32,457 results
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Plasmid#31190PurposetRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.DepositorInsertM.j. p-benzoylphenylalanine RS (2 copies +tRNA)
ExpressionBacterialMutationY32G, E107P, D158T, I159SAvailable SinceDec. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
1GFP/RNase H1 D210N
Plasmid#174448PurposeExpress GFP-tagged RNase H1 D210N in E. coli.DepositorInsertRNase H1 (RNASEH1 Human)
TagsHis-tag, GFPExpressionBacterialMutationD210NPromoterT7 promoterAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_NSP4ss_SerM_NucA-His
Plasmid#218190PurposeBacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)DepositorInsertnucA
Tags10xHis and NSP4 signal secretion peptideExpressionBacterialMutationcodon optimized for E. coli, residues 22-266PromoterT7Available SinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
repressilator (pZS1-lTlrLLtCL)
Plasmid#26489DepositorInsertLacI, tetR
ExpressionBacterialAvailable SinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLPT234
Plasmid#127855PurposeRepressilator 2.0, without degradation tags, integrated triple reporterDepositorInsertsbla
mSCFP3
mKate2
mVenus
TetR
LacI
cI
UseSynthetic BiologyExpressionBacterialMutationFirst 11aa derived from mCherry as fusion proteinPromoterpAmpR, pL LacO1, pL tetO1, and pRAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSETa mEos2
Plasmid#20341Purposebacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)DepositorInsertmEos-2
TagsHisExpressionBacterialAvailable SinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt
Plasmid#85111Purposeexpression of recombinant human mature (delta19) ETFalpha wt without tagDepositorAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pF709-pET28a-Hs-FL-ETFbeta-NHis wt
Plasmid#85110Purposeexpression of recombinant human full-length ETFbeta wt with N-terminal 6xHis-tagDepositorAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAM552
Plasmid#154131PurposeExpresses wt rRNAs operon in bacterial cellsDepositorInsertwt bacterial rRNAs operon under control of pL promoter
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
ptRNA100
Plasmid#154133PurposeExpresses a set of tRNAs in bacterial cellsDepositorInserttRNAs operon under control of Ptac promoter
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only