30,327 results

1. pDipA-TEV-His12

   Plasmid

   #137042

   Purpose

   expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi DipA with C-terminal TEV-protease-cleavable His12

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66790.1 (BB_0418 Borrelia burgdorferi B31)
   

   Use

   Tags

   His12 (TEV protease cleavable)

   Expression

   Bacterial

   Mutation

   wild type, full-length protein, contains signal p…

   Promoter

   T7

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTKhc-yibDp-GFP-cp6

   Plasmid

   #134938

   Purpose

   Low phosphate inducible circular permutation of GFP

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   GFPcp6
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TRBV24-1

   Plasmid

   #123455

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRBV24-1 (TRBV24-1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TRBV6-2

   Plasmid

   #123422

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRBV6-2 (TRBV6-2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TRBV4-3

   Plasmid

   #123413

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRBV4-3 (TRBV4-3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TRAV39

   Plasmid

   #123406

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRAV39 (TRAV39 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TRAV10

   Plasmid

   #123382

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRAV10 (TRAV10 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TRAV9-2

   Plasmid

   #123381

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRAV9-2 (TRAV9-2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TRAV8-6

   Plasmid

   #123378

   Purpose

   construction of TCR

   Depositor

   Catherine Wu

   Article

   Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

   Insert

   TRAV8-6 (TRAV8-6 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Wild-type CoV-2 ORF8

    Plasmid

    #159933

    Purpose

    Protein overexpression in E.coli

    Depositor

    James Hurley

    Article

    Flower et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

    Insert

    CoV-2 ORF8 18-121 (ORF8 CoV-2)
    

    Use

    Tags

    6x Histidine, TEV cleavable

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNS38-SadCas9-SNAP

    Plasmid

    #113718

    Purpose

    Bacterial vector for expression of Snap-tagged Staphylococcus aureus dCas9

    Depositor

    Gerald Schwank

    Article

    Savic et al Elife. 2018 May 29;7. pii: 33761. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    HA-2xNLS-SNAP-NLS and His6-MBP-TEV

    Expression

    Bacterial

    Mutation

    D10A, N580A

    Promoter

    Available since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mKate-pBAD

    Plasmid

    #54826

    Purpose

    Localization: Protein Expression Vector , Excitation: 588, Emission: 635

    Depositor

    Dmitriy Chudakov, Michael Davidson

    Article

    Shcherbo et al Nat Methods. 2007 Sep . 4(9):741-6.

    Type

    Empty backbone

    Use

    Tags

    6xHis and mKate

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pF710-pETDuet-1-Hs-delta19-ETFalpha-noHis wt

    Plasmid

    #85111

    Purpose

    expression of recombinant human mature (delta19) ETFalpha wt without tag

    Depositor

    Pal Falnes

    Article

    Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

    Insert

    human mature (delta19) ETFalpha (ETFA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRSET-B YFP

    Plasmid

    #108856

    Purpose

    Encodes for the fluorescent protein enhanced YFP (eYFP)

    Depositor

    Kalina Hristova

    Article

    Sarabipour et al Anal Biochem. 2014 Mar 15;449:155-7. doi

    Insert

    eYFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. BB3nK_ext_AD

    Plasmid

    #108687

    Purpose

    Plasmid for assembly of donor DNA templates consisting of three expression units

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET16b-Trx CC32/35SS

    Plasmid

    #36946

    Depositor

    Anna Blom

    Article

    King et al J Immunol. 2012 Apr 15;188(8):4103-12. E

    Insert

    Thioredoxin 1 (TXN Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    both active site cysteines (32,35) mutated to ser…

    Promoter

    T7

    Available since

    May 20, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pDuex2-iBB-GECO1

    Plasmid

    #194216

    Purpose

    Far-red fluorescent Ca2+ ion indicator for mammalian cell and bacterial expression.

    Depositor

    Robert Campbell

    Article

    Hashizume et al Protein Sci. 2022 Oct;31(10):e4440. doi:

    Insert

    iBB-GECO1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX Syt1 C2AB (96-421) D303G

    Plasmid

    #170646

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D303G

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX Syt1 C2AB (96-421) D365E

    Plasmid

    #170649

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D365E

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX Syt1 C2AB (96-421) I367T

    Plasmid

    #170652

    Purpose

    Expression of syt1 for purification in E. Coli

    Depositor

    Edwin Chapman

    Article

    Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

    Insert

    SYT1 (Syt1 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    I367T

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge