30,808 results

1. pBL066c

   Plasmid

   #227937

   Purpose

   Express Aβ42-GFP

   Depositor

   Tina Wang

   Article

   Yang et al Nat Chem Biol. 2025 Jan 13. doi: 10.1038

   Insert

   Aβ42
   

   Tags

   GFP (F64L, S65T)

   Expression

   Bacterial

   Mutation

   F64L and S65T in GFP

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSB_syb25

   Plasmid

   #197992

   Purpose

   Expressed sybody 25 as a C-terminal myc-His-tagged construct

   Depositor

   Simon Newstead

   Article

   Parker et al Nat Struct Mol Biol. 2023 Jul 23. doi: 1

   Insert

   Sybody 25
   

   Tags

   Myc-His

   Expression

   Bacterial

   Promoter

   Arabinose

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAP973-1

   Plasmid

   #99496

   Purpose

   TEV::linker::meGFP:3XFlag

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Methods. 2017 May 15;121-122:86-93. doi:

   Insert

   TEV::linker::meGFP::3XFlag
   

   Expression

   Bacterial

   Available Since

   Sept. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKM263-ScPPX

   Plasmid

   #38327

   Depositor

   Florian Freimoser

   Article

   Werner et al Arch Microbiol. 2005 Nov;184(2):129-36.

   Insert

   PPX1 (PPX1 Budding Yeast)
   

   Tags

   GST, His, and TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJJS011

   Plasmid

   #188334

   Purpose

   PCR template for repair templates to tag a gene with a mTagBFP2 and mIAA7 AID degron using homology-directed repair for C. elegans.

   Depositor

   Mike Boxem

   Article

   Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

   Insert

   mIAA7::mTagBFP2::Linker
   

   Expression

   Bacterial

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBBR5pemIKpBAD

   Plasmid

   #129273

   Purpose

   Broad host-range plasmid with toxin-antitoxin system for maintenance in the absence of antibiotic selection, and arabinose-inducible promoter

   Depositor

   Lindsey Burbank

   Article

   Burbank et al Microbiol Resour Announc. 2019 Sep 5;8(3

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKB083

   Plasmid

   #181952

   Purpose

   IPTG-inducible expression of PhoQ

   Depositor

   Jeffrey Tabor

   Article

   Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

   Insert

   PhoQ; lacI (lacI PhoQ - Salmonella enterica subsp. enterica serovar Typhimurium; lacI - E. coli)
   

   Expression

   Bacterial

   Promoter

   PhoQ-Ptac; lacI-PlacIq

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBFC0625

   Plasmid

   #186691

   Purpose

   VcDART under control of Lac promoter, Vc_lacZ_α_1 gRNA, GmR+sfGFP cargo

   Depositor

   Jennifer Doudna

   Article

   Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

   Inserts

   tnsA
   tnsB
   tnsC
   tnsD
   tniQ
   cas8-cas5 fusion
   cas7
   cas6
   Vc_lacZ_α_1 Spacer crRNA
   Tn7 transposon
   GmR+sfGFP VcDART Cargo
   

   Expression

   Bacterial

   Promoter

   PLac

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTAT2.2

   Plasmid

   #35621

   Depositor

   Steven Dowdy

   Article

   Wadia et al Nat Med. 2004 Mar;10(3):310-5. Epub 2004

   Type

   Empty backbone

   Tags

   6xHIS, T7 tag, and TAT

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 23, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pRSFDuet-1_VNp-Uricase

    Plasmid

    #182401

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-uricase fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-uricase
    

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pACT2-CAS9c

    Plasmid

    #51055

    Purpose

    Express Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.

    Depositor

    Yunde Zhao

    Article

    Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

    Insert

    Cas9c
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Bacterial and Yeast

    Promoter

    yeast ADH1 promoter

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-Nsp14-TEV-6His

    Plasmid

    #154952

    Purpose

    Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli

    Depositor

    Justin Kollman

    Insert

    Nsp14-TEV-6His (ORF1ab SARS-CoV-2)
    

    Tags

    TEV-6His

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pDESTsmg

    Plasmid

    #18878

    Depositor

    Ted Baker

    Article

    Goldstone et al Protein Expr Purif. 2008 Jan . 57(1):81-

    Type

    Empty backbone

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLIPrbs

    Plasmid

    #17860

    Depositor

    Wolf Frommer

    Article

    Lager et al FEBS Lett. 2003 Oct 9. 553(1-2):85-9.

    Insert

    FLIPrbs
    

    Tags

    ECFP and EYFP

    Expression

    Bacterial

    Mutation

    D208G

    Available Since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. R725-X11-566: His6-MBP-tev-Hs.LGALS3

    Plasmid

    #159699

    Purpose

    E. coli protein expression of His6-MBP-tev-Hs.LGALS3

    Depositor

    Dominic Esposito

    Article

    Lakshman et al J Biol Chem. 2019 Feb 8;294(6):2193-2207

    Insert

    His6-MBP-tev-Hs.LGALS3 (LGALS3 Human)
    

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Available Since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a(+) 6xHis-3xFlag-IFIT5

    Plasmid

    #53561

    Purpose

    bacterial expression of IFIT5

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT5 (IFIT5 Human)
    

    Tags

    3xFLAG, 6xHIS, and TEV site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pNP561

    Plasmid

    #222723

    Purpose

    Target and Recombinase Golden Gate Landing Pad Backbone for LT-RT with T7 Recombinase

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Type

    Empty backbone

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. poLuc1

    Plasmid

    #154128

    Purpose

    Expresses orthogonal firefly luciferase gene in bacterial cells

    Depositor

    Alexander Mankin

    Article

    Aleksashin et al Nat Commun. 2020 Apr 20;11(1):1858. doi:

    Insert

    orthogonal firefly luciferase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX6p-1-CYB5R3 deltaN26

    Plasmid

    #204557

    Purpose

    Expresses GST-tagged CYB5R3 deltaN26 in E.coli

    Depositor

    Masaaki Komatsu

    Article

    Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

    Insert

    CYB5R3 (CYB5R3 Human)
    

    Expression

    Bacterial

    Mutation

    delta1-26

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSBY3_ligD

    Plasmid

    #104624

    Purpose

    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD

    Depositor

    Sheng Yang

    Article

    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

    Insert

    FnCpf1
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    FnCpf1 codon-optimized C.glutamicum

    Promoter

    hsp60

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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