32,218 results

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pET48 AtGUN1-PPR1

Plasmid

#136359

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 708)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 708 (GUN1 Mustard Weed)

Tags

TRX-His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET48 AtGUN1-PPR2

Plasmid

#136360

Purpose

Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 619)

Depositor

Tatsuru Masuda

Article

Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

Insert

cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 619 (GUN1 Mustard Weed)

Tags

TRX-His

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMS18: pHelper(PmcCAST)_PmcPSP1

Plasmid

#168151

Purpose

Inducible expression of PmcCAST proteins with crRNA targeting PmcPSP1.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
PmcTniQ

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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AlphaB

Plasmid

#124524

Purpose

pColE1_AlphaB

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial, Insect, and Mamm…

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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AlphaD

Plasmid

#124526

Purpose

pColE1_AlphaD

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial, Insect, and Mamm…

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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GST-FBL

Plasmid

#179245

Purpose

protein purification in E. coli

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

FBL (FBL Human)

Tags

GST

Expression

Bacterial

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSM015

Plasmid

#128289

Purpose

asymmetric plasmid partitioning E. coli - regulatory plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Para - lp-cfp-parB

Expression

Bacterial

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pXN-FBLWLF-LexA

Plasmid

#26266

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

LexA

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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C331

Plasmid

#141123

Purpose

RhlI expressing plasmid

Depositor

Matthew Bennett

Article

Alnahhas et al Nat Commun. 2020 Jul 21;11(1):3659. doi:

Insert

RhlI (rhlI )

Tags

ssrA degradation tag

Expression

Bacterial

Promoter

engineered lac promoter

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-His-TEV-OTC_Ub

Plasmid

#172134

Purpose

Scaﬀold design for studying ubiquitin interacting proteins by cryo-EM.

Depositor

Shang-Te Danny Hsu

Article

Chiu et al Biochemistry. 2021 Apr 13;60(14):1075-10

Insert

E. coli ornithine transcarbamylase (OTC), fused to human ubiquitin

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET-FLAG-SpCas9-HF1

Plasmid

#126770

Purpose

Expression of increased fidelity SpCas9-HF1 in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

SpCas9-HF1

Use

CRISPR

Tags

3xFLAG, 6xHis, MBP, and NLS

Expression

Bacterial

Mutation

N497A, R661A, Q695A, Q926A

Promoter

T7

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 p37

Plasmid

#113500

Purpose

Bacterial expression of GST-tagged p37

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p37 (Ubxn2b Mouse)

Tags

GST-PreScission protease site

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pETM6-C4-mCherry

Plasmid

#66530

Purpose

Expresses mCherry under the control of Mutant T7 Promoter C4

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon Optimized for E. coli

Promoter

Mutant T7 Promoter - C4

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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pAB061i2

Plasmid

#79229

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

Ppro1 rpoZ-HA4 (Y87A); PlacIq lacI

Expression

Bacterial

Promoter

Ppro1; PlacIq

Available Since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pOGG216

Plasmid

#114002

Purpose

Level 2 golden gate cloning vector for medium copy number free-living expression

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Type

Empty backbone

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pBTL-3

Plasmid

#22807

Depositor

Ryan Gill

Article

Lynch et al Biotechnol Bioeng. 2006 May 5. 94(1):151

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

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Reb_65 aka PAS626

Plasmid

#71231

Purpose

Reb_1 with RebA(Y82A)

Depositor

Pamela Silver

Article

Polka et al ACS Synth Biol. 2016 Feb 3.

Insert

Reb locus

Tags

none

Expression

Bacterial

Mutation

RebA(G53A, Y82A)

Available Since

April 7, 2016

Availability

Academic Institutions and Nonprofits only
pFVTd5

Plasmid

#167520

Purpose

Broad-Host range expression vector, RSF1010 origin, VanRAM repressor, PVanCC promoter, tdKatushka2, Gentamicin Resistance Marker

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

VanRAM-tdKatushka2

Use

Synthetic Biology

Expression

Bacterial

Promoter

PVanCC

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pSV272-PfMBP-HypDAE

Plasmid

#110296

Purpose

Expresses t-4-hydroxy-L-proline dehydratase activating enzyme in E. coli BL21(DE3)

Depositor

Emily Balskus

Article

Levin et al Science. 2017 Feb 10;355(6325). pii: 355

Insert

t-4-Hydroxy-L-proline Dehydratase Activating Enzyme

Tags

His6 and Maltose Binding Protein

Expression

Bacterial

Promoter

T7

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pColdII-SIRPT1-Xenopus (Silurana) tropicalis

Plasmid

#75355

Purpose

Nt His-tag Xenopus (Silurana) tropicalis Sacsin 87-1376

Depositor

Kalle Gehring

Insert

Sacsin Xenopus (Silurana) tropicalis 87-1376

Tags

His-Tag

Expression

Bacterial

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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USP21 (3MTN)

Plasmid

#28112

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

USP21 (USP21 Human)

Tags

His tag with Thrombin cleavage

Expression

Bacterial

Mutation

catalytic domain only (amino acid residues 209-56…

Available Since

Feb. 28, 2011

Availability

Industry, Academic Institutions, and Nonprofits
pTXB1-H2B(1-114)_wt

Plasmid

#118252

Purpose

Bacterial expression plasmid encoding X. laevis H2B residues 1-114 as a chimeric fusion to GyrA intein with chitin binding domain at the C-terminus. H2B fragment can be derivatized using MESNa

Depositor

Cynthia Wolberger

Article

Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

Insert

xlH2B(1-114)

Tags

Mxe-GyrA intein fused to chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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DMPK

Plasmid

#38892

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

DMPK (DMPK Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pHH0103_PIKFYVE_DEP

Plasmid

#109789

Purpose

Protein expression and purification of PIKFYVE_DEP

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PIKFYVE_DEP (PIKFYVE Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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C163

Plasmid

#141127

Purpose

Expresses sfCFP under lac promoter

Depositor

Matthew Bennett

Article

Alnahhas et al Nat Commun. 2020 Jul 21;11(1):3659. doi:

Insert

sfCFP

Tags

ssrA degradation tag

Expression

Bacterial

Promoter

engineered lac promoter

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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C164

Plasmid

#141128

Purpose

Expresses sfYFP under lac promoter

Depositor

Matthew Bennett

Article

Alnahhas et al Nat Commun. 2020 Jul 21;11(1):3659. doi:

Insert

sfYFP

Tags

ssrA degradation tag

Expression

Bacterial

Promoter

engineered lac promoter

Available Since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

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rnALDH

Plasmid

#67920

Purpose

Expresses aldehyde dehydrogenase from Rattus norvegicus, codon optimized for expression in E. coli

Depositor

Justin Siegel

Insert

Rattus norvegicus aldehyde dehydrogenase (Aldh3a1 Rat)

Tags

6x-His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
foxn4

Plasmid

#11260

Depositor

Lisa Steiner

Article

Danilova et al Brain Res Dev Brain Res. 2004 Oct 15. 15

Insert

foxn4 (foxn4 Zebrafish)

Expression

Bacterial

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pUC-UAS1B8-TEF(504)

Plasmid

#44372

Depositor

Hal Alper

Article

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(504) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(504)

Available Since

July 15, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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SARS-CoV-2_N_deltaNIDR

Plasmid

#157868

Purpose

express MBP tagged SARS-CoV-2 Nucleocapsid protein without the N-terminal IDR

Depositor

Nicolas Fawzi

Article

Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

Insert

N (N SARS-CoV-2)

Tags

MBP

Expression

Bacterial

Mutation

deltaNIDR

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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