32,417 results
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Plasmid#129552PurposeContains codon-optimized Sth1Cas9 and gRNA for streptomycesDepositorInsertSth1Cas9
ExpressionBacterialPromoterrpsL(XC)-BbsIAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSpyC-GFP
Plasmid#220191PurposesgRNA expression vector - pBG42 promoter with GFP in sgRNA siteDepositorInsertpBG42 promoter with GFP in sgRNA site
UseCRISPRExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG42Available SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-LacI-dCas9
Plasmid#220194Purposemini-Tn7 dCas9 expression vector - LacI/pLac promoterDepositorInsertPseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
UseCRISPRExpressionBacterialMutationPseudomonas aeruginosa codon optimizedPromoterLacI/pLacAvailable SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD
Plasmid#13668DepositorInsertGateway cassette
UseCo-expressionTagsHisExpressionBacterialAvailable SinceApril 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET28a Gx-NL
Plasmid#169105PurposeExpresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - NanoLuc
Tagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG017 (AVAST type 1)
Plasmid#157895PurposeExpresses the AVAST type 1 defense systemDepositorInsertAVAST type 1 (STAND ATPase with N-terminal serine protease)
ExpressionBacterialAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
4QQG
Plasmid#61948PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHTATIP:JMC001-B06:C41570 (KAT5 Human)
Tags6xHis and MHHHHHHSSGRENLYFQGExpressionBacterialMutationSee commentsPromoterT7Available SinceMarch 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJC175e-DddI
Plasmid#179693PurposeMutagenesis plasmid with constitutive bacterial expression of DddI immunity proteinDepositorInsert[SD8] gIII
ExpressionBacterialAvailable SinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBsVchCAST-pyrE
Plasmid#203813PurposeExpression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coliDepositorInsertVchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(pyrE)
UseCRISPRExpressionBacterialAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-Zn2
Plasmid#174805PurposeBacterial expression of isolated PARP1 Zn2 domainDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-Zn3
Plasmid#174806PurposeBacterial expression of isolated PARP1 Zn3 domainDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEB1-moxCerulean3
Plasmid#103975PurposePlasmid encoding moxCerulean3DepositorInsertmoxCerulean3
UseLow copyExpressionBacterialMutationMutations relative to wild-type GFP (F64L, Y66W, …PromoterproCAvailable SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEL1092
Plasmid#195635PurposeExpression/purification of 6xHis-TEV-sfGFPDepositorInsertsfGFP
ExpressionBacterialAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMR-A-3-Sasg
Plasmid#214349PurposeExpresses T7-RNAP, and cloning backbone for sgRNADepositorInsertT7-RNAP
ExpressionBacterialAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_HECT
Plasmid#187719PurposeExpress GST-tagged NEDD4L HECT domain in bacterial cellsDepositorInsertNEDD4L residues 594-975 (NEDD4L Human)
TagsGST-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-593Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
MP6-SlugABEN-738
Plasmid#214348PurposeExpresses N-terminus of ABE, and untargeted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA emrR ugi CDA1 and N-terminus of ABE
ExpressionBacterialAvailable SinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBT120a17-sg-del
Plasmid#214347PurposeExpresses pIIIDepositorInsertgIII-xLuxAB
ExpressionBacterialAvailable SinceFeb. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
TJP2_PDZ_3
Plasmid#103949PurposeProtein expression and purification of X104 PDZ3 domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
TJP2_PDZ_2
Plasmid#103955PurposeProtein expression and purification of X104 PDZ2 domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
LARS_pNIC-Bio3
Plasmid#153052PurposeExpression construct for producing Avi tagged Leucyl-tRNA synthetaseDepositorInsertLARS (LARS1 Human)
TagsAvi and His6-TEVExpressionBacterialMutationNot full length proteinPromoterT7Available SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only