30,880 results

1. pQTEV-YWHAE

   Plasmid

   #31562

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (YWHAE Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfuNca-Plastid

   Plasmid

   #190818

   Purpose

   Plastid targeting GFP with ble resistant gene for zeocin

   Depositor

   Peter Kroth

   Article

   Sprecher et al Algal Research. 2023 May; Vol 72: 103136

   Inserts

   Diatom plastid targeted GFP
   Bleomycin resistance gene
   

   Expression

   Bacterial

   Promoter

   Cylindrotheca fusiformis fcp and Nitzschia captiv…

   Available Since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-TRE3_ mTFP1- Vamp2

   Plasmid

   #201214

   Purpose

   AAV expression of TRE(TRE3G)-driven, Vamp2-conjugated mTFP1 expression for fluorescent labling of axon terminals

   Depositor

   Tetsuo Yamamori

   Article

   Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

   Insert

   mTFP1 (from addgene #54613)
   

   Use

   AAV

   Tags

   mouse Vamp2

   Expression

   Bacterial and Mammalian

   Promoter

   TRE3G

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTYB11-PTBP1-RRM1

   Plasmid

   #89153

   Purpose

   Recombinant expression of PTBP1-RRM1 in E.coli

   Depositor

   Frédéric Allain

   Article

   Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

   Insert

   Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 1 (PTBP1 Human)
   

   Tags

   Intein and chitin binding domain

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS-MK3

   Plasmid

   #30190

   Depositor

   Bruce Tempel

   Insert

   MK-3 (Kcna3 Mouse)
   

   Expression

   Bacterial

   Available Since

   June 3, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pBbB9a-GFP

   Plasmid

   #126112

   Purpose

   Constitutive expression vector for Escherichia coli. BglBrick compatible.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

   Insert

   GFPuv
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23113

   Available Since

   June 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGT-Ah3

   Plasmid

   #122570

   Purpose

   MAGIC donor plasmid (Himar transposon with beta-lactamase/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   beta-lactamase/sfGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJKW0957

   Plasmid

   #119026

   Purpose

   E.coli expression plasmid for PmKOMT1

   Depositor

   Jing-Ke Weng

   Article

   Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

   Insert

   PmKOMT1
   

   Tags

   8xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJBL717

   Plasmid

   #140387

   Purpose

   Expresses MobR protein under the control of T7-lacO promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-lacO-MobR-T
   

   Tags

   TEV-6XHis

   Expression

   Bacterial

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE60 R196M wgMDH

    Plasmid

    #204273

    Purpose

    Bacterial expression of R196M wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R196M

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQE60 D193E wgMDH

    Plasmid

    #204269

    Purpose

    Bacterial expression of D193E wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    D193E

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21b-LaG9(S151C)

    Plasmid

    #172490

    Purpose

    Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).

    Depositor

    Siegfried Musser

    Article

    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

    Insert

    LaG9(S151C)
    

    Tags

    6xHis, PPX cleavage site, and pelB leader sequenc…

    Expression

    Bacterial

    Mutation

    S151C

    Promoter

    T7

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBbA13c-RFP

    Plasmid

    #126098

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23102

    Available Since

    June 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. CL7-LbCas12aD156R

    Plasmid

    #164659

    Purpose

    Bacterial expression of mutated LbCas12aD156R using CL7/Im7

    Depositor

    Piyush Jain

    Article

    Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

    Insert

    LbCas12a-D156R
    

    Tags

    Thioredoxin, CL7, SUMO

    Expression

    Bacterial

    Mutation

    changed Aspartic Acid 156 to Arginine

    Promoter

    T7

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC002 - LshC2C2 locus into pACYC184

    Plasmid

    #79151

    Purpose

    Endogenous LshC2c2 locus cloned into pACYC184

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

    Insert

    LshC2c2 locus
    

    Expression

    Bacterial

    Available Since

    June 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. BHMT

    Plasmid

    #64075

    Purpose

    Expression of betaine homocysteine methyltransferase

    Depositor

    Vern Schramm

    Article

    Poulin et al J Org Chem. 2015 May 15;80(10):5344-7. d

    Insert

    betaine-homocysteine methyltransferase (BHMT Synthetic, Human)
    

    Tags

    none

    Expression

    Bacterial

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-p53

    Plasmid

    #39479

    Depositor

    Ie-Ming Shih

    Article

    Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

    Insert

    TP53 (TP53 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    P72R

    Available Since

    Sept. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pSUMO His6 SUMO AMPKa (11-281)

    Plasmid

    #177851

    Purpose

    Bacterial Expression of AMPKa1

    Depositor

    Karsten Melcher

    Article

    Li et al Cell Res. 2015 Jan;25(1):50-66. doi: 10.

    Insert

    AMPK a1 (PRKAA1 Human)
    

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLS2965

    Plasmid

    #122534

    Purpose

    Expression of a C. elegan codon optimized fluorescent protein (CemOrange2) fused to a Golgi localized gene (aman-2) under the intestinal specific promoter nhx-2

    Depositor

    Gary Silverman

    Article

    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

    Insert

    Pnhx-2_aman-2::CemOrange2 (aman-2 Nematode, Synthetic)
    

    Expression

    Bacterial

    Promoter

    Pnhx-2

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLRD04

    Plasmid

    #208258

    Purpose

    Production of ent-kaurene in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    tHMGR
    ptmT2
    blr2150
    

    Expression

    Bacterial

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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