30,880 results

1. lacI-Ptac-gusA-pBAV1k

   Plasmid

   #30503

   Depositor

   Ichiro Matsumura

   Article

   Murin et al Appl Environ Microbiol. 2011 Oct 21.

   Insert

   beta-glucuronidase (gusA Escherichia coli)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Nov. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTC-mcs

   Plasmid

   #20317

   Depositor

   Dirk Schnappinger

   Article

   Klotzsche et al Nucleic Acids Res. 2009 Apr;37(6):1778-8

   Insert

   multiple cloning site
   

   Expression

   Bacterial

   Available Since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBS Wnt-3aHA

   Plasmid

   #17374

   Depositor

   Jan Kitajewski

   Article

   Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

   Insert

   Wnt-3aHA (Wnt3a Mouse)
   

   Tags

   HA

   Expression

   Bacterial

   Available Since

   July 7, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pMal-E4rGel

   Plasmid

   #51280

   Purpose

   Expresses a fibronectin fusion with gelonin targeting EGFR in bacteria

   Depositor

   Dane Wittrup

   Article

   Pirie et al J Biol Chem. 2011 Feb 11;286(6):4165-72.

   Insert

   E4rGel
   

   Expression

   Bacterial

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLQ-KO-tgt50

   Plasmid

   #126578

   Purpose

   CRISPR-Cas9 based efficient genome editing in S. aureus

   Depositor

   Sheng Yang

   Article

   Liu et al Acta Biochim Biophys Sin (Shanghai). 201

   Insert

   Cas9-Pxyltet-sgRNA-Pspac-donor-tgt50
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET30-2-PSPH

   Plasmid

   #83909

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   phosphoserine phosphatase (PSPH Human)
   

   Tags

   6x His tags

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. TAF1

   Plasmid

   #39118

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   TAF1 (TAF1 Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

8. SRpHi3

   Plasmid

   #102543

   Purpose

   Bacterial expression of sepHluorin fused to the cell penetrating peptide TAT with a GST tag for purification

   Depositor

   Stefan Hell

   Article

   Richardson et al Nat Commun. 2017 Sep 18;8(1):577. doi: 1

   Insert

   Tat-sepHluorin
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTSSX-STC

   Plasmid

   #115668

   Purpose

   E.coli expression plasmid of small tetraheme cytochrome (STC) from Shewanella oneidensis MR-1 with cleavable Strep tag

   Depositor

   David Kramer

   Article

   Huang et al J Am Chem Soc. 2020 Jun 10;142(23):10459

   Insert

   small tetraheme cytochrome c
   

   Tags

   Signal peptide (aa1-27)-Strep-II tag- Factor Xa c…

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJM160

    Plasmid

    #134169

    Purpose

    Bacterial expression of RB3 stathmin-like domain (SLD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    RB3-SLD (STMN4 Human)
    

    Expression

    Bacterial

    Mutation

    residues 49-189

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBXC3GH

    Plasmid

    #47070

    Purpose

    FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Tags

    3C protease site (PreScission site), GFP, and dec…

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSB1C3 I0500 LasI

    Plasmid

    #107966

    Purpose

    Arabinose-inducible 3OC12HSL sender plasmid

    Depositor

    Jim Haseloff

    Article

    Boehm et al Nat Commun. 2018 Feb 22;9(1):776. doi: 1

    Insert

    lasI
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Q-ACACAA-10

    Plasmid

    #166546

    Purpose

    scFv of a human scaffold targeting Acetyl-CoA carboxylase 1. Antigen coverage aa 1572 - 2338 of 2346

    Depositor

    Susanne Gräslund

    Insert

    single chain-Fv, scFv
    

    Use

    Recombinant antibody expression

    Tags

    3xFLAG, His6 and OmpA leader sequence

    Expression

    Bacterial

    Promoter

    LacZ

    Available Since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. R989-X28-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D

    Plasmid

    #159548

    Purpose

    E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Insert

    His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D (KRAS Human)
    

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    G12D

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAM005

    Plasmid

    #170009

    Purpose

    Encodes PvirK-sfgfp reporter and TetR-controlled expression of S. Typhimurium PhoP

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    Superfolder GFP
    PhoP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PLTetO-1 and PvirK

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET151-D-TOPO-PARP14-SΔK

    Plasmid

    #216658

    Purpose

    Expresses multidomain construct of PARP14 with internal SUMO domain (PARP14-SΔK) in bacterial cells.

    Depositor

    Herwig Schüler

    Article

    Chatzicharalampous et al Protein Expr Purif. 2024 Aug 16:106580.

    Insert

    PARP14-SΔK DNA sequence (PARP14 G784-K1808 with internal SUMO-TEV sequence) (PARP14 Human, Synthetic)
    

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    Deletion starting from Q1390 to D1448 and substit…

    Promoter

    T7

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC57_beta-globin-5'UTR_Nanoluc

    Plasmid

    #219989

    Purpose

    betaglobin-5'UTR-NanoLuc mRNA synthesis

    Depositor

    Simon Alberti

    Insert

    betaglobin-5'UTR-NanoLuc (HBB Human)
    

    Expression

    Bacterial

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETTEV-SENP8

    Plasmid

    #196361

    Purpose

    protein expression of the His-tagged full length protein

    Depositor

    Stefan Knapp

    Insert

    SENP8/NEDP1
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRU1100

    Plasmid

    #14465

    Depositor

    Philip Poole

    Article

    Karunakaran et al Microbiology. 2005 Oct . 151(Pt 10):3249

    Insert

    unstable gfp-mut3.1 LVA
    

    Expression

    Bacterial

    Available Since

    April 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJKW0776

    Plasmid

    #119024

    Purpose

    E.coli expression plasmid for PmSPS2

    Depositor

    Jing-Ke Weng

    Article

    Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

    Insert

    PmSPS2
    

    Tags

    8xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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