29,996 results

1. pWUR702

   Plasmid

   #53079

   Purpose

   Inducible TtAgo expression

   Depositor

   John van der Oost

   Article

   Swarts et al Nature. 2014 Mar 13;507(7491):258-61. do

   Insert

   TtAgo
   

   Use

   Tags

   Strep-II tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pYES1L-URA

   Plasmid

   #84301

   Purpose

   E.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.

   Depositor

   George Church

   Article

   Ostrov et al Science. 2016 Aug 19;353(6301):819-22. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pdCas9-sgRNA-RFP

   Plasmid

   #166005

   Purpose

   Bacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)

   Depositor

   Martin Buck

   Article

   Bradley et al Biotechnol Rep (Amst). 2021 Oct 9;32:e00

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTP396

   Plasmid

   #149336

   Purpose

   Bacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobody

   Depositor

   Rebecca Voorhees

   Article

   Pleiner et al Science. 2020 May 21. pii: science.abb50

   Insert

   anti-GFP nanobody
   

   Use

   Tags

   Biotin acceptor peptide (Avi-tag), His14, and SUM…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TccCas13a Twin SUMO

   Plasmid

   #199754

   Purpose

   Expresses codon optimized thermostabile TccCas13a in E.coli

   Depositor

   Magdy Mahfouz

   Article

   Mahas et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

   Insert

   TccCas13a
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   LacI

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMal-T-Avi-His/BirA

   Plasmid

   #102962

   Purpose

   Vector for in vivo biotinylation of a fusion protein in bacteria.

   Depositor

   Tonia Rex

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pWZ853

   Plasmid

   #208115

   Purpose

   mSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-BSD

   Depositor

   Jef Boeke

   Article

   Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

   Insert

   mNeonGreen-HPRT1-BSD
   

   Use

   Tags

   T2A

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. luxA+luxB/pRSETB Duet

   Plasmid

   #197581

   Purpose

   Expressing luxA and luxB under dual T7 promoter

   Depositor

   Takeharu Nagai

   Article

   Kaku et al Sci Rep. 2021 Jul 22;11(1):14994. doi: 1

   Insert

   LuxA LuxB
   

   Use

   Tags

   T7 tag and 6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSAB35

   Plasmid

   #175787

   Purpose

   Expresses Legionella pneumophila SidK (1-278) with a cleavable N-terminal His tag (TEV site) and C-terminal 3xFLAG tag

   Depositor

   John Rubinstein

   Article

   Abbas et al Science. 2020 Mar 13;367(6483):1240-1246

   Insert

   SidK (1-278)
   

   Use

   Tags

   3xFLAG and 6xHis TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTNS3

    Plasmid

    #63127

    Purpose

    Tn7 transposase expression

    Depositor

    Herbert Schweizer

    Article

    Choi et al Appl Environ Microbiol. 2008 Feb;74(4):1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P1 and Ptac

    Available since

    May 17, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. T444T

    Plasmid

    #113081

    Purpose

    Enhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.

    Depositor

    Tibor Vellai

    Article

    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJH-MBP-CbAgo

    Plasmid

    #127707

    Purpose

    pET His6 MBP TEV CbAgo

    Depositor

    John van der Oost

    Article

    Hegge et al Nucleic Acids Res. 2019 May 9. pii: 5487

    Insert

    CbAgo
    

    Use

    Tags

    MPB

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLEVI(408)-ColE-iLight-msfGFP

    Plasmid

    #170268

    Purpose

    Expresses LexA_DBD-iLight-msfGFP under J23116 promoter and mCherry under ColE promoter with a LexA408 operator in bacteria.

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

    Insert

    iLight
    

    Use

    Tags

    msfGFP

    Expression

    Bacterial

    Mutation

    To design iLight for expression in bacteria, a fu…

    Promoter

    pJ23116

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBHM2403

    Plasmid

    #171687

    Purpose

    pRS316-PTEF1-CnoCas9, contains Cryptococcus neoformans codon optimized Cas9 under the control of the TEF1 promoter and terminator.

    Depositor

    Hiten Madhani

    Article

    Huang et al Genetics. 2021 Oct 23. pii: 6409193. doi

    Insert

    CnoCAS9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TEF1

    Available since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mTB3-BC2 nanobody

    Plasmid

    #210208

    Purpose

    Type 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.

    Depositor

    Brian Kay

    Article

    Grahn et al Methods Mol Biol. 2023;2702:191-203. doi

    Insert

    BC2 Nanobody
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-Sac-Abeta(M1-40)

    Plasmid

    #71876

    Purpose

    Expresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cells

    Depositor

    Dominic Walsh

    Article

    Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

    Insert

    Abeta(M1-40) (APP Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-SMAD4

    Plasmid

    #16491

    Depositor

    Bert Vogelstein

    Article

    Zawel et al Mol Cell. 1998 Mar . 1(4):611-7.

    Insert

    Smad4 (SMAD4 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

18. BirA pLEMO

    Plasmid

    #119818

    Purpose

    Express endogenous biotin ligase in E.coli

    Depositor

    Alexander Gabibov

    Article

    Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

    Insert

    Biotin ligase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Rhamnose promoter

    Available since

    April 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. KARS_pNIC-Bio3

    Plasmid

    #153051

    Purpose

    Expression construct for producing Avi tagged Lysyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    KARS (KARS1 Human)
    

    Use

    Tags

    Avi and His6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pREDusk-MCS

    Plasmid

    #188970

    Purpose

    For red light-repressed bacterial expression of gene of interest

    Depositor

    Heikki Takala

    Article

    Multamaki et al ACS Synth Biol. 2022 Aug 23. doi: 10.102

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge