30,239 results
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CBX4
Plasmid#25237PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCBX4_02 (CBX4 Human)
UseTags6x His and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
4LEC
Plasmid#60091PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMETTL21A:JMC072-B07:C227766 (METTL21A Human)
UseTags6xHisExpressionBacterialMutationSee commentsPromoterT7Available sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
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pIB168
Plasmid#90191PurposeE. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pspac promoterDepositorTypeEmpty backboneUseTagsNoneExpressionBacterialMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Reb_207 aka PAS630
Plasmid#71235PurposeReb_206 with RebA(S88P)DepositorInsertReb locus
UseTagsmNeonGreen to second copy RebB, soluble mCherry a…ExpressionBacterialMutationRebA(S88P)PromoterAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-PA-Rac1-I539E
Plasmid#22026PurposeExpression of photoactivatable Rac1 ('lit' state mutant)DepositorInsertPA-Rac1-I539E (RAC1 Avena sativa (oat), Human)
UseTags6X HisExpressionBacterial, Insect, and Mamm…MutationLOV domain contains 'lit state' mutatio…PromoterCMV, p10Available sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SpSrtA
Plasmid#64987PurposeExpresses the wild type of Streptococcus pyogenes sortase A in E. coliDepositorInsertS. pyogenes sortase A
UseTagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-81PromoterT7 promoterAvailable sinceJune 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET16b-Mms2
Plasmid#18893DepositorInsertYeast ubiquitin conjugating enzyme Mms2 (MMS2 Budding Yeast)
UseTagsHIS10ExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
FGFR1_HUMAN_D0
Plasmid#79719PurposeThis plasmid encodes the kinase domain of FGFR1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertFGFR1 (FGFR1 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSET FLIPPi-260n
Plasmid#13552DepositorInsertFLIPPi WT
UseTagsECFP and EYFPExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRha-tns
Plasmid#170630PurposeExpression of CRISPR-associated transposasesDepositorInsertVchTnsABC
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMM178
Plasmid#61270PurposeAlso referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialMutationPromoterpLtetO, native, native (respectively)Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFPV25.1_dGFPmut3-AAV
Plasmid#187377PurposerpsM promoter driving the expression of destabilized GFPmut3 (containing a AAV C-terminal tail)DepositorInsertdGFPmut3_AAV
UseTagsAAVExpressionBacterialMutationC-terminal AAV tailPromoterAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-11
Plasmid#172762PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-11; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-11
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM33_N_NTD
Plasmid#156483PurposeBacterial expression of Sars-CoV2 N_NTD protein with His-tag and GST-tagDepositorInsertN_NTD (N Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEST-PLtetO-1-UTR1-TetR-deGFP-T500
Plasmid#45774DepositorInserttetR-deGFP
UseSynthetic BiologyTagsFusion protein of TetR and deGFP, with glycine-se…ExpressionBacterialMutationPromoterPLtetO-1Available sinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
SpyCas9(WT)
Plasmid#101044PurposeExpress Streptococcus pyogenes Cas9DepositorInsertSpyCas9
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_LAMA-G97 (for GFP)
Plasmid#130715PurposePlasmid encoding the bacterial expression of LAMA-G97 for GFPDepositorInsertLAMA-G97
UseTagsHisx10ExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…PromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-G-GECO1.2
Plasmid#32468DepositorInsertG-GECO1.2
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…PromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only
