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pSR357.24

Plasmid

#125109

Purpose

Expression of narL(1-159aa)-ydfI(153-213aa) chimera under PLtetO-1, output Promoter PydfJ115

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL(REC)-ydfI(DBD)159
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-TEV-mDvl2DEP(416-510)_pCDFduet

Plasmid

#216387

Purpose

Bacterial expression vector for MBP-tagged mouse Dvl2 DEP domain (residues 416-510)

Depositor

William Weis

Article

Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

Insert

Dishevelled-2 (Dvl2 Mouse)

Tags

6xHis-MBP-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pTriEx-NTOM20-mVenus-Zdk3

Plasmid

#81012

Purpose

Expression of Zdk1 anchored to mitochondria

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk3

Tags

6x His tag, FLAG, NTOM20, and mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only

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LACTB2

Plasmid

#39115

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

LACTB2 (LACTB2 Human)

Tags

His Tev

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
pGEX-UAP56

Plasmid

#157661

Purpose

Expresses UAP56 in E.coli cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Spliceosome RNA helicase DDX39B (DDX39B Human)

Tags

GST tag

Expression

Bacterial

Mutation

deleted amino acids 1-43

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pDB007(+)

Plasmid

#99147

Purpose

Accessory plasmid for PACE of MjTyrRS variants

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

PT7 [SD8] gIII, luxAB; PproK tyrT(Opt, CUA)

Expression

Bacterial

Promoter

PT7 and PproK

Available Since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pET32a-hEPR

Plasmid

#199233

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Proepiregulin preproprotein (EREG Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pKB232

Plasmid

#170019

Purpose

Encodes LacI-controlled expression of surface-displayed murine CRAMP and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
CRAMP

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_TXN_Thioredoxin

Plasmid

#109778

Purpose

Protein expression and purification of TXN_Thioredoxin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

TXN_Thioredoxin (TXN Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDSG289

Plasmid

#115608

Purpose

surface-displayed Nb2, used in composability experiments; low copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPglu-600uDelta13

Plasmid

#13559

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A Delta13

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

F16A in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pDO23A

Plasmid

#27367

Depositor

Dirk Schnappinger

Article

Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

Insert

pUC/ori, bla

Use

Gateway donor vector

Expression

Bacterial

Mutation

N/A

Available Since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pKW08-Lx-Int

Plasmid

#25015

Depositor

Brian Robertson

Article

Williams et al Plasmid. 2010 Sep;64(2):69-73. doi: 10.1

Insert

pKW08-Lx-Int

Expression

Bacterial

Available Since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pET3a-Ub-K48C

Plasmid

#18897

Depositor

Cecile Pickart

Article

Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

Insert

Ubiquitin-K48C (UBC Human)

Expression

Bacterial

Mutation

Lysine 48 is replaced with a Cysteine residue

Available Since

Sept. 10, 2008

Availability

Academic Institutions and Nonprofits only
pRBBm59

Plasmid

#48115

Purpose

Shuttle vector E. coli/B.meg.

Depositor

Dieter Jahn

Article

Biedendieck et al J Biotechnol. 2007 Dec 1;132(4):426-30.

Insert

promoter sacB - gfp

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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FAK2_HUMAN_D0

Plasmid

#79747

Purpose

This plasmid encodes the kinase domain of FAK2. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

FAK2 (PTK2B Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pGEM-3XP3.RFP-tan

Plasmid

#83474

Purpose

RFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

Depositor

Patricia Wittkopp

Article

Lamb et al Fly (Austin). 2016 Aug 5:1-12.

Insert

3XP3.RFP-tan

Use

CRISPR

Expression

Bacterial and Insect

Promoter

3XP3 eye-expression Promoter

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pSB1C3 I0500 LuxI

Plasmid

#107965

Purpose

Arabinose-inducible 3OC6HSL sender plasmid

Depositor

Jim Haseloff

Article

Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

Insert

luxI

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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P48

Plasmid

#212155

Purpose

Plasmid expressing 6x His tagged secondary alcohol dehydrogenase fused with superfolder green fluorescent protein at N terminus under constitutive promoter PgyrA control.

Depositor

Kang Zhou

Insert

superfolder green fluorescent protein, secondary alcohol dehydrogenase

Tags

6x His

Expression

Bacterial

Promoter

PgyrA

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST-HemmarG2

Plasmid

#112814

Purpose

gateway cloning destination vector for expressing enhancer driven CD4-tdGFP

Depositor

Chun Han

Article

Poe et al Genetics. 2018 Nov 30. pii: genetics.118

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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