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pDB.His.MBP.3C CARD8 CARD WT

Plasmid

#164022

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 UPA-CARD

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Tags

His6-MBP-3C

Expression

Bacterial

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pPSV39-dddAI

Plasmid

#186557

Purpose

Broad host range regulated expression of the DddA immunity determinant (DddAI)

Depositor

Joseph Mougous

Article

Gallagher et al Nat Microbiol. 2022 Jun;7(6):844-855. do

Insert

dddAI

Expression

Bacterial

Promoter

lacUV5

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA

Plasmid

#64973

Purpose

Expresses the wild type of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

T7 promoter

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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HS-aPKC-CAAX-mKate2

Plasmid

#105950

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

aPKC-CAAX

Tags

mKate2

Expression

Bacterial

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX PLCg1(NC)-SH2

Plasmid

#46471

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

PLCg1(NC) (PLCG1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 542-759

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2-mkgDUX4

Plasmid

#21156

Depositor

Stephen Tapscott

Article

Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

Insert

DUX4 (DUX4 Human)

Expression

Bacterial and Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pMRE154

Plasmid

#118504

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mOrange2

Expression

Bacterial

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAB092a

Plasmid

#79217

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2) gIII, luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1+2); Ppro1

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pHflu4

Plasmid

#203882

Purpose

pHI1 with insertion of monomeric red fluorescent protein (mRFP)

Depositor

Bogumil Karas

Insert

monomeric red fluorescent protein

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pC028 - LbfCas13a from Listeriaceae bacterium FSL M6-0635

Plasmid

#91918

Purpose

Expresses LbfCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LbfCas13a and guide expression cassette

Expression

Bacterial

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pBTK1006 (pSL5)

Plasmid

#191001

Purpose

Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

Depositor

Jeffrey Barrick

Article

Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

Insert

red chromoprotein

Use

Synthetic Biology

Expression

Bacterial

Promoter

CP25

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase

Plasmid

#196185

Purpose

Co-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-His

Depositor

Jesse Zalatan

Article

Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

Inserts

GSK3B (GSK3B Human)
λPPase

Tags

GST, HA, His6, and MBP

Expression

Bacterial

Mutation

changed Serine 9 to Alanine

Promoter

Tac

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAGD289

Plasmid

#228113

Purpose

pET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter

Depositor

Seth Shipman

Article

Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

Insert

Kva1 retron, ncRNA with 90 bp rpoB donor

Expression

Bacterial

Promoter

T7

Available Since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCOLA banana sensor sfGFP

Plasmid

#107367

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified banana DNA

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

banana-detecting toehold + sfGFP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCPP5060

Plasmid

#128716

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

Depositor

Alan Collmer

Article

Lopez-Solanilla et al Mol Microbiol. 2004 Oct;54(2):353-65. do

Insert

shcN-hopN1

Expression

Bacterial

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCPP6450

Plasmid

#128731

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Article

Wei et al Cell Host Microbe. 2015 Jun 10;17(6):752

Insert

HopAD1

Expression

Bacterial

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pETDuet-AtRBX1-T7-AtSKP1-T7-AtCUL1-S

Plasmid

#238003

Purpose

Expression AtRBX1-T7, AtSKP1-T7 and AtCUL1-S in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Inserts

AtRBX1 (RBX1 Mustard Weed)
AtSKP1 (SKP1 Mustard Weed)
AtCUL1 (CUL1 Mustard Weed)

Tags

S and T7

Expression

Bacterial

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1125

Plasmid

#235742

Purpose

Protein expression of GST-tagged PX domain (Cys at 2) in bacteria

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

p40 PX domain (NCF4 Human)

Tags

GST-TEV

Expression

Bacterial

Mutation

aa 2-149 with Cys at 2

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGal-Trans-Myc-eGFP-KDEL

Plasmid

#177729

Purpose

ER protein collagen beta (1-O) galactosyltransferase fused to eGFP at the C-terminus, myc tagged

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

collagen beta(1-O)galactosyltransferase 1 (COLGALT1 Human)

Tags

KDEL, Myc, and eGFP

Expression

Bacterial and Mammalian

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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R701-X72-527: His6-tev-Hs.CALM1(76-148)

Plasmid

#159695

Purpose

E. coli protein expression of His6-tev-Hs.CALM1(76-148)

Depositor

Dominic Esposito

Article

Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

Insert

His6-tev-Hs.CALM1(76-148) (CALM1 Human)

Tags

His6-tev

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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