30,258 results

1. pFVK5

   Plasmid

   #167522

   Purpose

   Broad-Host range expression vector, RSF1010 origin, VanRAM repressor, PVanCC promoter, mKelly2, Gentamicin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   VanRAM-mKelly2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PVanCC

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSum-LacZ-gfp

   Plasmid

   #109285

   Purpose

   Expresses gfp in mycobacteria

   Depositor

   Nicolai van Oers

   Article

   Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   GFP modified for expression in prokaryotes; inclu…

   Promoter

   LacZalpha

   Available since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZP06B

   Plasmid

   #232321

   Purpose

   Make sacB a counterselection marker to replace galK

   Depositor

   Chenggang Wu

   Insert

   PrpsJ-sacB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCasAb-apr

   Plasmid

   #121998

   Purpose

   Bacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumannii

   Depositor

   Quanjiang Ji

   Article

   Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBad-EBFP2

   Plasmid

   #14891

   Purpose

   Bacterial expression of EBFP2 blue fluorescent protein

   Depositor

   Robert Campbell

   Article

   Ai et al Biochemistry. 2007 May 22;46(20):5904-10

   Has Service

   Cloning Grade DNA

   Insert

   EBFP2
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   The fluorescent protein was inserted between XhoI…

   Promoter

   Available since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBB542

   Plasmid

   #27395

   Depositor

   Bernd Bukau

   Article

   de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

   Insert

   dnaK, dnaJ, groESL
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHybrid-Electra2

   Plasmid

   #179475

   Purpose

   Expresses Electra2 in bacterial cells and Electra2 and mScarlet in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Inserts

   Electra2
   mScarlet-I
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKEW303-AcrVA5

   Plasmid

   #115657

   Purpose

   Expresses AcrVA5 in bacteria, under TetR control.

   Depositor

   Jennifer Doudna

   Article

   Watters et al Science. 2018 Oct 12;362(6411):236-239.

   Insert

   AcrVA5
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCB578

   Plasmid

   #141095

   Purpose

   E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array

   Depositor

   Chase Beisel

   Article

   Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

   Inserts

   Cas9 and tracrRNA
   repeat-spacer-repeat array
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. sgRNA_expression_vector

    Plasmid

    #210212

    Purpose

    An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.

    Depositor

    Albert Jeltsch

    Article

    Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-Expotin 1

    Plasmid

    #42047

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    Exportin-1 (XPO1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28 MBP_PrA/G-H6

    Plasmid

    #176077

    Purpose

    Express His6 tagged MBP_PrA/G in Escherichia coli

    Depositor

    Tom Rapoport

    Article

    Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

    Insert

    MBP PrA/G-H6
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-CjCas9

    Plasmid

    #89754

    Purpose

    Expression of CjCas9 with His tag in E.coli

    Depositor

    Seokjoong Kim

    Article

    Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

    Insert

    CjCas9
    

    Use

    CRISPR

    Tags

    6XHis, HA, and SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRE140

    Plasmid

    #118492

    Purpose

    broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    TagBFP2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAB151

    Plasmid

    #101666

    Purpose

    Opto-T7RNAP*(563-F1)

    Depositor

    Mustafa Khammash

    Article

    Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

    Insert

    Opto-T7RNAP*(563-F1)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCAS9counter

    Plasmid

    #107192

    Purpose

    Expresses CAS9 and sgRNA for targeted counterselection in S. aureus

    Depositor

    Steve Salipante

    Article

    Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

    Inserts

    Cas9
    sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    prab17

    Available since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)

    Plasmid

    #199604

    Purpose

    Expression of cell permeable spCas9 in E. Coli.

    Depositor

    Junwei Shi

    Article

    Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

    Insert

    Cas9-T6N
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCK302

    Plasmid

    #87768

    Purpose

    As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used

    Depositor

    John Heap

    Article

    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

    Inserts

    PrhaBAD-sfGFP
    rhaS from E. coli with its native RBS from E. coli
    

    Use

    Synthetic Biology

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaBAD rhamnose-inducible promoter from E. coli …

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-FLAG-Synaptojanin 1-170

    Plasmid

    #22292

    Depositor

    Pietro De Camilli

    Article

    Haffner et al FEBS Lett. 1997 Dec 15. 419(2-3):175-80.

    Insert

    Synaptojanin 1_170 (SYNJ1 Human)
    

    Use

    Tags

    Flag

    Expression

    Bacterial and Mammalian

    Mutation

    K334R compared to Genbank ID NM_003895

    Promoter

    Available since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21 gamma-tubulin

    Plasmid

    #101825

    Purpose

    production of gamma-tubulin in bacteria

    Depositor

    Ana Carrera, Maria Alvarado-Kristensson

    Article

    Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

    Insert

    gamma-tubulin 1 (TUBG1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Whole protein

    Promoter

    T7

    Available since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge