30,875 results

1. pPBC-LG6f

   Plasmid

   #62808

   Purpose

   pPBC-LG6f expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6f under the control of the CAG promoter.

   Depositor

   Karen Wilcox

   Article

   Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

   Insert

   ITR-CAG-Lck-GCaMP6f-ITR
   

   Expression

   Bacterial and Mammalian

   Promoter

   CAG

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIB190

   Plasmid

   #90197

   Purpose

   E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

   Depositor

   Indranil Biswas

   Article

   Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

   Type

   Empty backbone

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHis2-Arf1 (17-181)-Q71L

   Plasmid

   #184808

   Purpose

   Bacterial expression of Arf1 (aa17-181, Q71L mutation)

   Depositor

   James Hurley

   Article

   Ren et al Cell. 2013 Feb 14;152(4):755-67. doi: 10

   Insert

   Arf1 (ARF1 Human)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   aa 17-181 only, Q71L

   Promoter

   T7

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMal-Halo

   Plasmid

   #209349

   Purpose

   Protein expression vector with a TEV cleavable N-terminal 6xHis-MBP and a HRV 3C cleavable C-terminal HaloTag-StepTagII

   Depositor

   Lucia Strader

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Fusion of Dengue2 NS2B-NS3

   Plasmid

   #204800

   Purpose

   Bacterial expression of a fusion of Dengue2 NS2B-NS3 proteins

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Dengue2 NS2B-NS3 fusion
   

   Expression

   Bacterial

   Available Since

   Dec. 11, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pJUMP18-Histag_C

   Plasmid

   #127035

   Purpose

   Basic Part C- C-terminus; polyhistidine tag

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Insert

   Part His6_C
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   None

   Available Since

   July 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221-SARA-no stop

   Plasmid

   #67347

   Purpose

   hSara without stop codon in Gateway Entry vector

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   SARA (SAR1B Human)
   

   Use

   Gateway entry vector

   Expression

   Bacterial

   Promoter

   None

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

8. KRigor980

   Plasmid

   #129763

   Purpose

   Expresses full-length Drosophila Kinesin-1 with rigor mutation (R210A) for adhering microtubules to surfaces

   Depositor

   William Hancock

   Insert

   KHC (Khc Fly)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   R210A

   Available Since

   Nov. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. B1-3

   Plasmid

   #111465

   Purpose

   translational release factor 3

   Depositor

   Cheemeng Tan

   Article

   Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

   Insert

   translational release factor 3
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-Tight-Puro_ST18

    Plasmid

    #64844

    Purpose

    A lentiviral vector for the expression of mouse ST18 under doxycyline control

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    ST18 (St18 Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTD-C_sfYFPTwinStrep

    Plasmid

    #45943

    Purpose

    expresses P.putida codon optimized super-folder sfYFP-Twin-Strep fusion protein

    Depositor

    Thorben Dammeyer

    Article

    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

    Insert

    synthetic sfYFP (codon usage adapted to P.putida KT2440)
    

    Tags

    Twin-Strep-tag

    Expression

    Bacterial

    Promoter

    lacIq/Ptrc

    Available Since

    Sept. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-KG-CAND1

    Plasmid

    #19949

    Depositor

    Yue Xiong

    Article

    Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

    Insert

    CAND1 (cullin-associated and neddylation-dissociated 1) (CAND1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pBAT5

    Plasmid

    #112581

    Purpose

    Used for expression of protein in E. coli as non-fusions without initiation ATG

    Depositor

    Marko Hyvönen, Johan Peränen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC57-codon-modified HTK

    Plasmid

    #34563

    Depositor

    David Anderson

    Article

    Lo et al Neuron. 2011 Dec 22;72(6):938-50.

    Insert

    codon-modified HTK
    

    Expression

    Bacterial

    Mutation

    codon modified HTK using mouse codon usage table

    Promoter

    pUC57 bacteria promoter

    Available Since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. TBXTA-c028

    Plasmid

    #139765

    Purpose

    Protein expression/Streptavidin pull-down

    Depositor

    Opher Gileadi

    Insert

    TBXT, Full-length, WT (TBXT Human)
    

    Tags

    Avi-tag (Biotin) and His6-GST-TEV

    Expression

    Bacterial

    Mutation

    Contains only amino acids S2- M435

    Promoter

    T7

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. mTopaz-pBAD

    Plasmid

    #54841

    Purpose

    Localization: Protein Expression Vector , Excitation: 514, Emission: 527

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    6xHis and mTopaz

    Expression

    Bacterial

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

17. RAB25

    Plasmid

    #26163

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    RAB25:HPC038-B06:C25773 (RAB25 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pHEN2 F8-2

    Plasmid

    #186033

    Purpose

    Express VHH F8-2 with 3 c-myc tag in E. coli periplasm

    Depositor

    Isabelle Landrieu

    Article

    Danis et al Mol Ther. 2022 Apr 6;30(4):1484-1499. do

    Insert

    VHH F8-2
    

    Tags

    6His and Myc

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX6P1-DEST-FLAG

    Plasmid

    #119754

    Purpose

    Allows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained C-terminal FLAG tag.

    Depositor

    Andrew Jackson, Martin Reijns

    Type

    Empty backbone

    Tags

    FLAG and GST

    Expression

    Bacterial

    Promoter

    tac promoter

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAWP78-PVCpnf_pvc13-A4DARPin

    Plasmid

    #198288

    Purpose

    Produces PVCs retargeted with anti-lysozyme DARPin A4

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-A4DARPin
    

    Expression

    Bacterial

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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