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pET51b_HCAII

Plasmid

#187110

Purpose

Expression of HCAII in bacteria

Depositor

Kai Johnsson

Article

Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

Insert

HCAII

Use

Tags

Hisx10 and Strep

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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P4 cosmid

Plasmid

#196335

Purpose

P4 cosmid with CRISPR-Cas9 system

Depositor

Baojun Wang

Article

Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

Insert

genes from phage P4, CRISPR-Cas9 system

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pNMT

Plasmid

#42578

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

N-myristoyl tranferase (NMT1 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

two silent mutations T690C and T942C

Promoter

T7

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only
CD Ugi4

Plasmid

#177196

Purpose

Greengate entry vector pGGC000 modified to contain bladderwort insulator 4

Depositor

Wayne Parrott

Article

Park et al bioRxiv 2021.11.04.467280

Insert

Utricularia gibba insulator 4

Use

Tags

Expression

Bacterial and Plant

Mutation

Promoter

Available since

June 28, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pGPD2

Plasmid

#43972

Depositor

Hal Alper

Article

Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

GPD

Available since

April 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX6P1-mCherry-Nanobody

Plasmid

#70696

Purpose

Expresses GST-tagged anti-mCherry Nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

Insert

mCherry-Nanobody

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2016

Availability

Academic Institutions and Nonprofits only

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CD Ugi3

Plasmid

#177195

Purpose

Greengate entry vector pGGC000 modified to contain bladderwort insulator 3

Depositor

Wayne Parrott

Article

Park et al bioRxiv 2021.11.04.467280

Insert

Utricularia gibba insulator 3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 28, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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OTUB1

Plasmid

#25149

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

otub1.040.271 (OTUB1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

amino acid residues 40-271

Promoter

Available since

Aug. 6, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pBADZ-HisCre

Plasmid

#111187

Purpose

Helper plasmid containing Cre-recombinase gene, which is expressed in DH10MultiBac cells

Depositor

Tim Richmond

Article

Berger et al Nat Biotechnol. 2004 Dec;22(12):1583-7.

Insert

Cre recombinase

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pXTK-BB1 [Backbone_RK2-KanR_PartTypeBB]

Plasmid

#229387

Purpose

XanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_RK2-KanR_PartTypeBB and an sfGFP dropout

Depositor

Michael Springer

Article

Soltysiak et al ACS Synthetic Biology. 2025. doi: 10.102

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(3)P Probe

Plasmid

#211508

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteria

Depositor

David Murray, Hannes Maib

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

FYVE-Hrs x 2 (PIKFYVE Human)

Use

Tags

6xhis-SNAP

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV SPOP 1-374

Plasmid

#197015

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Use

Tags

6His-SUMO-TEV

Expression

Bacterial

Mutation

Promoter

Available since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-sfGFP 150TAG

Plasmid

#85493

Purpose

pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

Depositor

Ryan Mehl

Article

Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

Insert

superfolder GFP 150TAG

Use

Tags

Expression

Bacterial

Mutation

150 TAG (amino acid 150 mutated to stop codon)

Promoter

T7

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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M13KO7-ΔPS-ΔgeneIII

Plasmid

#134351

Purpose

Helper phage for M13 phage production

Depositor

Mark Isalan

Article

Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

Insert

Helper phage gene

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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MAC3-tag-N

Plasmid

#185482

Purpose

Gateway compatible destination vectors containing StrepIII/HA/UltralD

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Type

Empty backbone

Use

Tags

StrepIII-HA-UltraID

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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MAC3-tag-C

Plasmid

#185481

Purpose

Gateway compatible destination vectors containing StrepIII/HA/UltralD

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Type

Empty backbone

Use

Tags

StrepIII-HA-UltraID

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pSC101_TIMER

Plasmid

#103057

Purpose

low copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)

Depositor

Dirk Bumann

Article

Claudi et al Cell. 2014 Aug 14;158(4):722-733. doi: 1

Insert

TIMER-bac

Use

Tags

Expression

Bacterial

Mutation

mutations: S4T and S179T

Promoter

constitutive ybaJp promoter

Available since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAF-HdrB3

Plasmid

#184908

Purpose

carrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferridurans

Depositor

Radhakrishnan Mahadevan

Article

Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

Insert

Cas9-sgRNA-homology arms

Use

CRISPR; Broad-host-range

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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BirA pLEMO

Plasmid

#119818

Purpose

Express endogenous biotin ligase in E.coli

Depositor

Alexander Gabibov

Article

Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

Insert

Biotin ligase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Rhamnose promoter

Available since

April 30, 2019

Availability

Academic Institutions and Nonprofits only

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spNW30

Plasmid

#173485

Purpose

Expresses spNW30 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spNW30

Use

Tags

Histag

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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