30,239 results

1. pET28a-StrepII-TagRFP-CohI

   Plasmid

   #58710

   Purpose

   Detection construct to label dockerin type I containing fusion proteins

   Depositor

   Hermann Gaub

   Article

   Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

   Insert

   TagRFP-CohI
   

   Use

   Tags

   StrepII

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28b-ETV6-ETS

   Plasmid

   #111180

   Purpose

   Minimal ETS domain of human ETV6 (TEL)

   Depositor

   Gregory Poon

   Article

   Vo et al J Biol Chem. 2017 Aug 11;292(32):13187-1

   Insert

   ETV6 ETS domain (ETV6 Bovine, Human, Mouse)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR PKAKTRn-Clover

   Plasmid

   #177199

   Purpose

   Gateway entry vector encoding PKAKTRnew-Clover

   Depositor

   Markus Covert

   Article

   Kudo et al Cell Syst. 2022 May 18;13(5):376-387.e8.

   Insert

   PKAKTRnew-mClover
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVRc20_992

   Plasmid

   #49739

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   AS20_992
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pLux

   Available since

   Jan. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSP2262

   Plasmid

   #70703

   Purpose

   Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized Staphylococcus aureus dCas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   D10A & H557A in SaCas9

   Promoter

   T7

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBbB19a-GFP

   Plasmid

   #126131

   Purpose

   Constitutive expression vector for Escherichia coli. BglBrick compatible.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

   Insert

   GFPuv
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23105

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Human_TFAM_dHMGA_pET28

   Plasmid

   #167329

   Purpose

   Expresses TFAM dHMGA mutant in bacterial cells

   Depositor

   Tom Misteli

   Article

   Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

   Insert

   TFAM (TFAM Human)
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Truncation mutant containing linker, HMGB, and C-…

   Promoter

   T7

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. IBMc460

   Plasmid

   #161940

   Purpose

   Carries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoter

   Depositor

   Baojun Wang

   Article

   Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

   Insert

   pSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PPhlF

   Available since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBbB15a-GFP

   Plasmid

   #126130

   Purpose

   Constitutive expression vector for Escherichia coli. BglBrick compatible.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

   Insert

   GFPuv
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23100

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBbB18a-GFP

    Plasmid

    #126133

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    GFPuv
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23117

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBbB20a-GFP

    Plasmid

    #126132

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    GFPuv
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    S23200

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBbB13a-GFP

    Plasmid

    #126116

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    GFPuv
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23102

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBbB10a-GFP

    Plasmid

    #126113

    Purpose

    Constitutive expression vector for Escherichia coli. BglBrick compatible.

    Depositor

    Nigel Scrutton

    Article

    Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

    Insert

    GFPuv
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23116

    Available since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Q-ACACAA-10

    Plasmid

    #166546

    Purpose

    scFv of a human scaffold targeting Acetyl-CoA carboxylase 1. Antigen coverage aa 1572 - 2338 of 2346

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Mutation

    Promoter

    LacZ

    Available since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmCellFree_KA1_ZIKV NS2A

    Plasmid

    #145343

    Purpose

    Cell-free/mammalian expression vector for Zika virus NS2A protein with N-term eGFP

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV NS2A
    

    Use

    Tags

    8xHis and eGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7- CMV/CAG

    Available since

    Feb. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. EHF_pet28a

    Plasmid

    #131660

    Purpose

    Bacterial expression of ETS gene EHF

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    EHF (EHF Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28-OmpT

    Plasmid

    #68862

    Purpose

    construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

    Depositor

    Neil Kelleher

    Article

    Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

    Insert

    OmpT (ompT )
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pG1AK

    Plasmid

    #71736

    Purpose

    Modular shuttle vector for Geobacillus and E. coli

    Depositor

    Tom Ellis

    Article

    Reeve et al ACS Synth Biol. 2016 Jul 1.

    Type

    Empty backbone

    Use

    Synthetic Biology; Shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX-4T1-WDR5

    Plasmid

    #59969

    Purpose

    Bacterial Expression GST-WDR5

    Depositor

    Debu Chakravarti

    Article

    Kim et al Mol Cell. 2014 May 22;54(4):613-25. doi:

    Insert

    WDR5 (WDR5 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

20. gNS2B47NS3 L30SF31S

    Plasmid

    #155316

    Purpose

    DENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker L30SF31S mutant

    Depositor

    Dahai Luo

    Article

    Phoo et al Antiviral Res. 2020 Oct;182:104900. doi:

    Insert

    DENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    L30 to S, F31 to S

    Promoter

    M13

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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