29,981 results

1. DP6

   Plasmid

   #140446

   Purpose

   Untargetted bacterial drift

   Depositor

   David Liu

   Article

   Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

   Insert

   dnaQ926 dam seqA emrR ugi CDA1 gIII
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. TccCas13a Twin SUMO

   Plasmid

   #199754

   Purpose

   Expresses codon optimized thermostabile TccCas13a in E.coli

   Depositor

   Magdy Mahfouz

   Article

   Mahas et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

   Insert

   TccCas13a
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   LacI

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. luxA+luxB/pRSETB Duet

   Plasmid

   #197581

   Purpose

   Expressing luxA and luxB under dual T7 promoter

   Depositor

   Takeharu Nagai

   Article

   Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

   Insert

   LuxA LuxB
   

   Use

   Tags

   T7 tag and 6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWZ853

   Plasmid

   #208115

   Purpose

   mSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-BSD

   Depositor

   Jef Boeke

   Article

   Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

   Insert

   mNeonGreen-HPRT1-BSD
   

   Use

   Tags

   T2A

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-MBP-mSA2

   Plasmid

   #52319

   Purpose

   Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as an MBP fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

   Depositor

   Sheldon Park

   Article

   Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

   Insert

   monomeric streptavidin 2
   

   Use

   Tags

   FLAG, His, MBP, and TEV

   Expression

   Bacterial

   Mutation

   S25H mutation compared to streptavidin

   Promoter

   T7

   Available since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. VR122 pETv2 10His-pG-Tn5 (E54K, L372P)

   Plasmid

   #198467

   Purpose

   E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in DH5α cloning strain.

   Depositor

   Viviana Risca

   Article

   Soroczynski et al bioRxiv 2024.07.11.602973

   Insert

   10His-pG-Tn5 (E54K, L372P)
   

   Use

   Tags

   10His-pG

   Expression

   Bacterial

   Mutation

   E54K, L372P

   Promoter

   Consensus φ10 T7 Promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. ER-GCaMP6f

   Plasmid

   #182548

   Purpose

   Expresses GCaMP6f on the cytosolic side of the ER (P-450site)

   Depositor

   Chris Richards

   Article

   Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

   Insert

   ER localized P450
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-EIN-FTO(32-505)

   Plasmid

   #186466

   Purpose

   Expresses human RNA demythelase protein FTO 32-505 amino acid sequence with N-terminal fusion with the N-domain of EI ( e.coli protein)

   Depositor

   Vincenzo Venditti

   Article

   Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055

   Insert

   Fat mass obesity associated protein (FTO Human)
   

   Use

   Tags

   6 Histidine-EIN (N-terminal domain of E.coli Enzy…

   Expression

   Bacterial

   Mutation

   amino acid 32-505

   Promoter

   T7

   Available since

   June 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLacO-ISce1

   Plasmid

   #58505

   Purpose

   provides 256 Lac operator repeats adjacent to the ISce1 restriction site

   Depositor

   Tom Misteli

   Article

   Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

   Insert

   Lac repeats adjacent to 6 copies of the ISce1 restriction site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   no promoter

   Available since

   Oct. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBad-HisB-RRvT

    Plasmid

    #87364

    Purpose

    Expresses RRvT, a tandem heterodimer red fluorescent protein

    Depositor

    Robert Campbell

    Article

    Wiens et al Chembiochem. 2016 Oct 26. doi: 10.1002/c

    Insert

    RRvT
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    pBad (Arabinose)

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEVI(408)-ColE-iLight-msfGFP

    Plasmid

    #170268

    Purpose

    Expresses LexA_DBD-iLight-msfGFP under J23116 promoter and mCherry under ColE promoter with a LexA408 operator in bacteria.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    iLight
    

    Use

    Tags

    msfGFP

    Expression

    Bacterial

    Mutation

    To design iLight for expression in bacteria, a fu…

    Promoter

    pJ23116

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. spNW30

    Plasmid

    #173485

    Purpose

    Expresses spNW30 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spNW30
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28 His6-mEGFP-D4H

    Plasmid

    #226373

    Purpose

    Production of recombinant mEGFP-D4H, a fluorescent probe binding cholesterol

    Depositor

    Catherine Tomasetto

    Article

    Eichler et al Methods Mol Biol. 2025;2888:101-118. doi

    Insert

    D4H (pfoA Clostridium perfringens)
    

    Use

    Tags

    His6 and monomeric EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. KARS_pNIC-Bio3

    Plasmid

    #153051

    Purpose

    Expression construct for producing Avi tagged Lysyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    KARS (KARS1 Human)
    

    Use

    Tags

    Avi and His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pREDusk-MCS

    Plasmid

    #188970

    Purpose

    For red light-repressed bacterial expression of gene of interest

    Depositor

    Heikki Takala

    Article

    Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC0059 PbuCas13 (B03) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115209

    Purpose

    Expresses PbuCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    PbuCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115216

    Purpose

    Expresses Pin2Cas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    Pin2Cas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pC0058 PinCas13 (B02) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115208

    Purpose

    Expresses PinCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    PinCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BirA in pET28a (w400-2)

    Plasmid

    #26624

    Depositor

    Eric Campeau

    Insert

    BirA
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115207

    Purpose

    Expresses BzoCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    BzoCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge