31,587 results
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Plasmid#119091PurposeBacterial expression of human phox homology (PX) domain, SNX10-PX (1-201)DepositorAvailable SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only
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pDE43-MCSq17
Plasmid#27333DepositorInsertqtag17
ExpressionBacterialMutationN/AAvailable SinceMay 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase-NLS-cTF
Plasmid#87746PurposeIPTG-inducible expression of ligase-NLS-cTF fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
Tags6xHis and NLS linker - cTF (chimeric transcriptio…ExpressionBacterialPromoterT5-lacAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
N-WASP
Plasmid#186132PurposeFor expression of recombinant N-WASp in bacteria cells.DepositorInsertWASL (WASL Rat)
TagsHis6ExpressionBacterialMutationAmino Acid residues 183-193 fused to 273-501PromoterT7Available SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 IQGAP1 WW domain
Plasmid#104279PurposeBacterial expression of WW domain from IQGAP1DepositorInsertIQGAP1 WW domain (IQGAP1 Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-20b-M-Ntx-S1
Plasmid#58716PurposeExpresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1SDepositorInsertNotexin
TagsMethionineExpressionBacterialMutationChanged Asparagine 1 to SerinePromoterT7Available SinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJKR-H-araC
Plasmid#62563PurposeArabinose biosensor. High copy plasmid that expresses GFP proportional to arabinose concentration.DepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX Socs1-SH2
Plasmid#46504DepositorInsertSocs1 (SOCS1 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-166PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET3a-Yuh1
Plasmid#18895DepositorInsertUbiquitin C-terminal hydrolase I (YUH1 Budding Yeast)
ExpressionBacterialAvailable SinceSept. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-RIP
Plasmid#11972DepositorAvailable SinceJuly 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSL0828 (pQCascade_crRNA-4)
Plasmid#130637PurposeExpresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)
UseCRISPR; TransposonExpressionBacterialPromoterT7Available SinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GGG-ELP(40nm)-Cys
Plasmid#91571PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal CysteineDepositorInsertGGG-ELP(40nm)-Cys
TagsCysteine and Sortase-TagExpressionBacterialPromoterT7Available SinceFeb. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB
Plasmid#216233PurposeS. aureus USA300 GyrB Codon-optimized for E. coliDepositorInsertDNA gyrase
Tags6xHisExpressionBacterialPromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
SUMO_TwinStrep_Cas7-11_crRNA
Plasmid#197612PurposeFor protein purification of Cas7-11DepositorInsertSUMO_TwinStrep_Cas7-11 with crRNA
ExpressionBacterialAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMTTH+bromo5
Plasmid#157803PurposeExpression of synthetic protein composed of 5 repeats of BRD4 bromodomain #1DepositorInsertbromo5
TagsHis6 and MBPExpressionBacterialPromoterpTACAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDYU1B/mRuby3–α-tubulin
Plasmid#154302PurposeExpresses mRuby3–α-tubulin from actin15 promoter in Dictyostelium discoideumDepositorInsertalpha-tubuin
UseDictyostelium expressionTagsmRuby3ExpressionBacterialAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNP1 sfGFP toehold switch
Plasmid#107355PurposeExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 sfGFP trigger is co-expressedDepositorInserttoehold 7 + sfGFP
ExpressionBacterialPromoterT7Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFCM1
Plasmid#64948Purposesource of CmR FRT cassetteDepositorInsertFRT-cat-FRT
ExpressionBacterialAvailable SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X2-RalBP1
Plasmid#37245DepositorAvailable SinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA-32Asp8
Plasmid#102617PurposeExpression of human RPA with aspartic acid substituted phosphorylation domainDepositorExpressionBacterialMutationwith aspartic acid substituted phosphorylation do…Available SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only