30,180 results

1. pGBW-m4046814

   Plasmid

   #145601

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   Escherichia coli recode 1

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pQO18-TEV

   Plasmid

   #27399

   Depositor

   Jonathan Javitch

   Article

   Zhao et al Nature. 2010 May 13. 465(7295):188-93.

   Insert

   leuT
   

   Use

   Tags

   HisX10 and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. GLI-3 bs-2

   Plasmid

   #16420

   Depositor

   Bert Vogelstein

   Article

   Kinzler et al Nature. 1988 Mar 24. 332(6162):371-4.

   Insert

   GLI3 (GLI3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 10, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pGEX-5X-1 U1-C-FL

   Plasmid

   #129077

   Purpose

   generation of GST-tagged U1-C protein using bacteria

   Depositor

   Takeshi Tsubata

   Insert

   U1-C (SNRPC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBYK01

   Plasmid

   #138656

   Purpose

   phiOZJ integrating Streptomyces expression vector lacking RDF

   Depositor

   Joshua Blodgett

   Article

   Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PermE* variant sequence from pDA1652

   Available since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Osa-pCAMBIA-1301-STTM319

   Plasmid

   #84201

   Purpose

   Disturb the expression of miR319 in rice

   Depositor

   Guiliang Tang

   Article

   Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

   Insert

   miR319
   

   Use

   Tags

   Expression

   Bacterial, Plant, and Yeast…

   Mutation

   Promoter

   2x35S

   Available since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pE3n

   Plasmid

   #17457

   Purpose

   modified pENTR vector for N-terminal 6x MYC tag fusion with your gene of interest

   Depositor

   Giovanna Benvenuto

   Article

   Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

   Type

   Empty backbone

   Use

   Modified gateway entry vector

   Tags

   6xMyc

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   Promoter

   Available since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a(ICOS)

   Plasmid

   #177903

   Purpose

   Bacterial expression of his10-tagged human ICOS Intracellular Domain

   Depositor

   Ron Vale

   Article

   Hui et al Science. 2017 Mar 31;355(6332):1428-1433

   Insert

   ICOS (aa 162-199 only) (ICOS Human)
   

   Use

   Tags

   10xHis-Thrombin cut site-T7 tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. proinsulin-msGFP2 (C48S C70S)

   Plasmid

   #218956

   Purpose

   Expresses proinsulin fused to msGFP2 (C48S C70S) in bacteria

   Depositor

   Benjamin Glick

   Insert

   proinsulin-msGFP2 C48S C70S
   

   Use

   Tags

   proinsulin

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. BbZIP-cALFA

    Plasmid

    #160498

    Purpose

    For the protein expression of BbZIP with ALFA tag at C-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Insert

    BbZIP
    

    Use

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pK27Sumo_His-SUMO-nsp14-nsp10 fusion (SARS-CoV-2)

    Plasmid

    #169160

    Purpose

    To express nsp14-nsp10 fusion protein in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    14His-SUMO-nsp14-GGSGGS-nsp10 (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    14His-SUMO and Fusion protein of SARS-CoV-2 nsp14…

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T5

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. His6-Twin-Strep-SUMO-pET28a-EfaCas2

    Plasmid

    #112794

    Purpose

    To express enterococcus faecalis Cas2 protein in E. coli

    Depositor

    Ailong Ke

    Article

    Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

    Insert

    EfaCas2
    

    Use

    Tags

    His6-Twin-Strep-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28b::mAID

    Plasmid

    #15923

    Depositor

    Nina Papavasiliou

    Article

    Besmer et al Mol Cell Biol. 2006 Jun . 26(11):4378-85

    Insert

    AID (Aicda Mouse)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPR_eGFP_497

    Plasmid

    #111824

    Purpose

    Knockout eGFP

    Depositor

    Steve Shih

    Article

    Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

    Insert

    gRNA targeting eGFP 477-497
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOSIP-TH (TetR, HK022)

    Plasmid

    #45988

    Depositor

    Keith Shearwin, Drew Endy

    Article

    St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBEL1092

    Plasmid

    #195635

    Purpose

    Expression/purification of 6xHis-TEV-sfGFP

    Depositor

    Gira Bhabha, Damian Ekiert

    Article

    MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GST-AR-1-333

    Plasmid

    #104194

    Purpose

    Expresses human AR aa 1-333 in pGEX-5x-1 bacteral expression vector with GST

    Depositor

    Elizabeth Wilson

    Article

    He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

    Insert

    human androgen receptor aa 1-333 (AR Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYTK036

    Plasmid

    #65143

    Purpose

    Encodes Cas9 as a Type 3 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21-pelB-LaG-26

    Plasmid

    #172751

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-26; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-26
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFPC1-EPSIN 2

    Plasmid

    #22242

    Depositor

    Pietro De Camilli

    Article

    Rosenthal et al J Biol Chem. 1999 Nov 26. 274(48):33959-

    Insert

    Epsin 2 (EPN2 Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only