30,875 results

1. pPbuCas13b

   Plasmid

   #184565

   Purpose

   Inducible expression of PbuCas13b nuclease in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Cas13b nuclease from Prevotella buccae
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Nov. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPINMSYB

   Plasmid

   #41118

   Depositor

   Ray Owens

   Article

   Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

   Type

   Empty backbone

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   March 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST-GST-FBW7 alpha

   Plasmid

   #197456

   Purpose

   The plasmid expresses GST-tagged FBW7 human isoform 1. Used for in vitro translation of FBW7alpha.

   Depositor

   Omar Khan

   Article

   Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

   Insert

   FBW7 (FBXW7 Human)
   

   Use

   In vitro translation

   Tags

   GST

   Expression

   Bacterial

   Mutation

   See depositor comments below

   Promoter

   T7

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFosill-2

   Plasmid

   #89696

   Purpose

   to make Fosmids that can be converted into Illumina sequencable libraries

   Depositor

   Andreas Gnirke

   Article

   Williams et al Genome Res. 2012 Nov;22(11):2241-9. doi:

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET His6 Mocr TEV LIC cloning vector (2O-T)

   Plasmid

   #29710

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   His6-Mocr-TEV

   Expression

   Bacterial

   Available Since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMS20: pHelper(PmcCAST)_PmcPSP1_ΔTnsD

   Plasmid

   #168153

   Purpose

   Inducible expression of PmcCAST proteins (except TnsD) with crRNA targeting PmcPSP1.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   PmcCAST minimal CRISPR array (with spacer for PmcPSP1)
   PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   PmcCAST Tns proteins (TnsAB and TnsC)
   PmcTniQ
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEB2_mScarletI_mVenusNB-MrkHR113A

   Plasmid

   #182292

   Purpose

   expresses c-di-GMP mutant biosensor in E. coli

   Depositor

   Howard Berg

   Article

   Vrabioiu et al Proc Natl Acad Sci U S A. 2022 Feb 8;119

   Insert

   mVenusNB-MrkHR113A
   

   Tags

   mVenusNB internal fusion to MrkH with a R113A poi…

   Expression

   Bacterial

   Mutation

   changed arginine 113 to alanine, MrkH numbering …

   Promoter

   proC

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMS13: pTarget(AvCAST)

   Plasmid

   #168146

   Purpose

   Target plasmid containing AvPSP1 and attachement site for AvCAST.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   PAM for AvCAST + AvPSP1
   glmS
   

   Expression

   Bacterial

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-Octo_VirD2_NLS-Cas9-6xHis

   Plasmid

   #232296

   Purpose

   Expresses Cas9 protein fused with octopine-type VirD2-originating NLS

   Depositor

   Hee-Sik Kim

   Article

   Le et al Proc Natl Acad Sci U S A. 2025 Mar 11;12

   Insert

   Octo_VirD2_NLS-Cas9-6xHis
   

   Use

   CRISPR

   Tags

   6xHis and Octopine-type VirD2-derived NLS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAB202

    Plasmid

    #101674

    Purpose

    Opto-T7RNAP(563)

    Depositor

    Mustafa Khammash

    Article

    Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

    Insert

    Opto-T7RNAP(563)
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET22-His-TEV-DOT1L

    Plasmid

    #89610

    Purpose

    Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L residues 1-416

    Depositor

    Leemor Joshua-Tor

    Article

    Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

    Insert

    Dot1L (DOT1L Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUCBB-pT7-eGFP

    Plasmid

    #32552

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Bacterial

    Promoter

    pT7

    Available Since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEB1-coSCFP3A

    Plasmid

    #104015

    Purpose

    Plasmid encoding coSCFP3A

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    coSCFP3A
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Same protein sequence as SCFP3A (codon optimized …

    Promoter

    proC

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBAD-Citrine2

    Plasmid

    #110772

    Purpose

    Yellow fluorescent protein with improved photostability

    Depositor

    Robert Campbell

    Article

    Wiens et al Integr Biol (Camb). 2018 Jul 16;10(7):41

    Insert

    Citrine2
    

    Tags

    His tag

    Expression

    Bacterial

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. csr-1_hH1_mEos3.1_V5

    Plasmid

    #191750

    Purpose

    Photoconvertible fluorescent nuclear reporter.

    Depositor

    Jay C Dunlap

    Article

    Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

    Insert

    hH1 (hH1 Neurospora crassa)
    

    Tags

    V5, ccg-1 promoter, and mEos3.1

    Expression

    Bacterial

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKEW212-MBP-TEV-AcrVA4

    Plasmid

    #115658

    Purpose

    Expresses AcrVA4 in bacteria via T7 RNAP, for purification

    Depositor

    Jennifer Doudna

    Article

    Watters et al Science. 2018 Oct 12;362(6411):236-239.

    Insert

    AcrVA4
    

    Tags

    MBP-TEV

    Expression

    Bacterial

    Promoter

    T7 RNAP

    Available Since

    Oct. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPBC-LG3-tdT

    Plasmid

    #62810

    Purpose

    pPBC-LG3-tdT expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP3 and tdTomato under the control of the CAG promoter.

    Depositor

    Karen Wilcox

    Article

    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

    Insert

    ITR-CAG-Lck-GCaMP3-IRES-tdTomato-ITR
    

    Expression

    Bacterial and Mammalian

    Promoter

    CAG

    Available Since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-mBoNT/F

    Plasmid

    #168015

    Purpose

    Expresses MBP-BoNT/F-LC(1-428)-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    MBP-BoNT/F-LC(1-428)-6xHis
    

    Tags

    Hisx6 and maltose binding protein

    Expression

    Bacterial

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. SOCS4B-c002

    Plasmid

    #53669

    Purpose

    bacterial expression plasmid

    Depositor

    Nicola Burgess-Brown

    Insert

    SOCS4B
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    Bacterial expression for structure determination;…

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pET28_6xHis-PARP1delCAT

    Plasmid

    #173942

    Purpose

    Bacterial expression of PARP1 lacking CAT domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delCAT (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 661-1014

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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