32,218 results
-
Plasmid#8754DepositorInsertBax (Bax Mouse)
ExpressionBacterialAvailable SinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
-
pHH0103_UBASH3B-1/1
Plasmid#91462PurposeProtein expression and purification of human SH3 domain construct UBASH3B-1/1DepositorInsertUBASH3B-1/1 (UBASH3B Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTJV1Sc2-tetA
Plasmid#166982PurposeGenerates ssDNA in vivo targeting tetA for recombineering w/ CspRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistanceDepositorInsertCspRecT
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterpVanCCAvailable SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtTcR1AtR2NtB2
Plasmid#160889PurposeExpression of N. tabacum Rubisco small subunit (rbcS-Tc), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-Tc
ExpressionBacterialPromoterrbcS-Tc: T7, chaperones: T12Available SinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
RBM23_2xRRM_pGEX
Plasmid#135118PurposeRecombinant expression of RBM23 with dual-affinity tagDepositorAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
SpIMPDH
Plasmid#206980PurposeStreptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.DepositorInsertSpIMPDH (guaB Streptococcus pyogenes)
TagsHis tag (with TEV cleavage site)ExpressionBacterialAvailable SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
T7RNAP Cyslight A188C
Plasmid#205083PurposeT7RNAP protein expressionDepositorInsertT7RNAP Cyslight A188C (T7p07 Bacteriophage T7)
ExpressionBacterialMutationC125S, C347S, C510S, C515S, C530S, C723S N88CAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-NEMOD311N
Plasmid#11968DepositorAvailable SinceJuly 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEX-EGFAB(H306Y)
Plasmid#25771DepositorAvailable SinceDec. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
OR gate
Plasmid#44451DepositorInsertOR-gate
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCVD438
Plasmid#63759Purposeeae gene (3476 bp) from pJY21 removed by SamI/SphI digestion, cloned into EcoRV/SphI sites of pACYC184. Insertional inactivation of tetracycline gene.DepositorInserteae
ExpressionBacterialAvailable SinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
GMPSA
Plasmid#42402PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
HM3C NaVAe1_p pET24b
Plasmid#127854PurposeBacterial expression vector for NaVAe1 bacterial sodium channel residues 143-288. It expresses HIStag-MBP-3C-channel protein.DepositorInsertNaVAe1p
Tags3C Cleavage site, His, and MBPExpressionBacterialAvailable SinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
S1-71 pComb3xss anti-cFos nanobody
Plasmid#145832PurposeBacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-cFos (Human) recombinant llama nanobody S1-71 (FOS L. glama (llama))
Tags6xHis-HAExpressionBacterialPromoterlacAvailable SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBV58
Plasmid#204603PurposeERG11-3X Minidegron-5X FLAG-HIS3MX6DepositorAvailable SinceJuly 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph
Plasmid#218366PurposeEmpty backbone for Tn7-site insertionDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMApra-Ptol
Plasmid#218359PurposeEmpty vector with XylS-Pm expression systemDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC18T-mini-Tn7T-hph-Ptol
Plasmid#218369PurposeEmpty backbone for Tn7-site insertion with XylS-Pm expression systemDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGST2-ALIX (795-811)
Plasmid#21502DepositorAvailable SinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046879
Plasmid#149238PurposeBacterial expression plasmid for SARS-CoV-2 nsp2DepositorInsertSARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceMay 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX-HCF-1rep1E10A
Plasmid#84607PurposePlasmid contains HCF-1rep1E10A with GST-tagDepositorAvailable SinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-HCF-1rep1wt
Plasmid#84606PurposePlasmid contains HCF-1rep1wt with GST-tagDepositorAvailable SinceSept. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKD317.8
Plasmid#181951PurposeIPTG-inducible expression of NarXDepositorExpressionBacterialPromoterNarX-Ptac; lacI-PlacIqAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCh-LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)
Plasmid#165030PurposeExpress cpFRB2-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherryDepositorInsertLaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)
TagsmCherryExpressionBacterial and MammalianPromoterT7Available SinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
HARS_pNIC-Bio3
Plasmid#153050PurposeExpression construct for producing Avi tagged histidyl tRNA synthetaseDepositorAvailable SinceJune 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMRE166
Plasmid#118514Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmCardinal
ExpressionBacterialAvailable SinceNov. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-T5-FB-E25TAG
Plasmid#176545PurposeExpresses IgG affinity protein FB with a TAG stop codon at E25 positionDepositorInsertIgG affinity protein
TagsHis tagExpressionBacterialMutationchange glutamic acid 25 to amber stop codonPromoterT5Available SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDHK29
Plasmid#80783PurposeCloning vector compatible with ColE1 plasmids with high copy numberDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTac-E-luci
Plasmid#65937PurposeTheophylline Riboswitch E with luciferase geneDepositorInsertluciferase
ExpressionBacterialAvailable SinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only