30,327 results

1. pET30a-FluA-NP

   Plasmid

   #165092

   Purpose

   protein expression in E. coli

   Depositor

   Rick Tarleton

   Article

   Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

   Insert

   Influenza A virus(A/Georgia/03/2019(H1N1) nucleocapsid protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 11, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pET26_MraYaa

   Plasmid

   #100166

   Purpose

   recombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target protein

   Depositor

   Seok-Yong Lee

   Article

   Chung et al Science. 2013 Aug 30;341(6149):1012-1016

   Insert

   mraY (phospho-MurNAc-pentapeptide translocase)
   

   Use

   Tags

   MBP-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHG7

   Plasmid

   #188255

   Purpose

   Integrating plasmid contains the C31 int/attP locus and the hygromycin-resistance gene for selection

   Depositor

   Hong Gao, Maggie Smith

   Article

   Gao et al Access Microbiol. 2021 Dec 8;3(12):00029

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   act1p/actII-orf4

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJUMP44-2A(sfGFP)

   Plasmid

   #126988

   Purpose

   Level 2 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET48 AtGUN1-PS

   Plasmid

   #136358

   Purpose

   Bacterial expression of recombinant Arabidopsis thaliana GUN1 (amino acids 232 to 918)

   Depositor

   Tatsuru Masuda

   Article

   Shimizu et al Proc Natl Acad Sci U S A. 2019 Dec 3;116

   Insert

   cDNA of Arabidopsis thaliana GUN1 corresponding to amino acids 232 to 918 (GUN1 Mustard Weed)
   

   Use

   Tags

   TRX-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAA3

   Plasmid

   #128280

   Purpose

   asymmetric plasmid partitioning E. coli - target plasmid

   Depositor

   Matthew Bennett

   Article

   Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

   Insert

   Piq - mRFP, parS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-Cut-P(11)4

   Plasmid

   #185785

   Purpose

   Encodes enzyme cutinase linked to P(11)4 self-assembling peptide via a GS-linker sequence

   Depositor

   Lizhong He

   Article

   Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

   Insert

   Cut-P(11)4
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET26b-Granzyme A (S-A)

   Plasmid

   #8825

   Depositor

   Judy Lieberman

   Article

   Beresford et al Proc Natl Acad Sci U S A 1997 Aug 19;94(

   Insert

   Human Granzyme A inactive (GZMA Human)
   

   Use

   Tags

   (His)6

   Expression

   Bacterial

   Mutation

   changed Serine 184 to Alanine

   Promoter

   Available since

   June 22, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pTU398

   Plasmid

   #178980

   Purpose

   13S-S Candidatus “Scalindua brodae” Twin- Strep SUMO tagged RAMP (codon-optimization)

   Depositor

   Stan Brouns

   Article

   van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

   Insert

   gRAMP
   

   Use

   Tags

   Strep-Tag II SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. B7-5

    Plasmid

    #111493

    Purpose

    translational initiation factor 1

    Depositor

    Cheemeng Tan

    Article

    Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

    Insert

    translational initiation factor 1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. csr-1_hH1_mEos3.1_V5

    Plasmid

    #191750

    Purpose

    Photoconvertible fluorescent nuclear reporter.

    Depositor

    Jay C Dunlap

    Article

    Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

    Insert

    hH1 (hH1 Neurospora crassa)
    

    Use

    Tags

    V5, ccg-1 promoter, and mEos3.1

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAcCAST-sgRNA_entry (CJT83)

    Plasmid

    #181785

    Purpose

    Expresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AcTnsB, AcTnsC, AcTniQ, AcCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac and J23119

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a(+)/FaGH16A

    Plasmid

    #100910

    Purpose

    Expression of the Beta-1,3-1,4-Endo-Laminarinase FaGH16A in E.coli

    Depositor

    Jan-Hendrik Hehemann

    Article

    Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

    Insert

    FaGH16A
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET23a-His-hRanGAP1tail

    Plasmid

    #53139

    Purpose

    E.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tag

    Depositor

    Frauke Melchior

    Article

    Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

    Insert

    RanGAP1 (RANGAP1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C-terminal fragment (amino acids 398-587)

    Promoter

    T7

    Available since

    May 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pST-Ki

    Plasmid

    #44563

    Purpose

    for integrative constitutive expression of mycobacterial proteins

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBAD-HaloGFP-Na2.4

    Plasmid

    #228470

    Purpose

    Bacterial expression of green fluorescent sodium ion indicator HaloGFP-Na2.4

    Depositor

    Robert Campbell

    Article

    Takeuchi et al RSC Chem Biol. 2024 Dec 3. doi: 10.1039/

    Insert

    HaloGFP-Na2.4
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQLinkHD_ECFP

    Plasmid

    #118857

    Purpose

    fluorescent protein expression with an N-terminal His-tag

    Depositor

    Marc Birtwistle

    Article

    Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

    Insert

    ECFP
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSR357.24

    Plasmid

    #125109

    Purpose

    Expression of narL(1-159aa)-ydfI(153-213aa) chimera under PLtetO-1, output Promoter PydfJ115

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    narL(REC)-ydfI(DBD)159
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET23b-Casp2-Delta Card

    Plasmid

    #11810

    Depositor

    Guy Salvesen

    Insert

    Caspase-2 Delta Card (CASP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Delta Card

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

20. (SUMO)5-(SIM)10

    Plasmid

    #126923

    Purpose

    Bacterial expression plasmid for multivalent scaffold of (SUMO)5-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    5 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    internal BamHI site between (SUMO)5 and (SIM)10

    Promoter

    tac

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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