32,934 results
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pB1H2 UV5 Zif268-5ala (2-hybrid bait plasmid)
Plasmid#128165PurposeUV5 promoter drives expression of Zif268-5alanine peptide fusion. Pair with pGHUC w-ERBIN as a negative control (doesn't turn on HIS3/GFP).DepositorInsert10 amino acid glycine/serine linker + 5alanines
UseSynthetic BiologyTagsFLAG and zif268ExpressionBacterialPromoterUV5Available SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mRFP1-kanMX6
Plasmid#133551PurposeYeast fluorescent (RFP) vector with PTEF promoter.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCA24N_6xHis-TEV-rpsA
Plasmid#83585PurposeExpress E. coli ribosomal protein S1 with a TEV-cleavable, N-terminal His tagDepositorAvailable SinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJYScr
Plasmid#132719PurposePlasmid for an easy-to-construct crRNA delivery vector using hybridized oligonucleotides. Constitutive transcription of crRNA, Spectinomycin resistanceDepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
KRigor980
Plasmid#129763PurposeExpresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfacesDepositorAvailable SinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBBR5pemIKpBAD
Plasmid#129273PurposeBroad host-range plasmid with toxin-antitoxin system for maintenance in the absence of antibiotic selection, and arabinose-inducible promoterDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGPΩTp
Plasmid#130660PurposeInsertional mutagenesis of GP transposon into chromosome of B. cenocepacia. Trimethoprim selection for plasmid.DepositorInsertΩ trimethoprim cassette
ExpressionBacterialMutationAddition of Ω sites next to trimethoprim cassette…Promoterdhfr promoterAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB3-mCherry-6xHis
Plasmid#130985PurposeBacterial expression of mouse EB3-mCherry-6xHis for protein purification.DepositorAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b-mEB3-eGFP-6xHis
Plasmid#130983PurposeBacterial expression of mouse EB3-GFP-6xHis for protein purification.DepositorAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pH6-TEV-mCherry
Plasmid#132430PurposeExpresses His6-tagged mCherry in E. coli using T7 promoterDepositorInsertmCherry
TagsN-terminal His6 tag with TEV protease siteExpressionBacterialMutationCodon optimizedPromoterT7Available SinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
FEV_pet28a
Plasmid#131668PurposeBacterial expression of ETS gene FEVDepositorAvailable SinceOct. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
ETV5_pet28a
Plasmid#131639PurposeBacterial expression of ETS gene ETV5DepositorAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ub-GyrA
Plasmid#128737PurposeExpresses Ub fused to GyrA intein at the C-terminalDepositorInsertUBC
TagsMxe GyrAExpressionBacterialPromoterT7Available SinceSept. 18, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAD16_CAD_12-490_pvp008
Plasmid#124842PurposeExpresses Aspergillus terreus CAD in E.coliDepositorInsertcis-aconitate decarboxylase
TagsStrepTag and TEV siteExpressionBacterialMutationdeleted amino acids 1-11, synthetic codon optimiz…PromoterT7Available SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
K401-Micro
Plasmid#122485PurposeComponent of optically-induced motor dimerization systemDepositorInsertHis tag MBP Kinesin 1-401 SspB micro
TagsH6-MBP-TEV and SspB microExpressionBacterialMutationKinesin amino acids 1-401Available SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
K401-iLid
Plasmid#122484PurposeComponent of optically-induced motor dimerization systemDepositorInsertKinesin 1-401 iLid His tag
TagsHis6 and iLidExpressionBacterialMutationKinesin amino acids 1-401PromoterT7Available SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEcRNAP6
Plasmid#128940PurposepET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, ZDepositorInsertsTagsHisExpressionBacterialMutationPreScission protease site (GE Healthcare; LEVLFQG…PromoterT7Available SinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_SNAP-p24
Plasmid#130718PurposePlasmid encoding the bacterial expression of a SNAP-fusion of p24 HIV Capsid proteinDepositorInsertSNAP-Tag-p24
TagsHisx10 and StrepTagExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…Available SinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCPP5912
Plasmid#128703PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcE-avrE1
ExpressionBacterialMutationThree synonymous mutations Ala 362 (GCT-GCC), Ala…Available SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only
