30,808 results

1. pJBL7005

   Plasmid

   #136944

   Purpose

   Malachite green aptamer with stability hairpin, T7 promoter

   Depositor

   Julius Lucks

   Article

   Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

   Insert

   Malachite green aptamer
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRE-Himar-131

   Plasmid

   #185923

   Purpose

   Himar transposon delivery plasmid that delivers a cyan fluorescent mTurquoise2 protein gene

   Depositor

   Mitja Remus-Emsermann

   Article

   Stocks et al Bacteria 2022, 1(1), 56-65

   Insert

   mTurquoise2
   

   Expression

   Bacterial

   Available Since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIA1385

   Plasmid

   #166862

   Purpose

   Codon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.

   Depositor

   Irina Artsimovitch

   Article

   Wang et al mBio. 2021 Jun 29;12(3):e0142321. doi: 1

   Insert

   SARS-COV-2 Nsp12 (ORF1ab Synthetic)
   

   Tags

   His-SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEK22

   Plasmid

   #210588

   Purpose

   Reporter gene expression in B. dendrobatidis

   Depositor

   Lillian Fritz-Laylin

   Article

   Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

   Insert

   BdH3pro-Hshph-G4S-nanoluc-ScADH1ter
   

   Use

   Luciferase

   Tags

   NanoLuc luciferase

   Expression

   Bacterial and Yeast

   Promoter

   BdH3pro

   Available Since

   Jan. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHN872

   Plasmid

   #178830

   Purpose

   Bacterial expression for human Saposin A

   Depositor

   Kazuhiro Abe

   Insert

   artificial gene encoding human Saposin A
   

   Tags

   GST, His8, TEV

   Expression

   Bacterial

   Mutation

   Codon-usage was optimized for bacterial expressio…

   Promoter

   T7

   Available Since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR11 CA NFAT2

   Plasmid

   #11793

   Depositor

   Anjana Rao

   Insert

   NFAT2 (Nfatc1 Mouse)
   

   Expression

   Bacterial

   Mutation

   Constitutively active: multiple serine to alanine…

   Available Since

   Aug. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

7. GLI K12

   Plasmid

   #16419

   Depositor

   Bert Vogelstein

   Article

   Kinzler et al Nature. 1988 Mar 24. 332(6162):371-4.

   Insert

   GLI (GLI1 Human)
   

   Expression

   Bacterial

   Available Since

   March 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET51b(+)-SNAP-CLIP

   Plasmid

   #127180

   Purpose

   Bacterial expression of the fused self-labeling tags SNAP-tag and CLIP-tag

   Depositor

   Kai Johnsson

   Article

   Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

   Insert

   SNAP-CLIP
   

   Tags

   HisTag and StrepTag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pdCas9+sgRNA expression vector precursor

   Plasmid

   #171798

   Purpose

   PICASSO: Library expression vector precursor for paired dCas9+sgRNA expression

   Depositor

   Stephen Elledge

   Article

   Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   pLtetO

   Available Since

   Aug. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pbacPuroR

    Plasmid

    #26348

    Depositor

    Ben Lehner

    Article

    Semple et al Nat Methods. 2012 Jan 30;9(2):118-9. doi

    Insert

    Bacterial promoter::PuroR::bacterial 3p flank
    

    Expression

    Bacterial

    Available Since

    Dec. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pProEx M-Ras(G22C) CL

    Plasmid

    #224268

    Purpose

    Protein purification of M-Ras (G22C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    M-Ras (MRAS Human)
    

    Expression

    Bacterial

    Mutation

    G22C

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pProEx Rit1(G47C) CL

    Plasmid

    #224267

    Purpose

    Protein purification of Rit1 (G47C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rit1 (RIT1 Human)
    

    Expression

    Bacterial

    Mutation

    G47C

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pProEx Ypt1(S17C) CL

    Plasmid

    #224265

    Purpose

    Protein purification of Ypt1 (S17C)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Ypt1 (YPT1 Budding Yeast)
    

    Expression

    Bacterial

    Mutation

    S17C

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pProEx Rap1A(G12C, L96F) CL

    Plasmid

    #224264

    Purpose

    Protein purification of Rap1A (G12C, L96F)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rap1A (RAP1A Human)
    

    Expression

    Bacterial

    Mutation

    G12C, L96F

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AWP-031

    Plasmid

    #213967

    Purpose

    Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for constitutive CRISPR array expression.

    Depositor

    Alexander Westermann

    Article

    Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121

    Insert

    Cas12a (cas12a Synthetic, Segatella bryantii)
    

    Tags

    3XFLAG

    Expression

    Bacterial

    Mutation

    D875A to inactivate target nuclease activity

    Promoter

    PcfxA, IPTG inducible

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBSKrnaSeph

    Plasmid

    #67506

    Purpose

    Express the tRNALys scaffold with the sephadex aptamer tag

    Depositor

    Luc Ponchon

    Article

    Ponchon et al Nat Protoc. 2009;4(6):947-59. doi: 10.10

    Insert

    modified tRNALys with sephadex aptamer
    

    Expression

    Bacterial

    Promoter

    lpp

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pJUMP24-1A(sfGFP)

    Plasmid

    #126971

    Purpose

    Level 1 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXTK-BB1 [Backbone_RK2-KanR_PartTypeBB]

    Plasmid

    #229387

    Purpose

    XanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_RK2-KanR_PartTypeBB and an sfGFP dropout

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pConRef-2H12

    Plasmid

    #221182

    Purpose

    cdGreen expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11213)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Inserts

    cdGreen
    mScarlet-I
    

    Expression

    Bacterial

    Mutation

    N-terminus changed: starts with MSKKYGEAVIKE ...

    Promoter

    Constitutive and None (same as cdGreen)

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. SUV420H2

    Plasmid

    #32864

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SUV420H2 (KMT5C Human)
    

    Tags

    His tag (with TEV protease)

    Expression

    Bacterial

    Available Since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits