30,327 results

1. pBAD-D4

   Plasmid

   #37473

   Depositor

   Roger Tsien, Amy Palmer

   Article

   Palmer et al Chem Biol. 2006 May;13(5):521-30.

   Insert

   D4 second generation cameleon (calcium sensor)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ara

   Available since

   Oct. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pAB078d8

   Plasmid

   #79206

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PlacZ-opt (OR1+2) luxAB; Ppro1 434cI-SH2ABL1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PlacZ-opt (OR1+2); Ppro1

   Available since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSL0762 (pQCascade_crRNA-nt)

   Plasmid

   #130636

   Purpose

   Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a non-targeting guide RNA.

   Depositor

   Samuel H. Sternberg

   Article

   Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(non-targeting)
   

   Use

   CRISPR; Transposon

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKSJ376

   Plasmid

   #103065

   Purpose

   Expresses colicin E1 with R domain deletion and ImmE1

   Depositor

   Alan Finkelstein, Karen Jakes

   Article

   Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0

   Inserts

   colicin E1 immunity protein (pColE1_26, imm E. coli)
   cea/colicin E1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   R (receptor-binding domain) deletion in colicin E…

   Promoter

   T7 lac (from pET) and T7 lac (from pET52-b)

   Available since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET mCerulean LIC cloning vector (u-mCerulean)

   Plasmid

   #29773

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTXB1 EGFP

   Plasmid

   #129442

   Purpose

   expression of intein/ gfp

   Depositor

   Oskar Laur

   Insert

   intein/ gfp
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBFC0625

   Plasmid

   #186691

   Purpose

   VcDART under control of Lac promoter, Vc_lacZ_α_1 gRNA, GmR+sfGFP cargo

   Depositor

   Jennifer Doudna

   Article

   Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

   Inserts

   tnsA
   tnsB
   tnsC
   tnsD
   tniQ
   cas8-cas5 fusion
   cas7
   cas6
   Vc_lacZ_α_1 Spacer crRNA
   Tn7 transposon
   GmR+sfGFP VcDART Cargo
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLac

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. p4028 GST-E6AP

   Plasmid

   #8655

   Depositor

   Peter Howley

   Article

   Oda et al Proc Natl Acad Sci U S A 1999 Aug 17;96(

   Insert

   E6-AP (UBE3A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 26, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST-GST-FBW7 alpha

   Plasmid

   #197456

   Purpose

   The plasmid expresses GST-tagged FBW7 human isoform 1. Used for in vitro translation of FBW7alpha.

   Depositor

   Omar Khan

   Article

   Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

   Insert

   FBW7 (FBXW7 Human)
   

   Use

   In vitro translation

   Tags

   GST

   Expression

   Bacterial

   Mutation

   See depositor comments below

   Promoter

   T7

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET29a-hNABP2

    Plasmid

    #128307

    Purpose

    Express hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tag

    Depositor

    Yuliang Wu

    Article

    Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

    Insert

    Human Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Y85C- please see depositor comments below

    Promoter

    T7

    Available since

    Aug. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDsRed-monomer-hAIM2

    Plasmid

    #51544

    Purpose

    Express DsRed monomer-tagged hAIM2 in bacteria

    Depositor

    Emad Alnemri

    Article

    Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

    Insert

    AIM2 (AIM2 Human)
    

    Use

    Tags

    DsRed monomer

    Expression

    Bacterial

    Mutation

    Promoter

    lac operator

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSBY3_ligD

    Plasmid

    #104624

    Purpose

    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD

    Depositor

    Sheng Yang

    Article

    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    FnCpf1 codon-optimized C.glutamicum

    Promoter

    hsp60

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMS12: pDonor(AvCAST)

    Plasmid

    #168145

    Purpose

    AvCAST donor harboring non-functional cargo.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    AvCAST right end (RE)
    AvCAST left end (LE)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMMBGent

    Plasmid

    #45475

    Depositor

    Michele Swanson

    Article

    Hammer et al Mol Microbiol. 1999 Aug;33(4):721-31.

    Insert

    Gentamycin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUC-UAS1B8-TEF(272)

    Plasmid

    #44376

    Depositor

    Hal Alper

    Article

    Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

    Insert

    UAS1B8-TEF(272) promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    UAS1B8-TEF(272)

    Available since

    April 16, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pET303-colE1TR

    Plasmid

    #155181

    Purpose

    Expresses colicin E1 residues 1-364

    Depositor

    Joanna Slusky

    Article

    Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

    Insert

    colicin E1 residues 1-364
    

    Use

    Tags

    C-terminal 6x Histidine tag

    Expression

    Bacterial

    Mutation

    truncation of colicin E1 including 1-364

    Promoter

    T7

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX6P-1-SF2

    Plasmid

    #99020

    Purpose

    Bacterial expression of SF2

    Depositor

    Honglin Chen

    Article

    Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

    Insert

    SF2 (SRSF1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. MP28444 – pFUSE2ss-aRD114_CHIg-mIgG2A

    Plasmid

    #110555

    Purpose

    pFUSE backbone with an IL2 signal sequence, expressing the variable heavy chain of anti-RD114 binder fused to murine IgG2A constant heavy chain.

    Depositor

    Martin Pule

    Article

    Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg

    Insert

    Anti-RD114 heavy chain
    

    Use

    Tags

    mIgG2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF-1 alpha core promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZac2.1 gfaABC1D-Glt1-BioID2-BioID2-HA

    Plasmid

    #176745

    Purpose

    Expresses Glt-1 fused with BioID2 at the C-terminus in astrocytes

    Depositor

    Baljit Khakh

    Article

    Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

    Insert

    Glt1-BioID2-BioID2-HA
    

    Use

    AAV

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    gfaABC1D

    Available since

    April 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSN007

    Plasmid

    #102440

    Purpose

    For PCR amplification to create paired gRNAs to clone into Golden Gate gRNA expression vectors (e.g. pSB700 Plasmid #64046)

    Depositor

    Alejandro Chavez, George Church

    Article

    Nageshwaran et al J Vis Exp. 2018 Oct 2;(140). doi: 10.379

    Insert

    7SK paired gRNA insert
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge