insert

    RFP
    

    Expression

    Bacterial

    Available Since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. QUAS-5-T7-TetO-GFP_ColE1

    Plasmid

    #171660

    Purpose

    QUAS five base pairs upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli according to the Q and Tet systems. Contains the ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    QUAS-5-T7-TetO-GFP ssra-T7 Stop
    

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. T7-LacO-QF-T7-TetO-TetR_p15A

    Plasmid

    #171661

    Purpose

    Expresses QF and/or TetR in BL21(DE3) E. coli according to the presence of aTc. There is no T7 terminator sequence between the QF- and TetR-containing circuits. Contains a p15A origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    QF-T7-TetO-TetR
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX6p-1-FRB-PafA

    Plasmid

    #113404

    Purpose

    Expresses FRB-PafA in bacteria

    Depositor

    Min Zhuang

    Article

    Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

    Insert

    FRB-PafA
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEMJ_042

    Plasmid

    #214061

    Purpose

    T7 inducible LanTERN (pRSET)

    Depositor

    Pamela Silver

    Article

    Jones et al ACS Synth Biol. 2024 Feb 20. doi: 10.102

    Insert

    LanTERN
    

    Tags

    6x His, T7 Tag, and Xpress(tm) Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28-MHL: nsp16_SARS-CoV-2

    Plasmid

    #167850

    Purpose

    Bacterial expression of the SARS-CoV-2 protein nsp16

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp16
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 16, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pBSV2G_2

    Plasmid

    #118225

    Purpose

    Empty E. coli - B. burgdorferi shuttle vector, gentamicin resistant

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHCKan-yibDp2-GFPuv

    Plasmid

    #139083

    Purpose

    Low phosphate inducible promoter yibDp2 for GFP expression

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2020 Jun 19;9(6):1483-14

    Insert

    yibDp2-GFPuv
    

    Expression

    Bacterial

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. zCD2

    Plasmid

    #122031

    Purpose

    Expresses histone deacetylase 6 (HDAC6) catalytic domain 2 (CD2), also known as zCD2, in Escherichia coli cells.

    Depositor

    David Christianson

    Article

    Hai et al Nat Chem Biol. 2016 Sep;12(9):741-7. doi

    Insert

    Zebrafish HDAC6 Catalytic Domain 2
    

    Tags

    His6-MBP tag with cleavable TEV site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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