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pSW003-PpsbA-mNeonGreen

Plasmid

#205019

Purpose

Broad host-range bacterial expression vector with constitutive PsbA promoter; mNeonGreen (codon optimized for P. fluorescens)

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

mNeonGreen

Expression

Bacterial

Mutation

mNeonGreen is codon optimized for expression in P…

Promoter

PpsbA

Available Since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR-RFP entry

Plasmid

#192278

Purpose

Entry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

RFP flanked by type V-M CRISPR repeat sequences

Use

CRISPR

Expression

Bacterial

Promoter

PJ23119

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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ER-GCaMP6f

Plasmid

#182548

Purpose

Expresses GCaMP6f on the cytosolic side of the ER (P-450site)

Depositor

Chris Richards

Article

Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

Insert

ER localized P450

Expression

Bacterial and Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBBR2k-GFPuv

Plasmid

#106383

Purpose

Anhydrotetracycline-inducible tet promoter expressing gfpuv on BBR1 origin

Depositor

Brian Pfleger

Article

Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

Insert

GFPuv

Expression

Bacterial

Promoter

tet

Available Since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pKM208

Plasmid

#13077

Depositor

Kenan Murphy

Article

Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

Insert

lacI repressor (lacI E. coli)

Expression

Bacterial

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

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pCS2(+)-zGrad

Plasmid

#119716

Purpose

Plasmid to generate zGrad mRNA to inactivate proteins in zebrafish

Depositor

Holger Knaut

Article

Yamaguchi et al Elife. 2019 Feb 8;8. pii: 43125. doi: 10

Insert

zGrad

Expression

Bacterial

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pSW003-Pc-mNeonGreen

Plasmid

#205018

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens)

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

mNeonGreen

Expression

Bacterial

Mutation

mNeonGreen is codon optimized for expression in P…

Promoter

Pc

Available Since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pET22b-csgA

Plasmid

#89581

Purpose

Express csgA curli protein with GS linker and Histaq

Depositor

Urartu Seker

Article

Abdelwahab et al Langmuir. 2017 Apr 7. doi: 10.1021/acs.l

Insert

csgA (csgA )

Tags

3xGGSG linker - TEV site - 3xGGSG linker and 6xHi…

Expression

Bacterial

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pTRK669

Plasmid

#71313

Purpose

Helper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.

Depositor

Rodolphe Barrangou, Todd Klaenhammer

Article

Russell et al Appl Environ Microbiol. 2001 Sep;67(9):4

Type

Empty backbone

Use

E. coli-gram positive bacteria shuttle vector

Expression

Bacterial

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-pBAD

Plasmid

#54762

Purpose

Localization: Protein Expression Vector , Excitation: 488, Emission: 507

Depositor

Michael Davidson

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

6xHis and EGFP

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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DHX9A

Plasmid

#42456

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

DHX9A (DHX9 Human)

Tags

His6-TEV

Expression

Bacterial

Mutation

See Comments

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pCas-MmdCas12m-CBE1

Plasmid

#192284

Purpose

MmdCas12m-CBE1 expressed under a constitutive promoter

Depositor

John van der Oost

Article

Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

Insert

MmdCas12m-CDA-UGI

Use

CRISPR

Expression

Bacterial

Mutation

D485A

Promoter

PJ23108

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pTP1183

Plasmid

#104163

Purpose

Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (1x Cysteine)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-rabbit IgG Fc specific nanobody TP897 (1xCysteine)

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pISFba1

Plasmid

#226820

Purpose

Expressing ISEre1 controled by pCas promoter, λ-red recombinase, SacB and ISFba1 guide RNA targeting pMB1 ori

Depositor

Sheng Yang

Article

Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

Insert

ISFba1

Expression

Bacterial

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pJC175e

Plasmid

#79219

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PPSP gIII, luxAB

Expression

Bacterial

Promoter

PPSP

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBS_nanorod_105b

Plasmid

#120347

Purpose

phagemid containing all components for assembly of a DNA origami nanorod (10-helix bundle)

Depositor

Hendrik Dietz

Article

Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

Insert

Staple sequences for a DNA origami nanorod

Expression

Bacterial

Promoter

none

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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3xFLAG-CUL4A-pCMV7.1

Plasmid

#155022

Purpose

N-terminal 3xFLAG-tagged CUL4A in a pCMV7.1 vector

Depositor

Andre Catic

Article

Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

Insert

Cullin 4A (CUL4A Human)

Tags

3xFLAG

Expression

Bacterial

Promoter

pCMV7.1

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJ414-PCY1

Plasmid

#98150

Purpose

Expresses PCY1 from Saponaria vaccaria possessing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1

Tags

His6-TEV

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pISEre1

Plasmid

#226819

Purpose

Expressing ISEre1 controled by pCas promoter, λ-red recombinase, SacB and ISEre1 guide RNA targeting pMB1 ori

Depositor

Sheng Yang

Article

Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

Insert

ISEre1

Expression

Bacterial

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pBK- Ma PylRS WT

Plasmid

#197573

Purpose

Expresses wild-type Methanomethylophilus alvus pyrrolysine tRNA synthetase under GlnS promoter. Used for Pyl tRNA-synthetase selections.

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Insert

M. alvus PylRS WT

Expression

Bacterial

Promoter

GlnS (constitutive)

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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