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pOPINM

Plasmid

#26044

Depositor

Ray Owens

Publication

Berrow et al Nucleic Acids Res. 2007. 35(6):e45.

Insert

His-MBP-3C-lacZ

Tags

MAHHHHHHSSG_MBP_LEVLFQGP

Expression

Bacterial, Insect, and Mamm…

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pGS-21a his6-GST-WTPhi29DNAP

Plasmid

#166863

Purpose

To produce a dual affinity-tagged Phi29 DNA polymerase

Depositor

Paul Blainey, Stephen Quake

Publication

Blainey et al Nucleic Acids Res. 2011 Mar;39(4):e19. d

Insert

Phi29 Polymerase codon-optimized for overexpression in E. coli

Tags

GST and his6

Expression

Bacterial

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pET21a-MTF-His

Plasmid

#124133

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Methionyl-tRNA formyltransferase (fmt Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOPINE GFP nanobody:Halo:His6

Plasmid

#111090

Purpose

Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.

Depositor

Lennart Wirthmueller

Publication

Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

Insert

GFP Nanobody

Tags

Halo and His6

Expression

Bacterial, Insect, and Mamm…

Promoter

T7 promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pThermoCas9_ctrl

Plasmid

#100981

Purpose

Expresses ThermoCas9 and its sgRNA module

Depositor

John van der Oost

Publication

Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:

Inserts

Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
ThermoCas9 single guide RNA expressing module

Tags

None

Expression

Bacterial

Promoter

B. coagulans DSM 1 pta promoter and B. smithii xy…

Available since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pSupAR-MbPylRS(DiZPK)

Plasmid

#91705

Purpose

express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest

Depositor

P. Chen

Publication

Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d

Inserts

pylS
pylT

Expression

Bacterial

Mutation

L274A, C313S

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Gm-eyfp

Plasmid

#65031

Purpose

GmR mini-Tn7 vector, mobilizable, for YFP tagging bacteria

Depositor

Herbert Schweizer

Publication

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

eyfp

Expression

Bacterial

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pUC19/human IL-8

Plasmid

#17610

Depositor

Joost Oppenheim

Publication

Matsushima et al J Exp Med. 1988 Jun 1. 167(6):1883-93.

Insert

IL8 (CXCL8 Human)

Expression

Bacterial

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGEX5X.1 PINK1 WT

Plasmid

#13321

Depositor

Mark Cookson

Publication

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 kinase domain (PINK1 Human)

Tags

GST

Expression

Bacterial

Mutation

Amino acids 112-496

Available since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEX-4T1-14-3-3 zeta GST

Plasmid

#13278

Depositor

Michael Yaffe

Publication

Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Insert

14-3-3 zeta (YWHAZ Bovine)

Tags

GST

Expression

Bacterial

Available since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pRK1037

Plasmid

#8833

Depositor

David Waugh

Publication

Nallamsetty et al Protein Expr Purif. 2004 Nov . 38(1):108

Insert

TVMV protease, catalytic domain

Expression

Bacterial

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only

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p4027 GST-ubiquitin

Plasmid

#10861

Depositor

Peter Howley

Publication

Scheffner et al Cell. 1993 Nov 5. 75(3):495-505.

Insert

ubiquitin (UBI4 Budding Yeast)

Tags

GST

Expression

Bacterial

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
ER-GCaMP6f

Plasmid

#182548

Purpose

Expresses GCaMP6f on the cytosolic side of the ER (P-450site)

Depositor

Chris Richards

Publication

Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ER localized P450

Expression

Bacterial and Mammalian

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pT7II-2N4Rtau

Plasmid

#177653

Purpose

Recombinant protein synthesis of human Tau

Depositor

Jeff Kuret

Publication

Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

Insert

Tau (2N4R) (MAPT Human)

Expression

Bacterial

Promoter

T7

Available since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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spNW25

Plasmid

#173484

Purpose

Expresses spNW25 in Bl21

Depositor

Huan Bao

Publication

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spNW25

Tags

Histag

Expression

Bacterial

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pC0072 LbuCas13a His6-TwinStrep-SUMO-BsaI

Plasmid

#115267

Purpose

Expresses LbuCas13a from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Publication

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

LbuCas13a

Tags

SUMO

Expression

Bacterial

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pScaf

Plasmid

#111401

Purpose

Phagemid for producing DNA origami scaffolds, derived from pUC18 and M13mp18

Depositor

Shawn Douglas

Publication

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Type

Empty backbone

Expression

Bacterial

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pT7-7 asyn A53T

Plasmid

#105727

Purpose

Bacterial expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Publication

Rospigliosi et al J Mol Biol. 2009 May 22;388(5):1022-32.

Insert

alpha-synuclein A53T (SNCA Human)

Expression

Bacterial

Mutation

A53T

Promoter

T7

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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p2CT-His-MBP-Lba_Cas13a_WT

Plasmid

#91867

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Publication

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbaCas13a (LbaC2c2) WT

Tags

His6-MBP-TEV site

Expression

Bacterial

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pTriEx-NTOM20-mVenus-Zdk1

Plasmid

#81010

Purpose

Expression of Zdk1 anchored to mitochondria

Depositor

Klaus Hahn

Publication

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Zdk1

Tags

6x His tag, FLAG, NTOM20, and mVenus

Expression

Bacterial, Insect, and Mamm…

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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