32,672 results
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Plasmid#60589Purposea 4-integrase plasmidDepositorInsertCis_2+7+8+5
ExpressionBacterialPromoterpBADAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSUMO-YHRC
Plasmid#54336PurposeExpression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.DepositorTypeEmpty backboneTags6His-SUMO (yeast gene: SMT3)ExpressionBacterialPromoterT7-LacOAvailable SinceAug. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
DTX2
Plasmid#228447PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorAvailable SinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
Human USP30 (64-502, construct 8, MG-26-21)
Plasmid#110745PurposeBacterial expression of human USP30 (construct 8)DepositorInsertUSP30 (USP30 Human)
TagsN-His6-3C-GST-3CExpressionBacterialMutationF348D, M350D, I353E + insertion deletion: 64-357;…PromoterT7Available SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)-Caprola_05
Plasmid#194666PurposeProtein production of the split HaloTag based calcium recorder Caprola_05 in bacteriaDepositorInsertCaprola_05
TagsHis-tag and Strep-tagExpressionBacterialPromoterT7Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-SpyCatcher002-MBP
Plasmid#216295PurposeExpresses SpyCatcher002 linked to maltose-binding protein in bacterial cellsDepositorInsertSpyCatcher002-MBP
TagsHis6 tag and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJCC_039 FnCas12a
Plasmid#171666PurposeFor bacterial expression of FnCas12a with a C-terminal sorting motif and His tagDepositorInsertFnCas12a
Tags6X His and sortase A sorting motifExpressionBacterialAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant
Plasmid#101217PurposeBacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variantDepositorInsertSpCas9 variant K848A/K1003A/R1060A/N497A/R661A/Q695A/Q926A
Tags10x His, MBP, and TEV siteExpressionBacterialMutationK848A, K1003A, R1060A, N497A, R661A, Q695A and Q9…PromoterT7Available SinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEDJ333
Plasmid#177272PurposeGAL1p:Cas9:CYC1tDepositorInsertCas9
ExpressionBacterial and YeastPromoterGAL1Available SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHG7
Plasmid#188255PurposeIntegrating plasmid contains the C31 int/attP locus and the hygromycin-resistance gene for selectionDepositorTypeEmpty backboneExpressionBacterialPromoteract1p/actII-orf4Available SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEB2-DsRed-Express2
Plasmid#103999PurposePlasmid encoding DsRed-Express2DepositorInsertDsRed-Express2
UseLow copyExpressionBacterialMutationMutations relative to DsRed (R2D, S4T, K5E, E10P,…PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX Stat5A-SH2
Plasmid#46515DepositorInsertStat5A (STAT5A Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 582-693PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
mCD8-GFP in pBS
Plasmid#17745DepositorAvailable SinceMay 28, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcloDF-kan
Plasmid#184837PurposeBackbone that confers Kanamycin resistance, contains cloDF oriDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-nb112
Plasmid#171702PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the spike protein of SARS-CoV-2 via a flexible linker.DepositorInsertcsgBACEFG, with nanobody fused to CsgA
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAD6
Plasmid#134349PurposeE. coli mfd gene cloned into pSKB2 with an N-terminal, ppx-cleavable His6 tagDepositorAvailable SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMU-4
Plasmid#172353PurposeCRISPR gene editing in Streptomyces, codon-optimized Cas9 behind theophylline riboswitch and D4 promoter, with custom gRNADepositorInsertD4-TheoR-cas9
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterD4Available SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pML597
Plasmid#32235PurposeExpresses M. tuberculosis codon-optimized flpe geneDepositorInsertmflpe
ExpressionBacterialMutationM.tuberculosis codon optimized flpe geneAvailable SinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBS mNFATc3-EE
Plasmid#17868DepositorAvailable SinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEM-3z/602
Plasmid#26657DepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 2, 2010AvailabilityAcademic Institutions and Nonprofits only