30,320 results

1. 5VC9 (CXXC4)

   Plasmid

   #101263

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CXXC4 (CXXC4 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 31, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pET20b_Pa-PiuA

   Plasmid

   #128955

   Purpose

   expresses Pseudomonas aeruginosa PiuA in E. coli

   Depositor

   James Naismith

   Article

   Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

   Insert

   iron transport outer membrane receptor (PiuA) (PA4514 Pseudomonas aeruginosa PAO1)
   

   Use

   Tags

   6xHis and pelB leader

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed

   Promoter

   T7

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMLBAD-eGFP

   Plasmid

   #112918

   Purpose

   Fluorescence microscopy

   Depositor

   Miguel Valvano

   Article

   Lefebre et al Appl Environ Microbiol. 2002 Dec;68(12):

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pbad

   Available since

   Aug. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGST-Vps4

   Plasmid

   #21495

   Depositor

   James Hurley

   Article

   Wollert et al Nature. 2009 Mar 12. 458(7235):172-7.

   Insert

   VPS4 (VPS4 Budding Yeast)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Sept. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. ARH1-c003

   Plasmid

   #173093

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   ADPRH (ADPRH Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   E2-L357, codon optimized

   Promoter

   Available since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD-jRCaMP1a

   Plasmid

   #113936

   Purpose

   Bacterial expression of red fluorescent Ca2+ sensor jRCaMP1a

   Depositor

   Robert Campbell, Thomas Hughes

   Article

   Molina et al Biophys J. 2019 May 21;116(10):1873-1886

   Insert

   jRCaMP1a
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCoofy3

   Plasmid

   #43983

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Use

   Tags

   His6-GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSFDuet-1_VNp-Uricase

   Plasmid

   #182401

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-uricase fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-uricase
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBP-C_ccdB

   Plasmid

   #204114

   Purpose

   Type 0 CDS part

   Depositor

   Mikhail Shapiro

   Insert

   Toxic DNA gyrase inhibitor ccdB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pB1H2 UV5 Zif268-5ala (2-hybrid bait plasmid)

    Plasmid

    #128165

    Purpose

    UV5 promoter drives expression of Zif268-5alanine peptide fusion. Pair with pGHUC w-ERBIN as a negative control (doesn't turn on HIS3/GFP).

    Depositor

    Marcus Noyes

    Article

    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928

    Insert

    10 amino acid glycine/serine linker + 5alanines
    

    Use

    Synthetic Biology

    Tags

    FLAG and zif268

    Expression

    Bacterial

    Mutation

    Promoter

    UV5

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRE-Tn7-167

    Plasmid

    #118579

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mClover3
    

    Use

    Minitn7 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. GST-NDP52

    Plasmid

    #171422

    Purpose

    For the purifcation of NDP52 protein

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET His6 Sumo TEV expression vector with BioBrick polypromoter restriction sites (14-S)

    Plasmid

    #48313

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6 and Sumo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJB153

    Plasmid

    #92328

    Purpose

    Express mEos3.2 tagged ZapA in E coli

    Depositor

    Jie Xiao

    Article

    Lyu et al Biopolymers. 2016 Oct;105(10):725-34. do

    Insert

    ZapA
    

    Use

    Tags

    mEos3.2

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Ki67 (LR)-mNeonGreen-IRESpuro2

    Plasmid

    #183743

    Purpose

    Expresses Ki67 (LR)-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 LR domain (MKI67 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    LR domain of Ki67

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. p44K

    Plasmid

    #45800

    Depositor

    Luc Ponchon

    Article

    Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET mCitrine LIC cloning vector (u-mCitrine)

    Plasmid

    #29771

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    mCitrine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEST14-ARL3

    Plasmid

    #67376

    Purpose

    Expression of native hArl3 in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL3 (ARL3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

19. SETD1A

    Plasmid

    #32868

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SETD1A (SETD1A Human)
    

    Use

    Tags

    His tag (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

20. Mut2-RS

    Plasmid

    #182882

    Purpose

    A pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut2-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.

    Depositor

    Miriam Amiram

    Article

    Hadar et al Chembiochem. 2021 Apr 16;22(8):1379-1384

    Insert

    Mut2-RS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge