32,947 results
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Plasmid#102543PurposeBacterial expression of sepHluorin fused to the cell penetrating peptide TAT with a GST tag for purificationDepositorInsertTat-sepHluorin
TagsGSTExpressionBacterialPromotertacAvailable SinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDB036d
Plasmid#99216PurposeAccessory plasmid for PACE of MjTyrRS variants; negative selectionDepositorInsertPpsp [SD8] gIII; PproK tyrT(Opt,CUA); PproA [SD4] T7RNAP(S12*,S203*)
ExpressionBacterialMutationamber codons at positions 12 and 203 of T7 RNA po…PromoterPpsp, PproK, and PproAAvailable SinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETDuet RagA-RagC(D181N)
Plasmid#99643PurposeBacterial expression of RagA-RagC(D181N)DepositorAvailable SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAP974-1
Plasmid#99497PurposeTEV::linker::tagRFP::3XFlagDepositorInsertTEV::linker::tagRFP::3XFlag
ExpressionBacterialAvailable SinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mEos3.1 (mEosFP3.1-A69T)
Plasmid#99221PurposeBacterial expression of the primed conversion capable protein pr-mEos3.1 (mEos3.1-A69T).DepositorInsertpr-mEos3.1
ExpressionBacterialMutationA69T for primed conversion applicationsPromoterT7Available SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-ffDronpa-F
Plasmid#101193PurposeA fast switching Dronpa variantDepositorInsertffDronpa-F
Tags6xHis, T7 tag, and Xpress tagExpressionBacterialMutationK45I, V60A, F173VPromoterT7Available SinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXPA-fapO-RgTALsyn
Plasmid#100946PurposeExpression of Tyrosine Ammonia Lyase from constitutive GAP promoterDepositorInsertRgTALsyn
UseSynthetic BiologyExpressionBacterialMutationSilent mutation to remove internal NdeI site on T…PromoterGAP (Constitutive)Available SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
Keasling-2337
Plasmid#100168PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertlacIq-Ptrc-ADS
ExpressionBacterialPromotertrcAvailable SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
Keasling-2187
Plasmid#100167PurposeUsed to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growthDepositorInsertMevT and MBIS operon
ExpressionBacterialPromoterlacUV5Available SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRIM33 (TRIM33B-c100)
Plasmid#98243PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTRIM33B (TRIM33 Human)
TagsHis-6-TEVExpressionBacterialMutationpfam00628:PHD 889-932, pfam00439:Bromodomain 963-…PromoterT7Available SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
CFL1 (CFL1A-c002)
Plasmid#98237PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCFL1A (CFL1 Human)
TagsHis-6-TEVExpressionBacterialMutationpfam00241:Cofilin_ADF 27-148PromoterT7Available SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-NCBD + ACTR
Plasmid#99335PurposeBacterial coexpression of CBP NCBD and NCoA3 binding domainsDepositorExpressionBacterialPromoterT7Available SinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-beta2(616-951)
Plasmid#100743PurposeBacterial expression of GST-tagged beta2 subunit of AP2 complex (hinge and ear) long splice formDepositorAvailable SinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21-GFP-AURKA-mCherry
Plasmid#99876PurposeExpression of GFP-AURKA-mCherry for protein purification in bacteriaDepositorAvailable SinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT34
Plasmid#89669Purposeexpression of doubleTAL staple protein dT34 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT34
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEHIShqprt
Plasmid#92166PurposePlasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vectorDepositorInsertQuinolinate Phosphoribosyltransferase (QPRT Human)
TagsTEV protease -cleavable N-terminal 6HisExpressionBacterialMutationchanged Asp 2 to Asn to create BspHI sitePromoterT7Available SinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE60 Trx1 CSAAA
Plasmid#98401PurposeBacterial expression vector for Trx1 CSAAADepositorAvailable SinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
TDP-43_CTD_Q331K
Plasmid#98673PurposeExpresses 6xHis-tagged C-terminal domain of TDP-43 with Q331KDepositorAvailable SinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CohIII-CBM-HIS-LPETGG
Plasmid#91697PurposeCohIII-CBM handle with C-Terminal Sortase Recognition Sequence LPETGG for Posttranslational Coupling to a N-terminal GGGDepositorInsertCohIII-CBM-HIS-LPETGG
TagsHIS-Tag and Sortase-TagExpressionBacterialMutationCysteine deletion in CBM C63SPromoterT7Available SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_K1087A
Plasmid#91864PurposeBacterial expression for Cas13aDepositorInsertLbuCas13a (LbuC2c2) K1087A
TagsHis6-MBP-TEV siteExpressionBacterialMutationK1087AAvailable SinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only