30,875 results

1. pDB007(+)

   Plasmid

   #99147

   Purpose

   Accessory plasmid for PACE of MjTyrRS variants

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   PT7 [SD8] gIII, luxAB; PproK tyrT(Opt, CUA)
   

   Expression

   Bacterial

   Promoter

   PT7 and PproK

   Available Since

   Nov. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSWAP_Entry_H2_beta_GM

   Plasmid

   #184871

   Purpose

   pSwap plasmid part with H2_beta homology arms and gentamycin cassette

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   lacZalpha,ccdB
   

   Expression

   Bacterial

   Available Since

   Jan. 3, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pET-28GG

   Plasmid

   #104655

   Purpose

   Level 2 cloning vector

   Depositor

   Marco Trujillo

   Article

   Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Feb. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. KIF3A406

   Plasmid

   #129768

   Purpose

   Expresses mouse Kinesin-2 KIF3A head and neck linker, dimerized through kinesin-1 neck-coil to 406 for biochemical assays

   Depositor

   William Hancock

   Insert

   KIF3A (Kif3a Mouse)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Truncation at 359, fused to kinesin-1 starting Al…

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. ASC-CASP1 Octamer

   Plasmid

   #164032

   Purpose

   Bacterial expression vector encoding a 5GSS-linked ASC(CARD)-CASP1(CARD) construct to express an octamer

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   CASP1, ASC (CASP1, PYCARD Human)
   

   Tags

   His6-MBP-TEV

   Expression

   Bacterial

   Mutation

   W169G (ASC), G20K (CASP1)

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpyTag003-OspC

   Plasmid

   #159998

   Purpose

   Expresses Borrelia burgdorferi OspC (20-209) C129S with N-terminal SpyTag003 fusion in bacterial cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   SpyTag003-OspC (20-209) C129S
   

   Tags

   His6-tag and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   C129S mutation improves expression level.

   Promoter

   T7

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. FtsZ

   Plasmid

   #74128

   Purpose

   Expresses FtsZ without a peptide

   Depositor

   Lynne Regan

   Insert

   FtsZ
   

   Tags

   C-terminal linker sequence (G G S G S G S S)

   Expression

   Bacterial

   Promoter

   T5-lac

   Available Since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. STK25

   Plasmid

   #39075

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   STK25 (STK25 Human)
   

   Tags

   His6-Zb-TEV

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pGST-RXRalpha

   Plasmid

   #16547

   Depositor

   Bert Vogelstein

   Article

   He et al Cell. 1999 Oct 29. 99(3):335-45.

   Insert

   RXR alpha (RXRA Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   N-terminal DNA-binding domain: aa 1-224

   Available Since

   March 24, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. ARH2-c003

    Plasmid

    #173094

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    ADPRHL1 (ADPRHL1 Human)
    

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    E2-K354, codon optimized

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKS2

    Plasmid

    #24625

    Depositor

    Jay Keasling

    Article

    Steen et al Nature. 2010 Jan 28;463(7280):559-62.

    Insert

    'tesA
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET22b_EcSSB_2

    Plasmid

    #78204

    Purpose

    Expression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensor

    Depositor

    Martin Webb

    Article

    Fili et al Nucleic Acids Res. 2010 Jul;38(13):4448-

    Insert

    ssb (W88C)
    

    Expression

    Bacterial

    Mutation

    W88C (please see depositor comment below)

    Promoter

    T7

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBSV2_2

    Plasmid

    #118226

    Purpose

    Empty E. coli - B. burgdorferi shuttle vector; kanamycin resistant

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBbE8c-RFP

    Plasmid

    #35269

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    BAD

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPR_eGFP_497

    Plasmid

    #111824

    Purpose

    Knockout eGFP

    Depositor

    Steve Shih

    Article

    Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

    Insert

    gRNA targeting eGFP 477-497
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    U6

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. HBT-GCaMP6-HA

    Plasmid

    #107433

    Purpose

    For Arabidopsis mesophyll protoplast transient expression assay

    Depositor

    Jen Sheen

    Article

    Liu et al Nature. 2017 May 18;545(7654):311-316. d

    Insert

    GCaMP6S
    

    Tags

    HA

    Expression

    Bacterial

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. TDP-43_CTD_A321G

    Plasmid

    #98670

    Purpose

    Expresses 6xHis-tagged C-terminal domain of TDP-43 with A321G

    Depositor

    Nicolas Fawzi

    Article

    Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A321G

    Available Since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEMI91

    Plasmid

    #74888

    Purpose

    Shuffled 9I27 polyprotein with shuffled codons to allow facile sequencing and unique RE for the 9 cassettes for versatile cloning. See Depositor Comments for more information

    Depositor

    Piotr Marszalek

    Article

    Scholl et al Biomacromolecules. 2016 Jun 8.

    Insert

    Titin I27 x 9 (TTN Human)
    

    Tags

    His-tag and Strep-tag II

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGT-Ah5

    Plasmid

    #122572

    Purpose

    MAGIC donor plasmid (Himar transposon with catP/sfGFP payload)

    Depositor

    Harris Wang

    Article

    Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

    Insert

    catP/sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKB01-PssbB-mKate2

    Plasmid

    #69105

    Purpose

    Expresses mKate2 (codon adapted for Streptococcus pneumoniae); under control of CSP inducible promoter PssbB. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

    Depositor

    Jan-Willem Veening

    Article

    Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

    Insert

    mKate2
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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