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Showing: 741 - 760 of 29557 results

  8. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet3

    Plasmid
    #189752
    Purpose
    Produces an mTurquoise-antiFRET-mScarlet3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet3 FPs for ratiometric analysis
    Depositor
    Dorus Gadella
    Article
    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi
    Insert
    mTurquoise2_spatial-linker-P2A_mScarlet3
    Use
    Tags
    6xHis
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV & Rha
    Available since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    189752-plasmid-map-sequence-id-375290

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  15. pBBR-Cas9ΔyqhD-HA500

    Plasmid
    #220369
    Purpose
    A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440
    Depositor
    Yilan Liu
    Inserts
    SpCas9
    sgRNA for yqhD in P. putida KT2440
    Use
    CRISPR; Broad host range
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    220369-plasmid-map-sequence-id-437258

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  16. pET-28b-T7-henAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1935)

    Plasmid
    #114073
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a-HF1(E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
    Depositor
    Benjamin Kleinstiver, Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R) with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, K548R and N282A
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114073-plasmid-map-sequence-id-220529

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  20. pGMC00025 (aka pCW-dCas9-Blast)

    Plasmid
    #195316
    Purpose
    Dox inducible lentiviral dead Cas9
    Depositor
    Raj Chari
    Insert
    dead Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    D10A; H840A
    Promoter
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    195316-plasmid-map-sequence-id-385750

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Showing: 741 - 760 of 29557 results