30,735 results

1. pUltraI-Tet3.0[TAA]

   Plasmid

   #164580

   Purpose

   Machinery plasmid containing a single copy of the Mb Tet3.0RS and cognate tRNA for incorporation of Tet3.0 at TAA codons

   Depositor

   Ryan Mehl

   Article

   Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

   Inserts

   Mb Tet3.0RS (R2-3)
   Mm tRNA[TAA]
   

   Expression

   Bacterial

   Mutation

   L170G, N311G, C313A

   Promoter

   ProK and TacI

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET22-HT/plsA

   Plasmid

   #73625

   Purpose

   Produces Escherichia coli adenylate kinase (PlsA), N-terminal His6 tag

   Depositor

   James Williamson, T. J. Kappock

   Article

   Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

   Insert

   adenylate kinase (adk Escherichia coli str. K-12 substr. MG1655)
   

   Tags

   His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCOLA-T7-WspR

   Plasmid

   #79162

   Purpose

   Expresses WspR WT under a T7 promoter

   Depositor

   Ming Hammond

   Article

   Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

   Insert

   WspR (wspR P. areuginosa)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWSK29

   Plasmid

   #172972

   Purpose

   Low copy number cloning vector

   Depositor

   Renato Morona

   Article

   Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Aug. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMTTH+H1.4

   Plasmid

   #157796

   Purpose

   Expression of human linker histone H1.4

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   H1.4 (H1-4 Human)
   

   Tags

   His6 and MBP

   Expression

   Bacterial

   Promoter

   pTAC

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFC332

   Plasmid

   #87845

   Purpose

   AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker

   Depositor

   Uffe Mortensen

   Article

   Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

   Inserts

   Cas9
   hph (hygromycin resistance marker)
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Promoter

   Aspergillus nidulans tef1 promoter and Aspergillu…

   Available Since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCI-T7Max-UTR1-deGFP-8xHis-T500

   Plasmid

   #178422

   Purpose

   Expression of deGFP via a T7 Promoter in TXTL

   Depositor

   Kate Adamala

   Article

   Deich et al J Biol Eng. 2023 Jan 23;17(1):4. doi: 10

   Insert

   deGFP
   

   Use

   Synthetic Biology

   Tags

   8xHis Tag

   Expression

   Bacterial

   Promoter

   T7Max

   Available Since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-Tight-Puro_Nkx6.2

   Plasmid

   #64846

   Purpose

   A lentiviral vector for the expression of mouse Nkx6.2 under doxycyline control

   Depositor

   Paul Tesar

   Article

   Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

   Insert

   Nkx6.2 (Nkx6-2 Mouse)
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Available Since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNCS Citrine

   Plasmid

   #91761

   Purpose

   Bacterial expression of a fluorescent protein, Citrine

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

   Insert

   Citrine
   

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOlyA(WT)

    Plasmid

    #132391

    Purpose

    Ostreolysin A for sensing sphingomyelin/cholesterol complexes

    Depositor

    Arun Radhakrishnan

    Article

    Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

    Insert

    Ostreolysin A wild-type
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: C62S, C94S, S151C

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCgsgRNA_crtYf

    Plasmid

    #153338

    Purpose

    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; rep oriVC. glutamicum; pMB1 oriVE. Coli; Pj23119-crRNA targeting crtYf; Spcr

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

    Insert

    crRNA targeting crtYf
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET31b-T7-Spinach2-Ct

    Plasmid

    #79158

    Purpose

    Expresses the Ct biosensor under a T7 promoter

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Insert

    T7-tRNA-Spinach2-Ct
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOlyA(E69A)

    Plasmid

    #132392

    Purpose

    Mutant version of OlyA that senses sphingomyelin without or with cholesterol

    Depositor

    Arun Radhakrishnan

    Article

    Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

    Insert

    Ostreolysin A point mutant (E69A)
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: C62S, E69A, C94S, S151C

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVDL9.3

    Plasmid

    #168299

    Purpose

    Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

    Depositor

    Víctor de Lorenzo

    Article

    Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

    Insert

    Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
    

    Expression

    Bacterial

    Promoter

    LacI-Plac

    Available Since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. CpxR-GFP

    Plasmid

    #220150

    Purpose

    To track the natural CpxR promoter's transcriptional dynamics

    Depositor

    Andre Sanches Ribeiro

    Article

    Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

    Insert

    CpxR promoter only
    

    Expression

    Bacterial

    Available Since

    Aug. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKm

    Plasmid

    #170331

    Purpose

    backbone vector for clone construction

    Depositor

    Deqiang Duanmu

    Article

    Wang et al Front Microbiol. 2021 Jun 18;12:686008.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAurora

    Plasmid

    #190158

    Purpose

    Regulates bacterial expression of target gene in blue-light-activated manner. Based on Nakamurella multipartita PAL.

    Depositor

    Andreas Moeglich

    Article

    Ranzani et al ACS Synth Biol. 2022 Oct 21;11(10):3482-

    Type

    Empty backbone

    Tags

    His6 tag

    Expression

    Bacterial

    Available Since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET31b-T7-Spinach2-Dp

    Plasmid

    #79159

    Purpose

    Expresses the Dp biosensor under a T7 promoter

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Insert

    T7-tRNA-Spinach2-Dp
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. PJEBAN2

    Plasmid

    #203681

    Purpose

    Expresses Cyan Fluorescent Protein (CFP) in Listeria monocytogenes

    Depositor

    Tine Rask Licht

    Article

    Andersen et al BMC Microbiol. 2006 Oct 3;6:86. doi: 10.

    Insert

    Cyan Fluorescent Protein (CFP)
    

    Expression

    Bacterial

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTXTL-T7max-HiBiTGFPHis

    Plasmid

    #188317

    Purpose

    Expression of HiBiT-GFP via T7 promoter in TXTL

    Depositor

    Kate Adamala

    Article

    Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

    Insert

    HiBiT-GFP fusion
    

    Use

    Luciferase and Synthetic Biology

    Tags

    8x His-tag

    Expression

    Bacterial

    Promoter

    T7Max

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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