33,128 results
-
Plasmid#163135PurposepLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the C-IgAP surface expression anchor, nanobody, and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsNanobody, C-IgAP autotransporterExpressionBacterialPromoterP(rhaB)Available SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only
-
6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)
Plasmid#169168PurposeConjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.DepositorInsertMAP1LC3B (MAP1LC3B Human)
Tags6X Histidine Tag, TEV cleavage site, mCherryExpressionBacterialPromoterT7 lac promoterAvailable SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
MEK1C222
Plasmid#164638PurposeBacterial expression plasmid for His6-MEK1C222DepositorInsertmitogen-activated protein kinase kinase 1 (MAP2K1 Human)
TagsHis6-tagExpressionBacterialMutationG(-19)F/S222C/C277S/C376SPromoterT7Available SinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMCSG53_Nsp15
Plasmid#167265PurposeBacterial expression of Nsp15DepositorInsertNsp15 (residues 1-346) (ORF1ab SARS-CoV-2)
TagsHis6-TEV siteExpressionBacterialMutationnoneAvailable SinceMay 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
APneg-SNAP25c
Plasmid#168013Purposenegative selection PACE Accessory PlasmidDepositorInsertSNAP25-T7PAP
ExpressionBacterialAvailable SinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
APneg-SNAP25b
Plasmid#168012Purposenegative selection PACE Accessory PlasmidDepositorInsertSNAP25-T7PAP
ExpressionBacterialAvailable SinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pID1072
Plasmid#164869PurposeThe pCYMR-1.phiKO2cB plasmid carrying the gene for phiKO2 cB repressor. Genomic integration vector for expression of any candidate DNA looping protein.DepositorInsertphiKO2 cB repressor
ExpressionBacterialAvailable SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
poRFP-oGFP
Plasmid#154130PurposeExpresses orthogonal gfp and orthogonal rfp genes in bacterial cellsDepositorInsertsorthogonal gfp
orthogonal rfp
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-MHL: nsp10_SARS-CoV-2
Plasmid#167851PurposeBacterial expression of the SARS-CoV-2 protein nsp10DepositorInsertnsp10
Tags6xHis-TEVExpressionBacterialPromoterT7Available SinceApril 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET51b-His-TEV-HOB_(HaloTag7_oligo_binder)
Plasmid#167268PurposeOverexpression of the HaloTag7 variant HOB (halo-based oligonucleotide binder) in E. coliDepositorInsertHOB
TagsHis x10ExpressionBacterialPromoterT7Available SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL1-MBP-4xDQNAT
Plasmid#128390PurposeIn vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sitesDepositorInsertMaltose binding protein
Tags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationA302T- please see depositor commentPromoterT7Available SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
ScSpe1 pET-20b(+)
Plasmid#117145PurposeExpresses the His tagged Saccharomyces cerevisiae Ornithine Decarboxylase in Escherichia coli BL21DepositorInsertSPE1
TagsHistidineExpressionBacterialPromoterT7Available SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-X(5010)B1
Plasmid#168019PurposeExpresses X(5010)B1-6xHis in bacterial cellsDepositorInsertX(5010)B1-6xHis
TagsHisx6ExpressionBacterialAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
-
pET-X(4130)B1
Plasmid#168018PurposeExpresses X(4130)B1-6xHis in bacterial cellsDepositorInsertX(4130)B1-6xHis
TagsHisx6ExpressionBacterialAvailable SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
Q-ACACAA-10
Plasmid#166546PurposescFv of a human scaffold targeting Acetyl-CoA carboxylase 1. Antigen coverage aa 1572 - 2338 of 2346DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
T-IL2-12
Plasmid#166564PurposescFv of a human scaffold targeting Interleukin-2. Antigen coverage aa 21-153 of 153, full-length excl. signal peptideDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, Sortag, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCPP5060
Plasmid#128716PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcN-hopN1
ExpressionBacterialAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
R725-X20-566: His6-MBP-tev-Avi-Hs.LGALS3
Plasmid#159700PurposeE. coli protein expressionDepositorAvailable SinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only