30,234 results

1. pKM342

   Plasmid

   #71486

   Purpose

   Contains loxP-hyg-loxP cassette for recombineering; HygR, AmpR.

   Depositor

   Kenan Murphy

   Article

   Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

   Insert

   loxP-hyg-loxP cassette
   

   Use

   Source of loxp-hyg-loxp for pcr amplification.

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. MAC-N-GFP

   Plasmid

   #172974

   Purpose

   MAC-tagged GFP expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   GFP
   

   Use

   Tags

   MAC-tag

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBSPPc

   Plasmid

   #116918

   Purpose

   Gene constitutive expression from a Pc promoter

   Depositor

   Cuiqing Ma

   Article

   Xu et al Appl Environ Microbiol. 2013 Apr;79(8):2

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pc promoter from Delftia acidovorans C17

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTH385-GST-TEV-SUP35

   Plasmid

   #29744

   Depositor

   Tobias von der Haar

   Insert

   SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   wild type

   Promoter

   Available since

   Aug. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSF-1b-S14

   Plasmid

   #128596

   Purpose

   Expresses tagless 30S ribosomal subunit protein S14 in E.coli

   Depositor

   James Williamson

   Article

   Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

   Insert

   30S ribosomal subunit protein S14 (rpsN E.coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-p40R57Q PX

   Plasmid

   #19009

   Depositor

   Michael Yaffe

   Article

   Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

   Insert

   p40phox (NCF4 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa1-148 (PX domains) site directed mutagenesis: …

   Promoter

   Available since

   Aug. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pGEX-HiGlpG

   Plasmid

   #158538

   Purpose

   Bacterial expression of rhomboid protease HiGlpG

   Depositor

   Sinisa Urban

   Article

   Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

   Insert

   HiGlpG
   

   Use

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMD18-T-pyrF

   Plasmid

   #174388

   Purpose

   modified pMD18-T containing pyrF and its native promoter, Ampr

   Depositor

   Jing Han

   Article

   Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

   Insert

   pyrF
   

   Use

   Template for cloning fragments for chromosomal in…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. PTPN4

   Plasmid

   #38828

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   PTPN4 (Ptpn4 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. Q-IL18-6

    Plasmid

    #166554

    Purpose

    scFv of a human scaffold targeting Interleukin-18. Antigen coverage aa 37-193 of 193, full-length excl. signal peptide

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, Sortag, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTK164

    Plasmid

    #13533

    Depositor

    Wolf Frommer

    Article

    Kaper et al PLoS Biol. 2007 Oct;5(10):e257.

    Insert

    FLIPW-CTY
    

    Use

    Tags

    ECFP and Venus

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYC1876

    Plasmid

    #158722

    Purpose

    Helper plasmid pYC1640 for C-terminal tagging with degradation tag ssrA

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    ssrA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKS104

    Plasmid

    #24632

    Depositor

    Jay Keasling

    Article

    Steen et al Nature. 2010 Jan 28;463(7280):559-62.

    Insert

    fadD (M3351), atfA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET42a-lambdaN+-L+-GSH

    Plasmid

    #98894

    Purpose

    Expresses lambdaN+-L+-GST fusion protein for affinity purification with ARiBo method

    Depositor

    Pascale Legault

    Article

    Di Tomasso et al Nucleic Acids Res. 2011 Feb;39(3):e18. d

    Insert

    lambdaN+-L+
    

    Use

    Tags

    GST tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Aug. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET23a-H6-TEV-cAbl

    Plasmid

    #214234

    Purpose

    Bacterial expression of the kinase domain of cAbl with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings

    Depositor

    Neel Shah

    Article

    Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1

    Insert

    ABL1 Kinase domain (ABL1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. hnRNPA2_LC_nocharge

    Plasmid

    #139118

    Purpose

    express hnRNPA2 LC with no charged residues

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    all charged residues are changed to S/Q except fo…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRE-Tn7-166

    Plasmid

    #118578

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mCardinal
    

    Use

    Minitn7 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETDuet RagA(T21N)-RagC

    Plasmid

    #99644

    Purpose

    Bacterial expression of RagA(T21N)-RagC

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    T21N

    Promoter

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQE30-roGFP1-iL

    Plasmid

    #83313

    Purpose

    Inducible expression of redox sensitive GFP in bacteria

    Depositor

    S. James Remington

    Article

    Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

    Insert

    roGFP1-iL
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    ro1(C48S/S147C/Q204C) plus X insertion, iL betwe…

    Promoter

    T5

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pIM1708-surT-PsurP-agaN-pNW33N

    Plasmid

    #44009

    Depositor

    Ichiro Matsumura

    Article

    Blanchard et al Appl Microbiol Biotechnol. 2014 May 2.

    Insert

    surT-PsurP-agaN
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Deleted EcoRI site (synonymous change, C to T at …

    Promoter

    Available since

    May 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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