32,417 results
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Plasmid#198210PurposepJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing an optimal DQNAT sequon followed by a 6x-His tagDepositorInsertsfGFP_DQNAT
Tags6x His Tag and DQNAT glycosylation tagExpressionBacterialPromoterT7Available SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
NFAT5 luciferase
Plasmid#14110DepositorAvailable SinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMCSG53_N
Plasmid#167255PurposeBacterial expression of Nucleocapsid protein (residues 1-419)DepositorInsertN protein (residues 1-419) (N SARS-CoV-2)
TagsHis6-TEV siteExpressionBacterialMutationnoneAvailable SinceApril 27, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET-15b PAI-RBP
Plasmid#45390DepositorAvailable SinceJune 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNIC-ZB-SPRTNwt-FL
Plasmid#110217PurposeExpression in E. coli of full-length wild-type SPRTN protein, with His and ZB tags at N-terminus.DepositorAvailable SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPS0382
Plasmid#133232PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with constitutive promoter PsNifH (pOGG043), mCherry (EC15071) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBRα-σ70 D581G
Plasmid#53732PurposePlpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.DepositorInsertRNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
ExpressionBacterialMutationThe sigma 70 moiety carries the D581G substitutionPromoterlpp/lacUV5 (tandem promoter)Available SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAD12
Plasmid#34832DepositorInsertNone
UseRNAiExpressionBacterialAvailable SinceJune 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTmpSm
Plasmid#176228PurposeA template plasmid for amplification of the cI-hok-strA cassetteDepositorInsertcI of phage lambda, hok of the R1 plasmid, strA
UseSynthetic BiologyExpressionBacterialAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETDuet1_CoiC_CoiSA
Plasmid#208762PurposeTwo coi synthase CoiC and CoiSA, for the co-expression of CoiA1 in E.coli.DepositorInsertsCoiC
CoiSA
ExpressionBacterialMutationsingle nucleotide insertion (C70) at RBS of CoiCPromoterT7Available SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUG-natNT2
Plasmid#110922Purposeexpression of nat gene conferring resistance to nourseothricin for gene deletion in yeastDepositorInsertnat
ExpressionBacterial and YeastPromoterAgTEF1Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-IMX-DogTag
Plasmid#113765PurposeExpresses IMX-DogTag in bacterial cells for modular antigen multimerization using SnoopLigaseDepositorInsertIMX-DogTag
ExpressionBacterialPromoterT7Available SinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0075 TtCsm6 His6-TwinStrep-SUMO-BsaI
Plasmid#115270PurposeExpresses TtCsm6 from a SUMO-tagged bacterial expression constructDepositorInsertTtCsm6
TagsSUMOExpressionBacterialAvailable SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPGC-C
Plasmid#174580PurposepOPIN-GG expression vector compatible with C-terminal tagging, carbenicillin resistantDepositorTypeEmpty backboneExpressionBacterialAvailable SinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMH610
Plasmid#131862PurposeProduces holo-PhyB* in E. coliDepositorInsertsPhyB(1-651)-RGD-His6-Cys
HO1
PcyA
ExpressionBacterialPromoterT7 promoter-1 and T7 promoter-2Available SinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-pFF-41.5-mCherry
Plasmid#192863PurposepMUT2 based vector for sensing oxygen in E. coli Nissle 1917DepositorInsertOxygen inducible promoter
ExpressionBacterialAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX Su(fu) (CT#99)
Plasmid#13856DepositorInsertSu(fu) (SUFU Chicken)
TagsGSTExpressionBacterialMutationNco I fragment from full length; ~2kbp- ATG to st…Available SinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RT007-GCN4scFV-VB
Plasmid#163387Purpose2nd generation lentiviral vector to express anti-GCN4 module in the G-baToN systemDepositorInsertantiGCN4scFV-VEEGFRtm-BFP (GCN4scFV-VB)
UseLentiviralTagsBFPExpressionBacterial and MammalianPromoterEF1aAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
His6-PRC1
Plasmid#69111PurposeFor bacterial expression of recombinant human PRC1 tagged with His.DepositorAvailable SinceSept. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-KG-WASP-GBD
Plasmid#30113DepositorTagsGSTExpressionBacterialMutationContains the Cdc42-interacting domain of WASP (aa…Available SinceSept. 15, 2011AvailabilityAcademic Institutions and Nonprofits only