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pGEX-RIP

Plasmid

#11972

Depositor

Jon Ashwell

Publication

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

RIP (Ripk1 Mouse)

Tags

GST

Expression

Bacterial

Available since

July 13, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
k3(long) luciferase

Plasmid

#11110

Depositor

Anjana Rao

Publication

Tsai et al Mol Cell Biol. 1996 Feb . 16(2):459-67.

Insert

k3(L) (TNF Human)

Tags

luciferase

Expression

Bacterial

Available since

April 24, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
Human LFA-1 alpha

Plasmid

#8630

Depositor

Timothy Springer

Publication

Larson et al J Cell Biol 1989 Feb;108(2):703-12.

Insert

LFA1 alpha (ITGAL Human)

Expression

Bacterial

Available since

June 23, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pKL2299A

Plasmid

#222105

Purpose

A ternary vir helper plasmid that carries Agrobacterium tumefaciens virulence genes for enhanced plant genetic transformation.

Depositor

Kan Wang

Publication

Aliu et al Front Plant Sci. 2024 Jul 23;15:1429353.

Insert

virA

Expression

Bacterial and Plant

Promoter

virA promoter

Available since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b-RvLEAMshort

Plasmid

#218122

Purpose

Expresses truncated RvLEAM protein under IPTG induction in bacterial host cells.

Depositor

Ci Ji Lim

Publication

Abe et al bioRxiv [Preprint]. 2024 Feb 11:2024.02.

Insert

RvLEAMshort

Tags

6xHIS

Expression

Bacterial

Mutation

Truncated form (58-181aa)

Promoter

T7

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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PARP1 FL

Plasmid

#169815

Purpose

Expresses codon optimised full length His tagged PARP1 in bacterial cells

Depositor

David Neuhaus

Publication

Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

Insert

Poly[ADP-ribose] polymerase 1 (PARP1 Codon optimised, Synthetic, Human)

Tags

MKHHHHHHMKQ

Expression

Bacterial

Mutation

Valine 762 mutated to an alanine

Promoter

T7 promoter

Available since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET51b-His-TEV-HaloTag7

Plasmid

#167266

Purpose

Overexpression of HaloTag7 in E. coli

Depositor

Kai Johnsson

Publication

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

HaloTag7

Tags

His x10

Expression

Bacterial

Promoter

T7

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pOPINO_NbH11-H4

Plasmid

#155364

Purpose

Expresses nanobody H11-H4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2

Depositor

Ray Owens

Publication

Huo et al Nat Struct Mol Biol. 2020 Jul 13. pii: 1

Insert

H11-H4

Tags

His6

Expression

Bacterial

Promoter

T7

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pTOX6

Plasmid

#127451

Purpose

Encodes Tse2 toxin and amilCP chromoprotein. CAM-R

Depositor

Matthew Waldor

Publication

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

tse2 toxin
amilCP chromoprotein

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

pRha and tac

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pQE30-His-AlaRS

Plasmid

#124103

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Alanyl-tRNA ligase (alaS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET15b-His-RF2

Plasmid

#124130

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Release factor 2 (prfB Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21a-GluRS-His

Plasmid

#124109

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Glutamyl-tRNA ligase (gltX Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pD454-SR mouse Alpha-synuclein

Plasmid

#89075

Purpose

bacterial expression of mouse Alpha-synuclein

Depositor

Michael J Fox Foundation MJFF

Insert

Alpha-synuclein (Snca Mouse)

Expression

Bacterial

Promoter

T7

Available since

March 17, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pGEX-4T-3-mR7BD

Plasmid

#79149

Purpose

bacterial expression of GST-mR7BD (Rab7 binding domain of murine RILP)

Depositor

Aimee Edinger

Publication

Romero Rosales et al Mol Biol Cell. 2009 Jun;20(12):2831-40.

Insert

R7BD (Rilp Mouse)

Tags

GST

Expression

Bacterial

Mutation

amino acids 220-299

Promoter

tac

Available since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pUC18T-mini-Tn7T-Gm-ecfp

Plasmid

#65030

Purpose

GmR mini-Tn7 vector, mobilizable, for CFP tagging bacteria

Depositor

Herbert Schweizer

Publication

Choi et al Nat Protoc. 2006;1(1):153-61.

Insert

ecfp

Expression

Bacterial

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET-Trx-mSA2

Plasmid

#52320

Purpose

Solubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)

Depositor

Sheldon Park

Publication

Demonte et al Appl Microbiol Biotechnol. 2014;98(14):6

Insert

monomeric streptavidin 2

Tags

FLAG, His, S tag, TEV site, and Thioredoxin

Expression

Bacterial

Mutation

S25H mutation compared to streptavidin

Promoter

T7

Available since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBAD/HisB-iRFP720

Plasmid

#45460

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Expression

Bacterial

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

araBAD

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Pet28a_Human_H3.3

Plasmid

#42632

Depositor

Joe Landry

Insert

Human H3.3 (H3-3A Human)

Tags

Untagged

Expression

Bacterial

Promoter

T7

Available since

Feb. 25, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

4 Articles

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pExodus CMV.Trex2

Plasmid

#40210

Purpose

Mammalian expression of codon-optimized Trex2.

Depositor

Andrew Scharenberg

Publication

Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Three prime repair exonuclease 2 (Trex2 Mouse)

Expression

Bacterial and Mammalian

Promoter

CMV and T7

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pEGFP-C3-ZO-2

Plasmid

#27422

Depositor

Marius Sudol

Publication

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Tags

GFP

Expression

Bacterial and Mammalian

Available since

April 15, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles