31,059 results
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Plasmid#208737PurposeCharacterization plasmid for PCap (sfgfp expressed from PCap on the S. aureus pC194 replicon)DepositorInsertPCap
UseSynthetic BiologyExpressionBacterialPromoterPCapAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-5'TwStrep
Plasmid#208620PurposeEmpty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.DepositorTypeEmpty backboneTagsTwin StrepExpressionBacterialPromoterT7Available SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMM660
Plasmid#68889PurposeArabinogalactan-inducible NanoLuc expression, pNBU2 backbone, AmpRDepositorInsertNanoLuc
ExpressionBacterialAvailable SinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBL6
Plasmid#50549PurposeExpresses mCherry under the PLtetO-1 promoterDepositorInsertmCherry
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
EV-D68 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag
Plasmid#204817PurposeBacterial expresison of Enterovirus D68 3C proteaseDepositorInsertEV D68 3C protease
ExpressionBacterialAvailable SinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
5KO9 (HMCES)
Plasmid#101877PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHMCES (HMCES Human)
Tags6x His (modified C-term tag is AENLYFQSHHHHHH)ExpressionBacterialPromoterT7Available SinceApril 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
SW5
Plasmid#40108DepositorInsertBistable switch using dynamic degradation
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pOGG043
Plasmid#133123PurposeLevel 0 PU module synthesised as a fragment (pL0M-PU-PsNifH). Synthetic consensus promoter to drive nodule-specific expression in biovars and strains of R. leguminosarum.DepositorInsertPsNifH Synthetic consensus nifH promoter
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT2/SVNeo
Plasmid#26553DepositorInsertpT2/SVNeo
ExpressionBacterialMutationadded the Rmut(80, 87, d101)Available SinceMarch 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAB152
Plasmid#101663PurposeOpto-T7RNAP*(563-F2)DepositorInsertOpto-T7RNAP*(563-F2)
UseSynthetic BiologyExpressionBacterialAvailable SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUB-MSP1D1
Plasmid#161609PurposeBacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formationDepositorInsertUD-MSP1D1
Tags7-His tagExpressionBacterialMutationmutant of MSP1D1 ( Q138W, L181W, Y236W)PromoterT7Available SinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
BASIC_8_RBS34- mCherry -B0015
Plasmid#68142Purposestorage vector for BASIC part: RBS-RFP(ORF)DepositorInsertRBS34-mcherry-B0015
ExpressionBacterialAvailable SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSW149
Plasmid#122248PurposepTYB2 containing TIF4631 gene, expresses yeast eIF4G1 as an intein-chitin-binding domain fusion for purification of untagged full-length protein, with or without a fluorescent label on the C-terminus.DepositorInsertTIF4631 (TIF4631 Budding Yeast)
TagsIntein-Chitin-binding domainExpressionBacterialPromoterT7Available SinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAT224
Plasmid#134892PurposeBacterial expressionDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMEG_YLCas9_empty
Plasmid#117826PurposeCRISPR/Cas9 plasmid for GoldenMOCS in Y. lipolyticaDepositorInsertYlpTEF_hCas9_YlCyc1TT
ExpressionBacterial and YeastAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMMB66EH-aphA
Plasmid#44392DepositorInsertAphA
ExpressionBacterialPromotertacAvailable SinceApril 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern/pRSETB
Plasmid#51969Purposebacterial expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imagingDepositorInsertNano-lantern
Tags6xHisExpressionBacterialMutationS257G in Rluc8Available SinceMarch 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDR111_mannose_Bgal no NLS
Plasmid#188399PurposeExpresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.DepositorInsertlacZ
TagsPhoD secretion peptideExpressionBacterialPromoterD-mannoseAvailable SinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
APneg-SNAP25a
Plasmid#168011Purposenegative selection PACE Accessory PlasmidDepositorInsertSNAP25-T7PAP
ExpressionBacterialAvailable SinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYTK034
Plasmid#65141PurposeEncodes mRuby2 as a Type 3 part to be used in the Dueber YTK systemDepositorInsertmRuby2
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only