30,810 results

1. pCOLA banana sensor sfGFP

   Plasmid

   #107367

   Purpose

   Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified banana DNA

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   banana-detecting toehold + sfGFP
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PERK 5: PERK.K618R.pGEX4T-1

   Plasmid

   #21818

   Depositor

   David Ron

   Article

   Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

   Insert

   PERK (537-1114) K618R (Eif2ak3 Mouse)
   

   Tags

   GST and Myc (9E10)

   Expression

   Bacterial

   Mutation

   PERK Kinase Domain. Amino acids 537-1114. Kinase …

   Available Since

   March 29, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTSlb-N748D

   Plasmid

   #60732

   Purpose

   Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD. C-terminal fragment has the point mutations N748D.

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Inserts

   Residues 1-179 of split T7 RNAP
   Residues 180-880 of split T7 RNAP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Residues 1-180 of split T7 RNAP and Residues 180-…

   Available Since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCri-1b

   Plasmid

   #61327

   Purpose

   Protein expression.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

   Insert

   Green fluorescent protein
   

   Tags

   His-tag and MBP-tag

   Expression

   Bacterial

   Available Since

   Jan. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pProEx RhoA(G14C) CL

   Plasmid

   #224271

   Purpose

   Protein purification of RhoA (G14C)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   RhoA (RHOA Human)
   

   Expression

   Bacterial

   Mutation

   G14C

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pProEx Rheb(R15C) CL

   Plasmid

   #224266

   Purpose

   Protein purification of Rheb (R15C)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   Rheb (RHEB Human)
   

   Expression

   Bacterial

   Mutation

   R15C

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET14b-dabAB2

   Plasmid

   #133013

   Purpose

   pET14b carrying the dabA2 gene (Uniprot: D0KWS7) with a c-terminal strep tag fusion and the dabB2 gene (Uniprot: D0KWS8) with a c-terminal sfGFP V206K fusion and 6xHis tag

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Tags

   6xHis, StrepII tag, and sfGFP

   Expression

   Bacterial

   Promoter

   t7

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAraTMwt

   Plasmid

   #47514

   Purpose

   Contains A2B L980A TMCY mutant, expresses wt AraC for heterodimer competition assay

   Depositor

   Bryan Berger

   Article

   Su et al J Mol Biol. 2013 Jul 19. pii: S0022-2836

   Insert

   Integrin alpha2B L980A TM-CYTO
   

   Expression

   Bacterial

   Promoter

   Tac

   Available Since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBNG2

   Plasmid

   #149473

   Purpose

   Broad-Host range expression vector, pBBR origin, NahR(AM) repressor, PSalTTC promoter, GFPmut3b, Kanamycin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   NahR(AM)-GFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PSalTTC

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. GST-PRMT9 WT

    Plasmid

    #67607

    Purpose

    bacterial expression of wild type human PRMT9

    Depositor

    Steven Clarke

    Article

    Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

    Insert

    PRMT9 (PRMT9 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    wild type

    Promoter

    tac

    Available Since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSKDuet16

    Plasmid

    #41684

    Depositor

    Hideo Iwai

    Article

    Ellila et al FEBS Lett. 2011 Oct 10.

    Insert

    NpuDnaE intein
    

    Tags

    GB1 and His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAraGFP

    Plasmid

    #39548

    Depositor

    Bryan Berger

    Article

    Su et al J Biol Chem. 2012 Jul 22.

    Insert

    eGFP
    

    Expression

    Bacterial

    Available Since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. PGL4.11-COL1A1mSMAD

    Plasmid

    #230917

    Purpose

    Tested the COL1A1 promoter activity after abolition of the proximal SMAD binding sites in the promoter.

    Depositor

    Avi Rosenberg

    Article

    Yang et al Lab Invest. 2024 May;104(5):100336. doi:

    Insert

    Human COL1A1 promoter with mutant SMAD binding sequence (COL1A1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase-luc2p

    Expression

    Bacterial and Mammalian

    Mutation

    COL1A1 promoter with mutant potential SMAD bindin…

    Promoter

    COL1A1

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas3

    Plasmid

    #229538

    Purpose

    pMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Insert

    Cas3
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. MPRA Empty Vector

    Plasmid

    #204865

    Purpose

    Vector used for high-throughput cloning and subsequent transfection of MPRA libraries into mammalian cells.

    Depositor

    Philipp Maass

    Article

    Oliveros et al Cell Genom. 2023 May 24;3(7):100330. doi

    Type

    Empty backbone

    Expression

    Bacterial and Mammalian

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAD-mKate(KPS12)

    Plasmid

    #133831

    Purpose

    Fluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides.

    Depositor

    Oscar Kuipers

    Article

    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

    Insert

    mKate(KPS12)
    

    Use

    E.coli/bacillus spp. shuttle vector

    Expression

    Bacterial

    Promoter

    Pupp

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTriEx-mPA-GFP-PA-Rac1

    Plasmid

    #22022

    Purpose

    Expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1 (RAC1 Human, Avena sativa (oat))
    

    Tags

    6X His and mPA-GFP

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

18. 6CCR (GID4)

    Plasmid

    #119960

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    GID4 (C17orf39 Human)
    

    Tags

    6xHIS, thrombin,SUMO and TEV cleavage

    Expression

    Bacterial

    Mutation

    contains amino acids 116-300

    Promoter

    T7

    Available Since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pRU1099

    Plasmid

    #14464

    Depositor

    Philip Poole

    Article

    Karunakaran et al Microbiology. 2005 Oct . 151(Pt 10):3249

    Insert

    unstable gfp-mut3.1 AAV
    

    Expression

    Bacterial

    Available Since

    April 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET_SZ1-RGG-GFP-RGG

    Plasmid

    #124937

    Purpose

    Expression of SZ1-RGG-GFP-RGG

    Depositor

    Matthew Good, Daniel Hammer, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    SYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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