33,128 results
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Plasmid#115653PurposeExpresses MbCas12a in bacteria from M. bovoculi strain 58069, under TetR controlDepositorInsertMbCas12a
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPtetAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPS0254
Plasmid#115506PurposeReporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing celBDepositorInsertRlv3841pNifH pOGG082, celB pOGG050, T-pharma pOGG003 assembled in pOGG026
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCOLA kiwi sensor sfGFP
Plasmid#107368PurposeExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified kiwi DNADepositorInsertkiwi-detecting toehold + sfGFP
ExpressionBacterialPromoterT7Available SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTHSSe_40
Plasmid#109239PurposePTac driving TALEsp2DepositorInsertTALEsp2
ExpressionBacterialPromoterPTacAvailable SinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-TrxRFP1
Plasmid#115327PurposeBacterial expression of TrxRFP1DepositorInsertTrxRFP1
TagsHis-TagExpressionBacterialPromoterpBADAvailable SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
ath5:GFP-CAAX
Plasmid#105958Purposetransgenesis, EGFP membraneDepositorInsertCAAX
TagsGFPExpressionBacterialAvailable SinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_S-Luc_CLIP
Plasmid#113923PurposePlasmid encoding the bacterial expression of the chimeric SNAP-NanoLuc fusion dubbed S-Luc c-terminally linked to CLIP-tag for BRETDepositorInsertS-Luc_CLIP
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
B6-1
Plasmid#111485PurposePhe-tRNA synthetase BDepositorInsertPhe-tRNA synthetase B
Tags6xHisExpressionBacterialAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
B3-1
Plasmid#111471Purposetranslational initiation factor 2DepositorInserttranslational initiation factor 2
Tags6xHisExpressionBacterialAvailable SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
B4-5
Plasmid#111479Purposeribosome recycling factorDepositorInsertribosome recycling factor
Tags6xHisExpressionBacterialAvailable SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-omega
Plasmid#114632PurposeAddgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAPDepositorInsertdCas9-omega
UseCRISPRExpressionBacterialPromoterTet promoterAvailable SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-PTS
Plasmid#108953Purposeheterologous expression of PTS of P. cablin in E. coliDepositorInsertPatchoulol synthase (PTS)
TagsHis6-TagExpressionBacterialAvailable SinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
TJP1_PDZ_1
Plasmid#103909PurposeProtein expression and purification of ZO1 PDZ1 domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
TJP1_PDZ_2
Plasmid#103910PurposeProtein expression and purification of ZO1 PDZ2 domainDepositorAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 NEDD4L WW domain #3
Plasmid#104206PurposeBacterial expression of WW domain #3 from NEDD4LDepositorInsertNEDD4L WW domain #3 (NEDD4L Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAT5
Plasmid#112586PurposeUsed for expression of protein in E. coli as non-cleavable C-terminal 6His-tag fusionsDepositorTypeEmpty backboneTags6HisExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP3GT
Plasmid#112607PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and GST fusionsDepositorTypeEmpty backboneTagsAvi - 8His-GSTExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAT4
Plasmid#112580PurposeUsed for expression of protein in E. coli as non-fusionsDepositorTypeEmpty backboneExpressionBacterialAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJB02
Plasmid#111959PurposeExpresses GST-GFP-DHFR; construct generated for pull-down and localization experimentsDepositorInsertsmEGFP
eDHFR
TagsDHFRExpressionBacterialMutationeDHFR;residues 2-159 and enhanced GFP with monome…PromoterT7Available SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBTK509
Plasmid#110610PurposeBTK assembled broad-host-range plasmid encoding GFP driven by PA2 promoterDepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 21, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits