30,808 results

1. pEN-L1-PJ23119-PplpT-sfGFP-TrrfB-BsaI-Scaf-L2

   Plasmid

   #196250

   Purpose

   Plasmid for cloning of a CRISPR-Cas9 guide RNA gene under promoter PJ23119

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   attL1-GlpT-scGFP-attL2
   

   Expression

   Bacterial

   Promoter

   J23119 promoter

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET 15b-UbcH5a/2417

   Plasmid

   #11472

   Depositor

   Allan Weissman

   Article

   Jensen et al J Biol Chem. 1995 Dec 22. 270(51):30408-

   Insert

   UbcH5a (UBE2D1 Human)
   

   Expression

   Bacterial

   Mutation

   n/a

   Available Since

   April 5, 2006

   Availability

   Academic Institutions and Nonprofits only

3. 6xHis-MBP-huBoCas12a

   Plasmid

   #174673

   Purpose

   Expresses 6xHis-MBP tagged BoCas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huBoCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD-T-GECO1

   Plasmid

   #191616

   Purpose

   Bacterial expression plasmids for Teal Fluorescent Genetically Encoded Calcium Indicator T-GECO1

   Depositor

   Robert Campbell

   Article

   Aggarwal et al Neurophotonics. 2024 Apr;11(2):024207. d

   Insert

   T-GECO1
   

   Tags

   His tag

   Expression

   Bacterial

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPBC-G6f

   Plasmid

   #62811

   Purpose

   pPBC-G6f expresses PiggyBac inverted terminal repeat-flanked GCaMP6f under the control of the CAG promoter.

   Depositor

   Karen Wilcox

   Article

   Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

   Insert

   ITR-CAG-GCaMP6f-ITR
   

   Expression

   Bacterial and Mammalian

   Promoter

   CAG

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQTEV-FLJ30046

   Plasmid

   #34828

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   FLJ30046 (SLAIN1 Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFKM1

   Plasmid

   #64950

   Purpose

   source of KmR FRT cassette

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Methods. 2005 Jun;2(6):443-8.

   Insert

   FRT-KmR-FRT
   

   Expression

   Bacterial

   Available Since

   Nov. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7-L1T1L-NLuc

   Plasmid

   #128942

   Purpose

   Expresses L1-NLuc gene

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al J Virol. 2019 May 8. pii: JVI.00401-19.

   Insert

   L1 minor core protein
   

   Tags

   Nanoluc

   Expression

   Bacterial

   Promoter

   T7 promotor

   Available Since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMUT2-pLac-mCherry

   Plasmid

   #192859

   Purpose

   pMUT2 based vector for sensing lactic acid in E. coli Nissle 1917

   Depositor

   Morten Sommer

   Article

   Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

   Insert

   lldR operon
   

   Expression

   Bacterial

   Available Since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST-N2H-N1

    Plasmid

    #125547

    Purpose

    Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment1

    Depositor

    Marc Vidal

    Article

    Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

    Type

    Empty backbone

    Use

    In vitro expression

    Tags

    NanoLuc fragment1 attached to n-terminus of Gatew…

    Expression

    Bacterial and Mammalian

    Promoter

    synthetic promoter containing yeast, mammalian, a…

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. poRFP-oGFP

    Plasmid

    #154130

    Purpose

    Expresses orthogonal gfp and orthogonal rfp genes in bacterial cells

    Depositor

    Alexander Mankin

    Article

    Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

    Inserts

    orthogonal gfp
    orthogonal rfp
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMRE-Tn7-130

    Plasmid

    #118548

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    TagBFP2
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. 6BHD (SETDB1)

    Plasmid

    #119955

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    SETDB1 (SETDB1 Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    contains amino acids 190-410

    Promoter

    T7

    Available Since

    Feb. 1, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pH6HTC_HNRNPA1-Halo

    Plasmid

    #175307

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    HNRNPA1 (HNRNPA1 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. PA-DAD-wt

    Plasmid

    #53095

    Purpose

    Expresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.

    Depositor

    Klaus Hahn, Ronen Zaidel-Bar

    Article

    Rao et al Cytoskeleton (Hoboken). 2013 Jul;70(7):3

    Insert

    LOV(wt)-6aa-DAD
    

    Tags

    6x His and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    L47F and C49S in Venus

    Available Since

    Aug. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBbE5a-RFP

    Plasmid

    #35277

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    lacUV5

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSC-PreE2

    Plasmid

    #52264

    Purpose

    Bacterial expression of GST-tagged TARGET proteins fused to SUMO-E2 with PreScission protease cleavage site linker

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    Ubc9 GST fusion (UBE2I Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid

    #223065

    Purpose

    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    

    Use

    CRISPR

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Bacterial

    Mutation

    Three regions of SpCas9 encode type IIS restricti…

    Promoter

    Dual T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRExT2A-CT-PmSc

    Plasmid

    #232549

    Purpose

    Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mScarlet-3xTy-T2A-PAC-3xTy

    Depositor

    Calvin Tiengwe

    Article

    Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

    Insert

    mScarlet
    

    Expression

    Bacterial

    Mutation

    None

    Available Since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pST39-Hrs-STAM1 core

    Plasmid

    #21497

    Depositor

    James Hurley

    Article

    Ren et al Structure. 2009 Mar 11. 17(3):406-16.

    Insert

    pST39- Hrs(398-514)-His/STAM1(301-387) (STAM, HGS Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    none

    Available Since

    Feb. 1, 2010

    Availability

    Academic Institutions and Nonprofits only