30,810 results

1. pLC291

   Plasmid

   #44448

   Depositor

   Christopher Marx

   Article

   Chubiz et al BMC Res Notes. 2013 May 6;6:183. doi: 10

   Type

   Empty backbone

   Use

   Rk2, pmb1

   Expression

   Bacterial

   Promoter

   P_R (Phage 16-3)

   Available Since

   Aug. 30, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCas9-beta

   Plasmid

   #169240

   Purpose

   Cas9 and beta expression plasmid for performing gene knockouts via oligo recombineering

   Depositor

   Brian Pfleger

   Article

   Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

   Inserts

   cas9
   beta
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD-TDsmURFP-RBS-HO-1

   Plasmid

   #80342

   Purpose

   Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in bacterial cells.

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

   Inserts

   Tandem Dimer smURFP
   HO-1
   

   Tags

   6 Histidine Tag

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTC-28S15-0X

   Plasmid

   #20316

   Depositor

   Dirk Schnappinger

   Article

   Klotzsche et al Nucleic Acids Res. 2009 Apr;37(6):1778-8

   Insert

   Psmyc-tetR#28
   

   Expression

   Bacterial

   Available Since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tus pGST-//

   Plasmid

   #53305

   Purpose

   Expresses GST-tagged E.coli replication terminator protein Tus.

   Depositor

   John Lis

   Article

   Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

   Insert

   E.Coli Tus replication terminator protein
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-2T-Cdc42-Q61L

   Plasmid

   #12971

   Depositor

   Gary Bokoch

   Insert

   Cdc42 constitutively active (CDC42 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Q61L Constitutively active

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

7. GPX3

   Plasmid

   #38899

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   GPX3 (GPX3 Human)
   

   Tags

   Tev His Flag

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pET GFP domain 11 LIC cloning vector (GFP C-11)

   Plasmid

   #30183

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   6xHis, AviTag (biotin), GFP domain 11, and TEV cl…

   Expression

   Bacterial

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. EYFP-Talin1-FERM

   Plasmid

   #110565

   Purpose

   bacterial expresion of EYFP-tagged Talin 1

   Depositor

   Tina Izard

   Insert

   Talin 1 (Tln1 Mouse)
   

   Tags

   (His)6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-51b(+)-cpHalo∆

    Plasmid

    #194659

    Purpose

    Protein production of the split HaloTag part cpHalo∆ (cpHaloTag135/156) in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    cpHalo∆
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AURKA_HUMAN_D0

    Plasmid

    #79739

    Purpose

    This plasmid encodes the kinase domain of AURKA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    AURKA (AURKA Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

12. hnRNPA2_LC_WT

    Plasmid

    #98657

    Purpose

    Expresses 6xHis-tagged hnRNPA2 AA 190-341

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGM190

    Plasmid

    #69614

    Purpose

    inducible gene expression in Streptomyces

    Depositor

    Günther Muth

    Article

    Reuther et al Mol Microbiol. 2006 Jul;61(2):436-46. do

    Inserts

    aphII
    tsr
    rep
    sso
    TipA promoter
    dso
    

    Use

    Streptomyces- e. coli shuttle vector

    Expression

    Bacterial

    Available Since

    Jan. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET32-sLatherin

    Plasmid

    #61685

    Purpose

    bacterial expression of the horse sweat/saliva protein Latherin as a His-tagged thioredoxin fusion

    Depositor

    Brian Smith

    Article

    Vance et al J R Soc Interface. 2013 Jun 19;10(85):20

    Insert

    Latherin (LATH horse)
    

    Tags

    HIS and Trx-Tag thioredoxin protein

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    T7

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pET51b(+)_LAMA-F98 (for GFP)

    Plasmid

    #130714

    Purpose

    Plasmid encoding the bacterial expression of LAMA-F98 for GFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    LAMA-F98
    

    Tags

    Hisx10

    Expression

    Bacterial

    Mutation

    Deleted first Met, Ala and Last Ser from the Enha…

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCSW21

    Plasmid

    #86836

    Purpose

    bacterial expression of anti-CRISPR protein AcrIIA4

    Depositor

    Joseph Bondy-Denomy

    Article

    Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

    Insert

    acrIIA4
    

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. 137 pGEX2TK RBAP2 HA

    Plasmid

    #11174

    Depositor

    William Sellers

    Insert

    RBAP2 (ARID4A Human)
    

    Tags

    GST and HA

    Expression

    Bacterial

    Available Since

    Jan. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pNMTMA_MBP-Cav1-His6

    Plasmid

    #223700

    Purpose

    To produce standard caveospheres (without the Z-domain), Parton Lab clone LDB

    Depositor

    Rob Parton

    Article

    T. D. et al bioRxiv

    Insert

    MBP-Cav1-His6
    

    Expression

    Bacterial

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. V621 pGEX-4T-LP - Acceptor

    Plasmid

    #11715

    Depositor

    Tony Pawson

    Article

    Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

    Type

    Empty backbone

    Use

    Cre/Lox; Acceptor vector

    Tags

    GST

    Expression

    Bacterial

    Available Since

    May 24, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pCMW35 p21 (mouse)

    Plasmid

    #16478

    Depositor

    Bert Vogelstein

    Article

    el-Deiry et al Cancer Res. 1995 Jul 1. 55(13):2910-9.

    Insert

    p21 (Cdkn1a Mouse)
    

    Expression

    Bacterial

    Available Since

    April 7, 2008

    Availability

    Academic Institutions and Nonprofits only