30,234 results

1. pET22b-GFP-CPDSalI

   Plasmid

   #38257

   Depositor

   Aimee Shen, Matthew Bogyo

   Article

   Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

   Insert

   eGFP
   

   Use

   Tags

   6xHis and Vibrio cholerae MARTX toxin cysteine pr…

   Expression

   Bacterial

   Mutation

   cloned from pEGFPN3 (Clonetech)

   Promoter

   T7

   Available since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pGEM-HE-Glyco-Venus-bPAC(S27A)

   Plasmid

   #165490

   Purpose

   Xenopus oocyte expression of a photoactivatable adenylyl cyclase derived from bPAC using glycophorin for anchoring to the membrane with reduced dark activity

   Depositor

   Thomas Oertner

   Article

   Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

   Insert

   Glyco-Venus-bPAC(S27A)-Myc
   

   Use

   Cdna expression, xenopus oocyte

   Tags

   Myc and Venus

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYX-N-Puro-mNeon-TSC22D4

   Plasmid

   #222671

   Purpose

   endogenously tag TSC22D4 with N-terminal mNeon

   Depositor

   Jason Moffat

   Article

   Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

   Insert

   TSC22D4 (TSC22D4 Human)
   

   Use

   Tags

   mNeon

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBsVchCAST

   Plasmid

   #203812

   Purpose

   Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli

   Depositor

   Sheng Yang

   Article

   Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

   Insert

   VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAM5080

   Plasmid

   #85106

   Purpose

   Ptrc-yfp-kaiC expressed from NSII-Km vector constructed by seamless assembly. LacI expressed in opposite direction as Ptrc promoter. Functional fusion expres

   Depositor

   Susan Golden

   Article

   Cohen et al Curr Biol. 2014 Aug 18;24(16):1836-44. d

   Insert

   YFP-KaiC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. BASIC_11_RBS34-GFP-B0015

   Plasmid

   #68145

   Purpose

   storage vector for BASIC part: RBS-GFP(ORF)

   Depositor

   Geoffrey Baldwin

   Article

   Storch et al ACS Synth Biol. 2015 Apr 16.

   Insert

   RBS-34-GFP-B0015
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET23-PBD(65-150)-N-Cys

   Plasmid

   #13722

   Depositor

   Klaus Hahn

   Article

   Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

   Insert

   PBD(65-150)-N-Cys (PAK1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Met-Cys-Gly-PBD(65-150)-

   Promoter

   Available since

   March 28, 2007

   Availability

   Academic Institutions and Nonprofits only

8. MARS_pNIC-CTB10H

   Plasmid

   #153053

   Purpose

   Expression construct for producing Avi tagged Methionyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   MARS (MARS1 Human)
   

   Use

   Tags

   TEV-Avi-His10

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pETDuet-T7-Ct-WspR

   Plasmid

   #79166

   Purpose

   Dual expression of Ct biosensor and WspR WT under individual T7 promoters

   Depositor

   Ming Hammond

   Article

   Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

   Inserts

   WspR (wspR P. areuginosa)
   T7-Spinach2-Ct
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GST-AR-174-566

    Plasmid

    #104197

    Purpose

    Expresses human AR-174-566 in pGEX-3x bacterial expression vector as fusion protein with GST

    Depositor

    Elizabeth Wilson

    Article

    He et al J Biol Chem. 2000 Jul 28;275(30):22986-9

    Insert

    human androgen receptor aa 174-566 (AR Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGBW-m4136458

    Plasmid

    #149531

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHIS;Linker_GGSG;SUMO tag;Linker_GGSG;Cleavable …

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 13, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pAB094e

    Plasmid

    #79245

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PBAD rpoZ-HA4 (Y87A); PC araC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD; PC

    Available since

    May 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-6P-1-DmPABPC1_J

    Plasmid

    #146647

    Purpose

    Bacterial Expression of DmPABPC1

    Depositor

    Elisa Izaurralde

    Insert

    DmPABPC1 (pAbp Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G26S relative to NM_166265.2/NP_725750.1

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a-pro-sumo2

    Plasmid

    #25102

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    pro-SUMO2 (SUMO2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pET22b-EC12WTC9AAvi

    Plasmid

    #47925

    Purpose

    Expresses E-cadherin EC12 WT with C9A mutation and avi tag in E.Coli.

    Depositor

    Tanja Kortemme

    Article

    Ritterson et al J Am Chem Soc. 2013 Aug 7.

    Insert

    E-Cadherin EC12
    

    Use

    Tags

    6xHis, Avi Tag, and TEV Cleavage

    Expression

    Bacterial

    Mutation

    changed Cysteine 9 to Alanine

    Promoter

    T7

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. TBC1D7

    Plasmid

    #32047

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    TBC1D7 (TBC1D7 Human)
    

    Use

    Tags

    His (with TEV cleavage)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pGEX ShcD-SH2

    Plasmid

    #46488

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    ShcD (SHC4 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 512-623

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. WNV NS2B-NS3 protease (K104A - catalytically active, don't self cleave); aliases: West Nile Virus NS2B-NS3 fusion protein

    Plasmid

    #204794

    Purpose

    Bacterial expression of a fusion of West Nile NS2B-NS3 proteins

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    West Nile NS2B-NS3 fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 29, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pBad-HaloGFP-Ca0.1(Na0.1, 143-144)

    Plasmid

    #198599

    Purpose

    Green fluorescent Ca2+ ion indicator for bacterial expression.

    Depositor

    Robert Campbell

    Article

    Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

    Insert

    HaloGFP-143-144
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RP1B-PP1alpha

    Plasmid

    #51768

    Purpose

    Expresses PP1 alpha residues 7-330

    Depositor

    Wolfgang Peti

    Article

    Kelker et al J Mol Biol. 2009 Jan 9. 385(1):11-21.

    Insert

    pp1 alpha
    

    Use

    Tags

    His, TEV protease site, and Thio-6

    Expression

    Bacterial

    Mutation

    Contains PP1 alpha residues 7-330

    Promoter

    T7-lac

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only