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Plasmid#200967Purposeinduction of phase-separated condensate mediated by FUSN-IDRDepositorInsertFUSN
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pK1E-RNAP
Plasmid#48144PurposeShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylADepositorInsertrna polymerase K1E
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTriExEKAR2G2
Plasmid#40175DepositorInsertErk activity reporter
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDB007(+)
Plasmid#99147PurposeAccessory plasmid for PACE of MjTyrRS variantsDepositorInsertPT7 [SD8] gIII, luxAB; PproK tyrT(Opt, CUA)
UseTagsExpressionBacterialMutationPromoterPT7 and PproKAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDSP33
Plasmid#199375PurposeEncodes dual cistronic nuclear localized jGCaMP7f and mScarlet-I optimized for C. elegans expressionDepositorInsertNLS::jGCaMP7f::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
UseTagsExpressionBacterialMutationno mutations, except added subcellular localizati…PromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJBL725
Plasmid#140395PurposeExpresses SmtB protein under the control of T7-lacO promoterDepositorInsertT7-lacO-SmtB-T
UseTagsTEV-6XHisExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-mKelly1
Plasmid#119782PurposeExpresses mKelly1 tagged N-terminally with an MHHHHHHGSG tagDepositorInsertmKelly1
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET Biotin His6 mCitrine LIC cloning vector (H6-mCitrine)
Plasmid#29724DepositorTypeEmpty backboneUseTags6xHis, AviTag (biotin), TEV cleavage site, and mC…ExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSKDuet26
Plasmid#41689DepositorInsertTvo VMA intein
UseTagsGB1 and His-tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
CBX7
Plasmid#25241PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCBX7_04 (CBX7 Human)
UseTags6x His and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX6P1-DEST-FLAG
Plasmid#119754PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal FLAG tag.DepositorTypeEmpty backboneUseTagsFLAG and GSTExpressionBacterialMutationPromotertac promoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHS0766 pET45b(+) His14-MBP-TEV-AloTnpB-2 3' extension
Plasmid#176542PurposeBacterial expression of His14-MBP-TEV-AloTnpB-2 with 3' extension derived from locusDepositorInsertsMBP tag
TnpB
UseTags3' extension derived from endogenous locus a…ExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21a-TEX264_wt-His6
Plasmid#220214PurposeBacterial expression of wild type TEX264, C-terminal His6 tagDepositorInsertTEX264 (TEX264 Human)
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pet15_rbsk
Plasmid#220227PurposeBacterial expression of Rbsk in E. coli BL21_DE3 (pLys) or similarDepositorInsertribokinase (rbsK E.coli)
UseTagsHistagExpressionBacterialMutationPromoterT7Available sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET3C-ARL16(CO)-His6
Plasmid#216485PurposeUsed for expression of human ARL16, short form, in bacteria. The open reading frame has been codon optimized for bacterial expression and contains a glycine rich linker and His6 tag at the C-terminus.DepositorInsertADP-ribosylation factor-like 16 (ARL16 Human)
UseTagsincludes GSGSGSHHHHHH (His6) tag at C-terminusExpressionBacterialMutationPromoterT7Available sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-CV-Ykt6
Plasmid#168030PurposeExpresses eCFP-Ykt6(Q131-K190)-mVenus in bacterial cellsDepositorInserteCFP-Ykt6(Q131-K190)-mVenus
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDSG321
Plasmid#115598Purposesurface-displayed Nb3, used in composability experiments; medium copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK1037
Plasmid#8833DepositorInsertTVMV protease, catalytic domain
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
KG#121
Plasmid#110879PurposeExpresses the C. elegans unc-31 cDNA pan-neuronallyDepositorInsertsrab-3 promoter
unc-31 cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationMissing first 408bpPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -