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pKL11-GCaMP6f bb100

Plasmid

#158983

Purpose

Constitutive expression (sigma 70 promoter) of GCaMP6f fluorophore in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

GCaMP6f

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#100833

Promoter

Bba_J23100 (sigma 70)

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Hchk2 (kin-)-PUNI10

Plasmid

#41908

Depositor

Stephen Elledge

Article

Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

Insert

CHK2 (CHEK2 Human)

Expression

Bacterial

Mutation

kinase dead (D347A)

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only
pYTRW22K_7Ti1

Plasmid

#177293

Purpose

genomic integration of genes (inserted in I-SceI-site) via rrn-interposon with TcR and LacZ

Depositor

Karl-Erich Jaeger

Article

Weihmann et al FEMS Microbes. 2022 Dec 19;4:xtac030. do

Insert

lacZ

Use

Synthetic Biology; Yeast expression, rrn genomic …

Expression

Bacterial

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pMulti_S_ccdB

Plasmid

#223853

Purpose

GEC-acceptor for 4G-cloning, designed for expression in E. coli (Streptomycin resistance)

Depositor

Stephan Gruber

Article

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Type

Empty backbone

Use

Golden-gate acceptor

Expression

Bacterial

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX2T-TEV CHMP1B 1-143

Plasmid

#108286

Purpose

Expresses residues 1-143 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-278

Depositor

Wesley Sundquist

Article

Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

Insert

CHMP1B (CHMP1B Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-143

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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SNX10-PX (1-201)

Plasmid

#119091

Purpose

Bacterial expression of human phox homology (PX) domain, SNX10-PX (1-201)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX10-PX (1-201) (SNX10 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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NUDT3A

Plasmid

#42348

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

NUDT3A (NUDT3 Human)

Tags

His6-TEV

Expression

Bacterial

Mutation

See Comments

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
p2attB-FST344-HA-CPL

Plasmid

#131460

Purpose

Encodes human Follistatin protein

Depositor

Michele Calos

Article

Guha et al Mol Ther Methods Clin Dev. 2019 Oct 14;1

Insert

Follistatin (FST Human)

Tags

HA

Expression

Bacterial and Mammalian

Available Since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNA500

Plasmid

#149640

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pET Biotin His6 mOrange LIC cloning vector (H6-mOrange)

Plasmid

#29723

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, AviTag (biotin), TEV cleavage site, and mO…

Expression

Bacterial

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pREDusk-StrR-MCS

Plasmid

#188972

Purpose

For red light-repressed bacterial expression of gene of interest (StrR)

Depositor

Heikki Takala

Article

Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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D. vulgaris Cascade/I-C (Cas5c-Cas8c-Cas7)/pHMGWA

Plasmid

#81185

Purpose

Expresses Desulfovibrio vulgaris Cascade/I-C complex subunits in E. coli with TEV-cleavable N-terminal His-MBP tag on Cas5c

Depositor

Jennifer Doudna

Article

Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

Inserts

Cas5c
Cas8c
Cas7

Use

CRISPR

Tags

6xHis - MBP - TEV protease site

Expression

Bacterial

Promoter

T7

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pYTK-DN5

Plasmid

#180286

Purpose

NatR multi-gene yeast expression vector (GFP-dropout)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConLS' - GFP - ConRE' - NatR - CEN6/ARS4

Expression

Bacterial and Yeast

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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BtARR3 short (1-392)

Plasmid

#236272

Purpose

Bacterial expression of bovine arrestin-3 short (1-392) L393Stop

Depositor

Vsevolod Gurevich

Article

Vishnivetskiy et al Curr Protoc. 2023 Sep;3(9):e832. doi: 10

Insert

arrestin-3 (ARR3 Bovine)

Expression

Bacterial

Mutation

short, (1-392) L393Stop

Promoter

lac

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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BtARR3 long

Plasmid

#236266

Purpose

Bacterial expression of bovine arrestin-3 long isoform

Depositor

Vsevolod Gurevich

Article

Vishnivetskiy et al Curr Protoc. 2023 Sep;3(9):e832. doi: 10

Insert

arrestin-3 (ARR3 Bovine)

Expression

Bacterial

Mutation

long isoform

Promoter

lac

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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BtARR2 Cys-less

Plasmid

#236273

Purpose

Bacterial expression of bovine arrestin-2 C59V, C125S, C140L, C150V, C242V, C251V, C269S

Depositor

Vsevolod Gurevich

Article

Zhuo et al J Biol Chem. 2014 Jul 25;289(30):20991-1

Insert

arrestin-2 (ARRB2 Bovine)

Expression

Bacterial

Mutation

C59V, C125S, C140L, C150V, C242V, C251V, C269S

Promoter

lac

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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BtARR2 long

Plasmid

#236268

Purpose

Bacterial expression of bovine arrestin-2 long isoform

Depositor

Vsevolod Gurevich

Article

Vishnivetskiy et al Curr Protoc. 2023 Sep;3(9):e832. doi: 10

Insert

arrestin-2 (ARRB2 Bovine)

Expression

Bacterial

Mutation

long isoform

Promoter

lac

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pET24TEV-tSHIP1

Plasmid

#183770

Purpose

Expresses C-terminal truncated SHIP1 (tSHIP1)with C-terminal TEV and 6HIS tags from pET24 backbone

Depositor

William Kerr

Article

Sudan et al Front Immunol. 2022 May 4;13:830961. doi

Insert

SHIP1 (INPP5D Human)

Tags

6HIS and Tobacco Etch Virus (TEV) Cleavage site

Expression

Bacterial

Mutation

includes aa1 to aa883 only

Promoter

T7 lac

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pEANHISECArdB

Plasmid

#96967

Purpose

Expression of E. coli ArdB protein wirh an N-terminal TEV protease cleavable His tag

Depositor

Huanting Liu, James Naismith

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

E. coli ArdB

Tags

N-ter TEV cleavable 6His

Expression

Bacterial

Mutation

none

Promoter

T7

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pVRa14_3200

Plasmid

#49666

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF14_3200

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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