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pYTK001

Plasmid

#65108

Purpose

Part Plasmid Entry Vector to be used in the Dueber YTK system.

Depositor

John Dueber

Article

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

none

Expression

Bacterial

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-human Dynamin 1aa K44A

Plasmid

#22197

Depositor

Pietro De Camilli

Article

Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

Insert

human Dynamin 1aa K44A (DNM1 Human)

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

K44A

Available Since

Nov. 2, 2009

Availability

Academic Institutions and Nonprofits only

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pHEN2-SG4

Plasmid

#196071

Purpose

Express wild type SG4 in E.coli

Depositor

Shankar Balasubramanian

Article

Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

Insert

SG4

Tags

3XFLAG and 6XHis

Expression

Bacterial

Promoter

T7

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pIB184 (EM)

Plasmid

#90194

Purpose

E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

Depositor

Indranil Biswas

Article

Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

Type

Empty backbone

Tags

None

Expression

Bacterial

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-NanoLuc

Plasmid

#188313

Purpose

Expression of NanoLuc via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

NanoLuc

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Promoter

T7Max

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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phage UbiC scAB-GFP

Plasmid

#104998

Purpose

Lentiviral vector expressing sequence-optimized single chain Antibody-GFP fusion protein for SunTag labeling

Depositor

Jeffrey Chao

Article

Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

Insert

single chain Antibody against GCN4

Use

Lentiviral

Tags

GB1, HA tag, and sfGFP

Expression

Bacterial and Mammalian

Promoter

UbiC

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pBB540

Plasmid

#27393

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

grpE, clpB

Expression

Bacterial

Available Since

April 26, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-human Dynamin 1aa

Plasmid

#22163

Depositor

Pietro De Camilli

Article

Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

Insert

human Dynamin 1aa (DNM1 Human)

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

D744N

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oTet

Plasmid

#124663

Purpose

Expresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins via tetrameric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oTet

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET264-pUC 24xMS2V6 Loxp KANr Loxp

Plasmid

#104393

Purpose

Yeast endogenous mRNA tagging

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pET29b-dspB(E184Q)-6xHis

Plasmid

#175802

Purpose

Plasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

Insert

Dispersin B

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oTri

Plasmid

#124662

Purpose

Expresses SpyCatcher002-oTri for trimerization of SpyTag-proteins via trimeric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oTri

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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Human_TFAM_NoMTS_pET28

Plasmid

#34705

Depositor

David Chan

Article

Ngo et al Nat Struct Mol Biol. 2011 Oct 30;18(11):

Insert

TFAM (TFAM Human)

Tags

6XHis

Expression

Bacterial

Mutation

Encodes residues 43–246, corresponding to full-le…

Promoter

T7

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pET15b-His-3C-irisin

Plasmid

#122612

Purpose

Expresses in E. coli: human irisin with Nt histag and 3C protease site for cleavage

Depositor

Harold Erickson

Article

Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

Insert

irisin (ectodomain of FNDC5) (Fndc5 Bovine, Rat, Human, Mouse)

Tags

Nt: histag-3C protease site

Expression

Bacterial

Promoter

T7 polymerase

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oHex

Plasmid

#124670

Purpose

Expresses SpyCatcher002-oHex for hexamerization of SpyTag-proteins via hexameric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oHex

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pTAKN2_J23119-sgRNA

Plasmid

#108552

Purpose

Bacterial sgRNA expression vector

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Insert

2x Streptococcus pyogenes sgRNA

Expression

Bacterial

Promoter

J23119

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oPent

Plasmid

#124664

Purpose

Expresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oPent

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET21b FAST-PETase

Plasmid

#225760

Purpose

Expresses codon-optimized FAST-PETase in E. coli with a C-terminal his-tag

Depositor

Jason Boock

Article

Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

Insert

FAST-PETase

Tags

6xHis Tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Sfp pet29b C-terminal His Tag

Plasmid

#75015

Purpose

Expresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACP

Depositor

Michael Burkart

Article

Worthington et al Org Biomol Chem. 2006 Jan 7;4(1):44-6. E

Insert

Sfp

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

May 21, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-Bcatfl

Plasmid

#24193

Depositor

David Rimm

Insert

beta-catenin (CTNNB1 Human)

Tags

GST

Expression

Bacterial

Available Since

March 9, 2010

Availability

Academic Institutions and Nonprofits only