30,258 results

1. pET29a-YS14

   Plasmid

   #66890

   Purpose

   Polycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)

   Depositor

   Jung-Hyun Min

   Article

   Shim et al Anal Biochem. 2012 Aug 15;427(2):190-2.

   Inserts

   His6-Thrombin-Xenopus laevis histones H2A
   Xenopus laevis histones H2B
   Xenopus laevis histones H3
   Xenopus laevis histones H4-Thrombin
   

   Use

   Tags

   H2A, H2B, H3, H4, His6 tag, and Thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMS31: pTwinStrep-SUMO-PmcTnsAB

   Plasmid

   #168164

   Purpose

   PmcTnsAB expression construct for protein purification.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Insert

   PmcCAST TnsAB
   

   Use

   Tags

   6X His, Twin Strep and SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. BirA His-tag pLEMO

   Plasmid

   #119817

   Purpose

   Express endogenous biotin ligase in E.coli

   Depositor

   Alexander Gabibov

   Article

   Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

   Insert

   Biotin ligase
   

   Use

   Tags

   6-Histidine-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pInt

   Plasmid

   #18941

   Depositor

   Michele Calos

   Article

   Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

   Insert

   phiC31 integrase (int Streptomyces phage phiC31)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   under lacZ promoter

   Promoter

   Available since

   Aug. 13, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCas9-NT

   Plasmid

   #235165

   Purpose

   Expresses the codon harmonized Streptococcus pyogenes cas9 gene and a NT sgRNA

   Depositor

   Constantinos Patinios

   Article

   Della Valle et al bioRxiv 2024.11.24.625072; doi: 10.1101/

   Insert

   SpCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEF1-Pls1-sfGFP

   Plasmid

   #205748

   Purpose

   Expresses sfGFP under IPTG inducible promoter Pls1 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. Low strength.

   Depositor

   Jerome Bonnet

   Article

   Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95

   Type

   Empty backbone

   Use

   Shuttle vector gram+ gram-

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pls1 (Phyperspank mutant, IPTG inducible)

   Available since

   Aug. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCas12a-NT

   Plasmid

   #235167

   Purpose

   Expresses the Wild Type Francisella novicida cas12a gene and a NT crRNA

   Depositor

   Constantinos Patinios

   Article

   Della Valle et al bioRxiv 2024.11.24.625072; doi: 10.1101/

   Insert

   FnCas12a
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSIBR2.0-818-Cas12a-NT

   Plasmid

   #235168

   Purpose

   Expresses the SIBR2.0-818-Cas12a system and a NT crRNA

   Depositor

   Constantinos Patinios

   Article

   Della Valle et al bioRxiv 2024.11.24.625072; doi: 10.1101/

   Insert

   SIBR2.0-818-Cas12a
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p300 KAT LIC 1M

   Plasmid

   #233587

   Purpose

   Bacterial expression of p300 KAT enzyme

   Depositor

   Scott Showalter

   Article

   Dewing et al Proteins. 2025 Jun 1. doi: 10.1002/prot.

   Insert

   p300 KAT (EP300 Human)
   

   Use

   Tags

   6xHis MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PrecA-CFP--PrecA-YFP

    Plasmid

    #228097

    Purpose

    recA promoter driving expression of CFP reporter and recA promoter driving expression of YFP reporter

    Depositor

    Mary Dunlop

    Article

    Alnahhas et al mSystems. 2025 May 20:e0158824. doi: 10.

    Insert

    CFP & YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    recA & recA

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pQE-80L 2xHis-Rab29

    Plasmid

    #186021

    Purpose

    2xHis-Rab29

    Depositor

    Suzanne Pfeffer

    Article

    Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

    Insert

    2xHis-Rab29 (RAB29 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBBR1_DVK_AE(J23106-RiboJ-UTR1-E0040m-B0015)

    Plasmid

    #197674

    Purpose

    Expresses GFP under the control of J23106 and RiboJ-UTR1

    Depositor

    Eric Young

    Article

    Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBBR1_DVK_AE(J23100-RiboJ-BCD2-E0040m-B0015)

    Plasmid

    #197661

    Purpose

    Expresses GFP under the control of J23100 and RiboJ-BCD2

    Depositor

    Eric Young

    Article

    Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBBR1_DVK_AE(J23103-RiboJ-BCD2-E0040m-B0015)

    Plasmid

    #197667

    Purpose

    Expresses GFP under the control of J23103 and RiboJ-BCD2

    Depositor

    Eric Young

    Article

    Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. His SUMO HNRNPA1 dHexa

    Plasmid

    #197013

    Purpose

    E. coli expression vector

    Depositor

    Tanja Mittag

    Article

    Molliex et al Cell. 2015 Sep 24;163(1):123-33. doi: 10

    Insert

    HNRNPA1
    

    Use

    Tags

    6His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBbdCas9S

    Plasmid

    #149638

    Purpose

    parental, all-in-one CRISPRi vector for B. burgdorferi, empty

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pOPINF

    Plasmid

    #26042

    Depositor

    Ray Owens

    Article

    Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

    Insert

    His3C-lacZ
    

    Use

    Tags

    MAHHHHHHSSGLEVL

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    May 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKDsgRNA-p15

    Plasmid

    #62656

    Purpose

    Arabinose inducible lambda red and anhydrotetracycline inducible sgRNA expression that targets pCas9 plasmid

    Depositor

    Kristala Prather

    Article

    Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

    Insert

    exo, beta, gam, sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a_T7-ARG1

    Plasmid

    #106473

    Purpose

    Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli

    Depositor

    Mikhail Shapiro

    Article

    Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

    Insert

    ARG1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMBP-AsCas12a

    Plasmid

    #113430

    Purpose

    Expresses 10xHis-MBP fused to AsCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    AsCas12a
    

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge