30,808 results

1. pET11_Ec_ORF209

   Plasmid

   #69029

   Purpose

   Expresses E.coli ORF209/ADPRase in bacterial cells

   Depositor

   Maurice Bessman, Sandra Gabelli

   Article

   Gabelli et al Nat Struct Biol. 2001 May;8(5):467-72.

   Insert

   ORF209/ADPRase
   

   Tags

   -

   Expression

   Bacterial

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

2. Wild-type CoV-2 ORF8

   Plasmid

   #159933

   Purpose

   Protein overexpression in E.coli

   Depositor

   James Hurley

   Article

   Flower et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

   Insert

   CoV-2 ORF8 18-121 (ORF8 CoV-2)
   

   Tags

   6x Histidine, TEV cleavable

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-A3A(N57Q)-BE3

   Plasmid

   #158157

   Purpose

   T7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLS

   Depositor

   Keith Joung

   Article

   Zeng et al Nat Med. 2020 Apr;26(4):535-541. doi: 10

   Insert

   6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
   

   Tags

   6xHis tag on N-terminus

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLBH533_MBP-Cas14b2 expression

   Plasmid

   #112506

   Purpose

   Expresses 10xHis-MBP fused to Cas14b2 under t7 promoter

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14b2
   

   Use

   CRISPR

   Tags

   MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYDE007

   Plasmid

   #194152

   Purpose

   sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, CarbR. Contains mrfp nontargeting control guide sequence.

   Depositor

   Edward Geisinger

   Article

   Bai et al J Bacteriol. 2021 May 20;203(12):e005652

   Insert

   sgRNA with mrfp control guide sequence
   

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJBL7004

   Plasmid

   #136943

   Purpose

   Malachite green aptamer, T7 promoter

   Depositor

   Julius Lucks

   Article

   Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

   Insert

   malachite green aptamer (MGA)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pT7

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. p007ampGFP

   Plasmid

   #58535

   Purpose

   GFP from jellyfish Aquorea sp. under T5 promoter with lac operators; ampicillin resistant.

   Depositor

   Jaroslaw Bryk

   Article

   Siddall et al bioRxiv 2021.06.20.449138

   Insert

   Green fluorescent protein
   

   Expression

   Bacterial

   Promoter

   T5 between two lac operators

   Available Since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. PGR-Blue

   Plasmid

   #68374

   Purpose

   Terminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.

   Depositor

   Nathan Reyna

   Type

   Empty backbone

   Use

   Synthetic Biology; Golden gate assembly

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. GSK3beta/pET29b

   Plasmid

   #16315

   Depositor

   Peter Klein

   Article

   Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

   Insert

   GSK3beta (gsk3b.L Frog)
   

   Expression

   Bacterial

   Available Since

   Dec. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

10. CBP-HAT-GST

    Plasmid

    #21093

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

    Insert

    CBP (Crebbp Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    HAT domain: aa 1196-1718

    Available Since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGex-4T-2_LC3A

    Plasmid

    #73946

    Purpose

    For recombinant expression of human LC3A/MAP1LC3A in E. coli

    Depositor

    Dieter Willbold

    Article

    Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

    Insert

    LC3A (MAP1LC3A Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pYTK081

    Plasmid

    #65188

    Purpose

    Encodes CEN6/ARS4 as a Type 7 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    CEN6/ARS4
    

    Expression

    Bacterial

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTU422

    Plasmid

    #178982

    Purpose

    13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization) enriched with CRISPR array spacer 1 (five times)

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Inserts

    gRAMP
    CRISPR array
    

    Tags

    Strep-Tag II SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGST-Vps4

    Plasmid

    #21495

    Depositor

    James Hurley

    Article

    Wollert et al Nature. 2009 Mar 12. 458(7235):172-7.

    Insert

    VPS4 (VPS4 Budding Yeast)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    none

    Available Since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTEV5_ RpMatB_1

    Plasmid

    #78205

    Purpose

    Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor

    Depositor

    Martin Webb

    Article

    Vancraenenbroeck et al ACS Chem Biol. 2015 Nov 20;10(11):2650-7

    Insert

    matB (His6 C106A R286C Q457C K488A)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    C106A R286C Q457C K488A

    Promoter

    T7

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pET His6 Sumo TEV expression vector with BioBrick polypromoter restriction sites (14-S)

    Plasmid

    #48313

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6 and Sumo

    Expression

    Bacterial

    Available Since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSLS.436

    Plasmid

    #184953

    Purpose

    Express -Eco1 RT and ncRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: ncRNA(wt) and RT
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHBJT001

    Plasmid

    #225152

    Purpose

    High copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.

    Depositor

    Jenny-Lee Thomassin

    Article

    Braun et al J Biosci Bioeng. 2024 Sep 6:S1389-1723(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    FLAG epitope tag (35 bp)

    Expression

    Bacterial

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-51b(+)-Caprola_13

    Plasmid

    #194674

    Purpose

    Protein production of the split HaloTag based calcium recorder Caprola_13 in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_13
    

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDL17_Cas9-His Δcys M1C

    Plasmid

    #206251

    Purpose

    Bacterial expression of SpCas9 Δcys M1C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    M1C, C80S, C574S

    Promoter

    T7

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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