30,249 results

1. pANTO5

   Plasmid

   #131106

   Purpose

   To generate an integrative plasmid carrying the TetR/Pip-OFF system, but not the integrase

   Depositor

   Riccardo Manganelli

   Article

   Boldrin et al Sci Rep. 2019 Apr 8;9(1):5783. doi: 10.1

   Insert

   tetR gene by pFRA61 (Boldrin et al 2010)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM6-RgTALsyn-HpaBC

   Plasmid

   #100949

   Purpose

   Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

   Depositor

   Mattheos Koffas

   Article

   Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

   Inserts

   RgTALsyn
   HpaB
   HpaC
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Silent mutation to remove internal NdeI site on T…

   Promoter

   T7

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-PLS-KP6_rpoA-U

   Plasmid

   #190967

   Purpose

   Test the U-to-C editing activity of KP6 on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP6
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET23a-HsUBB

   Plasmid

   #69562

   Purpose

   Bacterial expression; in vitro transcription/translation (T7)

   Depositor

   Jorge Eduardo Azevedo

   Article

   Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

   Insert

   UBB (UBB Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pMD19T-psba1-TetR-PL22-dCas9-SpR

   Plasmid

   #73223

   Purpose

   Contains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.

   Depositor

   Paul Hudson

   Article

   Yao et al ACS Synth Biol. 2015 Dec 28.

   Insert

   dCas9 from S. pyogenes
   

   Use

   Tags

   c-myc

   Expression

   Bacterial

   Mutation

   Silent mutation at bp 1341 A->C to remove an E…

   Promoter

   PL22

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET30-2-PSPH

   Plasmid

   #83909

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   phosphoserine phosphatase (PSPH Human)
   

   Use

   Tags

   6x His tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. L1-E309K

   Plasmid

   #15487

   Depositor

   Vance Lemmon

   Article

   Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

   Insert

   L1-E309K (L1CAM Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Glutamic acid 309 changed to Lysine

   Promoter

   Available since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pHH0103 NEDD4 WW domain #3

   Plasmid

   #104210

   Purpose

   Bacterial expression of WW domain #3 from NEDD4

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   NEDD4 WW domain #3 (NEDD4 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3.5VPIII.EGFP.IGR2.1

   Plasmid

   #40865

   Depositor

   Harold Gainer

   Article

   Fields et al J Neurosci. 2003 Aug 27;23(21):7801-9.

   Insert

   Mouse vasopressin gene (Avp Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   AVP

   Available since

   Nov. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJKR-L-mphR

    Plasmid

    #62560

    Purpose

    Macrolide biosensor. Low copy plasmid that expresses GFP proportional to macrolide concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. ZP10 Snail

    Plasmid

    #16931

    Depositor

    Randall Moon

    Insert

    snail (snai1a Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

12. pGBW-m4046733

    Plasmid

    #145665

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 27, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pHH0103_GNA15_G-alpha

    Plasmid

    #109782

    Purpose

    Protein expression and purification of GNA15_G-alpha

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    GNA15_G-alpha (GNA15 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-45b-RUNX2(WT)-IDR-mCherry

    Plasmid

    #145286

    Purpose

    Bacterial Expression of 6xHis RUNX2(WT) IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    RUNX2(WT)-IDR (RUNX2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRU1100

    Plasmid

    #14465

    Depositor

    Philip Poole

    Article

    Karunakaran et al Microbiology. 2005 Oct . 151(Pt 10):3249

    Insert

    unstable gfp-mut3.1 LVA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSb_init containing the TM287/288 nanobody Nb_TM#2

    Plasmid

    #132698

    Purpose

    expresses the TM287/288 nanobody Nb_TM#2 in E.coli, to use as positive control in ELISA

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    nanobody Nb_TM#2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CelTag Plasmid

    Plasmid

    #66562

    Purpose

    Encodes CelTag for the tagging and high affinity purification of endogenous yeast proteins.

    Depositor

    David Engelke

    Article

    Carrick et al G3 (Bethesda). 2015 Dec 29. pii: g3.115.

    Insert

    CelTag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFA6a-mMaple3-kanMX6

    Plasmid

    #105165

    Purpose

    Yeast gene targeting

    Depositor

    Jian-Qiu Wu

    Article

    Liu et al Mol Biol Cell. 2016 Aug 15;27(16):2528-4

    Insert

    mMaple3-kanMX6
    

    Use

    Tags

    GlySerGly-mMaple3

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pScaf-10080.1

    Plasmid

    #111410

    Purpose

    pScaf phagemid with insert for generating DNA origami scaffold 10080 bases in length

    Depositor

    Shawn Douglas

    Article

    Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

    Insert

    pScaf-ACKN
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SPI1_pet28a

    Plasmid

    #131653

    Purpose

    Bacterial expression of ETS gene SPI1

    Depositor

    Peter Hollenhorst

    Article

    Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

    Insert

    SPI1 (SPI1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge