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Plasmid#69835PurposeBacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.DepositorInsertSpyCatcher-ELP-GFP
UseTags6x His Tag, GFP, and TEV TagExpressionBacterialMutationPromoterT5 promoter/lac operator elementAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T SHP1 WT
Plasmid#8594DepositorInsertSHP1 (PTPN6 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET22b 6His SpyCatcher-GRFT
Plasmid#204502PurposeBacterial expression of GRFT with N-terminal SpyCatcher fusion and GS linkerDepositorInsertGRFT
UseSynthetic BiologyTags6xHis and SpyCatcherExpressionBacterialMutationPromoterT7Available sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ESRRG_Hormone-recep
Plasmid#109910PurposeProtein expression and purification of ESRRG_Hormone-recepDepositorInsertESRRG_Hormone-recep (ESRRG Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKEW302-AcrVA4
Plasmid#115656PurposeExpresses AcrVA4 in bacteria, under TetR control.DepositorInsertAcrVA4
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCoofy5
Plasmid#43989DepositorTypeEmpty backboneUseTagsHis6-Sumo1ExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_WT
Plasmid#98657PurposeExpresses 6xHis-tagged hnRNPA2 AA 190-341DepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
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R619-X67-527: His6-SARS-CoV-2 N
Plasmid#170204PurposeE. coli expression of SARS-CoV-2 nucleocapsid proteinDepositorInsertSARS-CoV-2 N (N SARS-CoV-2)
UseTagsExpressionBacterialMutationPromoterT7Available sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3(D3A: D175A D28A D9A)
Plasmid#90090PurposeExpresses caspase 3 D3A mutant (D175A D28A D9A) in bacterial cellsDepositorInsertCaspase 3 (CASP3 Human)
UseTagsHis tagExpressionBacterialMutationD175A D28A D9APromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2G1
Plasmid#15790DepositorInsertUbiquitin conjugating enzyme UbE2G1 (UBE2G1 Human)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
ETV2_pet28a
Plasmid#131665PurposeBacterial expression of ETS gene ETV2DepositorInsertETV2 (ETV2 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAD16_CAD_12-490_pvp008
Plasmid#124842PurposeExpresses Aspergillus terreus CAD in E.coliDepositorInsertcis-aconitate decarboxylase
UseTagsStrepTag and TEV siteExpressionBacterialMutationdeleted amino acids 1-11, synthetic codon optimiz…PromoterT7Available sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJCC_076 AsCas12a R1226A
Plasmid#171669PurposeFor bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a R1226A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged arginine 1226 to alaninePromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBB530
Plasmid#27391DepositorInsertgrpE
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
HC25 pComb3xss anti-Homer1 nanobody
Plasmid#134718PurposeBacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-Homer1 (Mouse) recombinant llama nanobody HC25 (Homer1 L. glama (llama))
UseTags6xHis-HAExpressionBacterialMutationPromoterlacAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCoofy31
Plasmid#43993DepositorTypeEmpty backboneUseTagsHis6 and OneStrepExpressionBacterialMutationPromoterT7Available sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCH1 - pBait-empty
Plasmid#174663Purposearabinose-induced synthesis of an RNA containing a single MS2 hairpin. Used as a three-hybrid control. For cloning purposes, see plasmid #174662.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWJ68
Plasmid#46571PurposeSame as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)DepositorInsertstracrRNA
w-dcas9
CRISPR array
UseCRISPRTagsrpoZExpressionBacterialMutationD10A, H840APromoterAvailable sinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -