30,410 results

1. DP6

   Plasmid

   #140446

   Purpose

   Untargetted bacterial drift

   Depositor

   David Liu

   Article

   Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

   Insert

   dnaQ926 dam seqA emrR ugi CDA1 gIII
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1GFP/RNase H1 D210N

   Plasmid

   #174448

   Purpose

   Express GFP-tagged RNase H1 D210N in E. coli.

   Depositor

   Karlene Cimprich

   Article

   Crossley et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

   Insert

   RNase H1 (RNASEH1 Human)
   

   Use

   Tags

   His-tag, GFP

   Expression

   Bacterial

   Mutation

   D210N

   Promoter

   T7 promoter

   Available Since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET_RTX(exo-)-6xHis

   Plasmid

   #145028

   Purpose

   pET vector for IPTG-inducible, T7-based expression of the exonuclease deficient version of RTX bearing a C-terminal 6xHis tag

   Depositor

   Andrew Ellington

   Article

   Bhadra et al BioProtocol 2021

   Insert

   RTX(exo-)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N210D

   Promoter

   T7

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBZS-M.CviQIX (NDel15)

   Plasmid

   #198357

   Purpose

   Express M.CviQIX (NDel15) in bacterial cells.

   Depositor

   Takashi Ito

   Article

   Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

   Insert

   M.CviQIX
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-6xHis-SUMO-MarathonRT

   Plasmid

   #109029

   Purpose

   Group II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”

   Depositor

   Anna Pyle

   Article

   Zhao et al RNA. 2018 Feb;24(2):183-195. doi: 10.126

   Insert

   ERE_01490
   

   Use

   Tags

   6xHis-SUMO tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-pBAD

   Plasmid

   #54630

   Purpose

   Localization: Protein Expression Vector , Excitation: 587, Emission: 610

   Depositor

   Nathan Shaner, Roger Tsien, Michael Davidson

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Type

   Empty backbone

   Use

   Tags

   6xHis and mCherry

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSET.HaloTag.v7

   Plasmid

   #226197

   Purpose

   bacterial expression plasmid for HaloTag v7, N-terminus 6xHIS tag for affinity purification

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Insert

   HaloTag.v7
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AARS_C2_pNIC-Bio3

   Plasmid

   #153047

   Purpose

   Expression construct for producing Avi tagged Alanyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   AARS (AARS Human)
   

   Use

   Tags

   Avi and His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. PARP1 FL

   Plasmid

   #169815

   Purpose

   Expresses codon optimised full length His tagged PARP1 in bacterial cells

   Depositor

   David Neuhaus

   Article

   Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

   Insert

   Poly[ADP-ribose] polymerase 1 (PARP1 Synthetic, Human, Codon optimised)
   

   Use

   Tags

   MKHHHHHHMKQ

   Expression

   Bacterial

   Mutation

   Valine 762 mutated to an alanine

   Promoter

   T7 promoter

   Available Since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTriExRhoA2G

    Plasmid

    #40176

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    RhoA Biosensor
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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