30,880 results

1. pTHMT_hnRNPF_FL

   Plasmid

   #139114

   Purpose

   express FL hnRNPF with N-terminal maltose binding protein tag

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hnRNPF (HNRNPF Human)
   

   Expression

   Bacterial

   Mutation

   None

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. 4H4H

   Plasmid

   #51314

   Depositor

   Cheryl Arrowsmith

   Insert

   4H4H (EHMT1 4H4H, Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   Truncation: See Comments

   Promoter

   T7

   Available Since

   Feb. 12, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

3. pBBR1_DVK_AE(J23106-B0034m-E0040m-B0015)

   Plasmid

   #197671

   Purpose

   Expresses GFP under the control of J23106 and B0034m

   Depositor

   Eric Young

   Article

   Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

   Insert

   GFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSFDuet-1_DARPinOFF7

   Plasmid

   #182396

   Purpose

   Bacterial expression of DARPinOFF7

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   DARPinOFF7
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. ftsZ-RFP

   Plasmid

   #63842

   Purpose

   This plasmid is designed as a bacterial cell division sensor

   Depositor

   Howard Salis

   Insert

   ftsZ, mRFP1 and GFPmut3b
   

   Tags

   mRFP1

   Expression

   Bacterial

   Mutation

   Gene is translationally fused to RFP

   Available Since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. rpoS200-RFP

   Plasmid

   #63840

   Purpose

   This plasmid is designed as a bacterial stationary phase sensor

   Depositor

   Howard Salis

   Insert

   rpoS, mRFP1 and GFPmut3b
   

   Tags

   mRFP1

   Expression

   Bacterial

   Mutation

   Gene is translationally fused to RFP

   Available Since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBZCas13b-HEPN

   Plasmid

   #89901

   Purpose

   Bacterial expression for bzCas13b and crRNA with both HEPN domains mutated. New spacers can be cloned by digesting with BsaI.

   Depositor

   Feng Zhang

   Article

   Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

   Insert

   Cas13b
   

   Expression

   Bacterial

   Mutation

   R116A/H121A/R1177A/H1182A

   Promoter

   Lac

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBS ColI alpha2

   Plasmid

   #19207

   Depositor

   Cliff Tabin

   Article

   Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

   Insert

   Alpha 2 type I collagen (COL1A2 Chicken)
   

   Expression

   Bacterial

   Available Since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pJBL7025

   Plasmid

   #128810

   Purpose

   Fluoride riboswitch-regulated catechol 2,3-dioxygenase

   Depositor

   Julius Lucks

   Article

   Thavarajah et al ACS Synth Biol. 2019 Dec 12. doi: 10.102

   Insert

   Fluoride riboswitch-regulated catechol 2,3-dioxygenase
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSW003

    Plasmid

    #111249

    Purpose

    Broad host-range bacterial vector

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    July 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGBK CUP1 Empty

    Plasmid

    #169710

    Purpose

    Yeast two-hybrid vector using a copper inducible CUP1 promoter

    Depositor

    Bruce Hamilton

    Article

    Hernandez et al bioRxiv

    Type

    Empty backbone

    Tags

    GAL4 DNA Binding Domain and Myc

    Expression

    Bacterial and Yeast

    Promoter

    CUP1

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKB230

    Plasmid

    #170017

    Purpose

    Encodes LacI-controlled expression of surface-displayed protegrin-1 and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    Protegrin-1
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Promoter

    Ptac and PvirK

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJUMP28-1A(sfGFP)

    Plasmid

    #126976

    Purpose

    Level 1 vector. sfGFP reporter. Origin pUC (high copy number)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28b-miniR-DBCO

    Plasmid

    #181984

    Purpose

    Used to label proteins with the MiniE genetically encoded tag

    Depositor

    Kimberly Beatty

    Article

    Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

    Insert

    MiniR-DBCO
    

    Tags

    DBCO-Tag, His-Tag

    Expression

    Bacterial

    Available Since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDZ617 pKAN 12xPP7 V4

    Plasmid

    #72237

    Purpose

    Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

    Depositor

    Daniel Zenklusen

    Article

    Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

    Insert

    12xPP7 V4
    

    Use

    Cre/Lox

    Expression

    Bacterial

    Available Since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMAK462

    Plasmid

    #140700

    Purpose

    NBαMs/Rt-IgG1-Fc

    Depositor

    Takayuki Iwaki

    Article

    Iwaki et al Protein Expr Purif. 2020 Feb 13;170:1056

    Insert

    NBαMs/Rt-IgG1-Fc
    

    Expression

    Bacterial

    Available Since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHS1182

    Plasmid

    #205964

    Purpose

    NZ_CP025815 DinG HNH crRNA expression in pCOLADuet-1

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    DinG HNH crRNA
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHS1543

    Plasmid

    #205963

    Purpose

    NZ_CP025815 DinG HNH Cascade proteins operon (E. coli codon optimized) with His14-tagged Csf3 in pET45b(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    DinG HNH Cascade with His14-tagged Csf3
    

    Tags

    14xHis on Csf3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pE3c

    Plasmid

    #17463

    Purpose

    modified pENTR vector for C-terminal 6x MYC tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    6xMyc

    Expression

    Bacterial, Insect, Mammalia…

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCR-BluntII-TOPO-RhoC

    Plasmid

    #14099

    Depositor

    Richard Hynes

    Article

    Clark et al Nature. 2000 Aug 3. 406(6795):532-5.

    Insert

    Human RhoC (RHOC Human)
    

    Expression

    Bacterial

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only