30,875 results

1. pEC2735 (pSpy0C2)

   Plasmid

   #220279

   Purpose

   integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombination

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJUMP18-tetR_O

   Plasmid

   #127021

   Purpose

   Basic Part O- ORF; TetR; transcription factor

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Insert

   Part TetR_o
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   None

   Available Since

   July 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pE-SUMO CRYBB2

   Plasmid

   #80749

   Purpose

   Expression of human beta B2-crystallin in E. coli

   Depositor

   Larry David

   Insert

   CRYBB2 (CRYBB2 Human)
   

   Tags

   6xHIS and Smt3 SUMO

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1.H1-NP

   Plasmid

   #134367

   Purpose

   Nanoluc complementation assay. Expression of histamine receptor H1 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of the Flag epitope at N terminus of H1 receptor.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   H1-NP (HRH1 Human)
   

   Tags

   Flag and natural peptide of nanoluciferase

   Expression

   Bacterial and Mammalian

   Promoter

   T7

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBTK1007 (pSL6)

   Plasmid

   #191002

   Purpose

   Expresses red chromoprotein on a broad host range RSF1010 origin plasmid

   Depositor

   Jeffrey Barrick

   Article

   Elston et al ISME Commun. 2023 May 24;3(1):49. doi: 1

   Insert

   red chromoprotein
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   CP25

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRE-Himar-142

   Plasmid

   #185931

   Purpose

   Himar transposon delivery plasmid that delivers a green fluorescent sGFP2 protein gene

   Depositor

   Mitja Remus-Emsermann

   Article

   Stocks et al Bacteria 2022, 1(1), 56-65

   Insert

   sGFP2
   

   Expression

   Bacterial

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. DEL-pHG165a

   Plasmid

   #90064

   Purpose

   LLhG coding region on pHG165a interrupted with a single base-pair deletion, 'no repressor' control plasmid

   Depositor

   Liskin Swint-Kruse

   Article

   Meinhardt et al Nucleic Acids Res. 2012 Nov;40(21):11139

   Insert

   DEL
   

   Expression

   Bacterial

   Promoter

   Iq

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. HSPA1LA

   Plasmid

   #42429

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   HSPA1LA (HSPA1L Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   See Comments

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pET21c-eIF4A

   Plasmid

   #37230

   Depositor

   Jon Lorsch

   Article

   Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

   Insert

   TIF1 (TIF1 Budding Yeast)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

10. JMJD1CA-c089

    Plasmid

    #79644

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    JMJD1C (JMJD1C Human)
    

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-MBP-TEV-AICD

    Plasmid

    #69927

    Purpose

    bacterial expression of MBP-TEV-AICD

    Depositor

    Zita Balklava, Thomas Wassmer

    Article

    Currinn et al Cell Mol Life Sci. 2015 Jul 28.

    Insert

    AICD (APP Human)
    

    Tags

    MBP-TEV

    Expression

    Bacterial

    Mutation

    47 aa long

    Promoter

    T7

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLB052 - deAct - SpVB

    Plasmid

    #136286

    Purpose

    entry vector for GreenGate cloning method, D module, open reading frame of the mono(ADP-ribosyl) transferase domain of the Salmonella enterica SpvB

    Depositor

    Alexis Maizel

    Article

    Vilches Barro et al Curr Biol. 2019 Aug 5;29(15):2443-2454.e

    Insert

    deAct - SpvB
    

    Expression

    Bacterial

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCARSF63

    Plasmid

    #64695

    Purpose

    H6-TRX-Smt3-GB1-NpuDnaEN102-CBD

    Depositor

    Hideo Iwai

    Article

    Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

    Type

    Empty backbone

    Tags

    CBD, HisTag, NpuDnaE-N102, Smt3, and TRX

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBbE6c-RFP

    Plasmid

    #35287

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    LlacO-1

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYZ_3WJrep_N20_switch

    Plasmid

    #132722

    Purpose

    T7 RNAP-driven expression of 3WJ repressor index 20 switch RNA with a GFP-ASV reporter

    Depositor

    Alexander Green, Peng Yin

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_N20_switch
    

    Expression

    Bacterial

    Available Since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYZ_3WJrep_N20_trigger

    Plasmid

    #132726

    Purpose

    T7 RNAP-driven expression of 3WJ repressor index 20 trigger RNA

    Depositor

    Alexander Green, Peng Yin

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_N20_trigger
    

    Expression

    Bacterial

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQA-LP574

    Plasmid

    #222953

    Purpose

    Expresses R120A LoaP

    Depositor

    Adrian Ferré-D'Amaré

    Article

    Elghondakly et al Structure. 2024 Jun 28:S0969-2126(24)002

    Insert

    antiterminator protein LoaP
    

    Tags

    10xHIS-SUMO

    Expression

    Bacterial

    Mutation

    mutated Arginine 120 to Alanine

    Promoter

    T7

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-E(4130)A2

    Plasmid

    #168021

    Purpose

    Expresses E(4130)A2-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    E(4130)A2-6xHis
    

    Tags

    Hisx6

    Expression

    Bacterial

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCM291rfp

    Plasmid

    #59775

    Purpose

    pCMrfp with TrfA mutation (291E to G)

    Depositor

    Nathan Hillson

    Article

    Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

    Insert

    rfp
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. peSCBE3-NG-HF1-Hypa

    Plasmid

    #218166

    Purpose

    This plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    escbe3-NG-HF1-Hypa
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Expression

    Bacterial

    Mutation

    Mutation in the portion of deaminase hAPOBEC3A : …

    Promoter

    rpsL promoter

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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