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pMG81-MBP-t-M.MpeI-N374K-His

Plasmid

#197985

Purpose

Expresses CpG Carboxymethyltransferase (M.MpeI N374K) in bacteria. The gene also encodes an N-terminal (TEV cleavable) MBP tag as well as C-terminal His tag.

Depositor

Rahul Kohli

Article

Wang et al Nat Chem Biol. 2023 Jun 15. doi: 10.1038

Insert

MBP-t-M.MpeI-N374K-His

Tags

His and MBP

Expression

Bacterial

Mutation

M.MpeI-N374K

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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ARH2-c003

Plasmid

#173094

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

ADPRHL1 (ADPRHL1 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

E2-K354, codon optimized

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pRU1098

Plasmid

#14463

Depositor

Philip Poole

Article

Karunakaran et al Microbiology. 2005 Oct . 151(Pt 10):3249

Insert

unstable gfp-mut3.1 LAA

Expression

Bacterial

Available Since

April 23, 2007

Availability

Academic Institutions and Nonprofits only

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pGEX Stat5B-SH2

Plasmid

#46516

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat5B (STAT5B Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 593-692

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5DL491-492

Plasmid

#198290

Purpose

Produces PVCs retargeted with non-binding mutant of Ad5 knob

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-Ad5DL491-492

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1 U1-C-FL

Plasmid

#129077

Purpose

generation of GST-tagged U1-C protein using bacteria

Depositor

Takeshi Tsubata

Insert

U1-C (SNRPC Human)

Expression

Bacterial

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-TGFα-GSY-SPM

Plasmid

#163615

Purpose

Expresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.

Depositor

Mark Howarth

Article

Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

Insert

TGFa-GSY-SPM (TGFA Human, Synthetic)

Tags

C-tag and His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pMYO127TAG-SepT

Plasmid

#34625

Depositor

Jesse Rinehart, Dieter Söll

Article

Park et al Science. 2011 Aug 26;333(6046):1151-4.

Insert

Myoglobin-127TAG

Tags

6xHIS

Expression

Bacterial

Mutation

codon-optimized + an amber stop codon was introdu…

Promoter

Ipp

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pHis2-Arf1 (17-181)-Q71L

Plasmid

#184808

Purpose

Bacterial expression of Arf1 (aa17-181, Q71L mutation)

Depositor

James Hurley

Article

Ren et al Cell. 2013 Feb 14;152(4):755-67. doi: 10

Insert

Arf1 (ARF1 Human)

Tags

His6

Expression

Bacterial

Mutation

aa 17-181 only, Q71L

Promoter

T7

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pDJSVT91

Plasmid

#109305

Purpose

Express periplasmic, outer membrane, or secreted proteins in E. coli - SufI signal (TAT) and His6 tag

Depositor

Daniel Slade

Article

Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

Type

Empty backbone

Tags

SufI 1-30, 6His

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pQTEV-FLJ30046

Plasmid

#34828

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

FLJ30046 (SLAIN1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pET15b-sfGO-Matryoshka-LE-LP

Plasmid

#100018

Purpose

Bacterial expression of fluorescent protein casette - cpsfGFP with nested LSSmOrange and LE N-term and LP C-term amino acid linkers

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

cpsfGFP-LSSmOrange

Tags

6x HIS tag

Expression

Bacterial

Mutation

Leu and Glu N-term linker and Leu and Pro C-term …

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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NR4A1

Plasmid

#25477

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

NR4A1 (NR4A1 Human)

Tags

His

Expression

Bacterial

Available Since

Aug. 23, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pET28a-Fgß-ddFLN4-HIS-ybbr

Plasmid

#101239

Purpose

wildtype Fgß peptide with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

Depositor

Hermann Gaub

Article

Milles et al Science. 2018 Mar 30;359(6383):1527-1533

Insert

Fgß peptide, ddFLN4 fingerprint (FGB Human, D. discoideum)

Tags

HIS and ybbr

Expression

Bacterial

Mutation

C18S in ddFLN4

Promoter

T7

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_P0526-sgRNAmreB

Plasmid

#149654

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, P0526

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b-HRASLS3-1-132

Plasmid

#100725

Purpose

Expression of N-terminal region of human HRASLS3 in E.coli

Depositor

Byung Il Lee

Article

Han et al Protein Expr Purif. 2010 May;71(1):103-7

Insert

HRASLS3 (PLAAT3 Human)

Tags

His

Expression

Bacterial

Promoter

T7 promotor

Available Since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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XOR gate-V2.0

Plasmid

#44453

Depositor

Drew Endy

Article

Bonnet et al Science. 2013 May 3;340(6132):599-603. d

Insert

XOR-gate

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 13, 2013

Availability

Academic Institutions and Nonprofits only

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GST cdc25c

Plasmid

#10969

Depositor

Toren Finkel

Article

Savitsky et al J Biol Chem. 2002 Jun 7. 277(23):20535-4

Insert

cdc25c (CDC25C Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
pET28a-SENP5 (catalytic domain)

Plasmid

#16358

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

human SENP5 catalytic domain (SENP5 Human)

Tags

His

Expression

Bacterial

Mutation

Amino Acid 567 to 755 catalytic domain of human S…

Available Since

Dec. 19, 2007

Availability

Academic Institutions and Nonprofits only
pUC57-codon-modified HTK

Plasmid

#34563

Depositor

David Anderson

Article

Lo et al Neuron. 2011 Dec 22;72(6):938-50.

Insert

codon-modified HTK

Expression

Bacterial

Mutation

codon modified HTK using mouse codon usage table

Promoter

pUC57 bacteria promoter

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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