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pEM526

Plasmid

#248740

Purpose

Transcriptional reporter backbone/entry vector with mGL and constitutive mScarlet-I3 in pNBU2 in pNBU2 integrative Bacteroides plasmid

Depositor

Carolina Tropini

Article

McCallum et al Cell. 2026 Feb 19;189(4):1245-1261.e21.

Insert

PBfP1E6-RBS1PLP-mScarletI3-rrnBT2-[GG insertion site]

Expression

Bacterial

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pDSG310

Plasmid

#115611

Purpose

surface-displayed Ag2, Arabinose-inducible; medium-copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Expression

Bacterial

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pBT100.106b

Plasmid

#122601

Purpose

clone APOBEC1 phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

APOBEC1

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pSEVA-gRic6T

Plasmid

#106401

Purpose

E.coli-Pseudomonas putida KT2440 shuttle plasmid containing sgRNA targeting the KT2440 genome, and homologous arms.

Depositor

Sheng Yang

Article

Sun et al Microb Cell Fact. 2018 Mar 13;17(1):41.

Insert

sgRNA

Expression

Bacterial

Promoter

pj23119

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pET Biotin His6 mCherry LIC cloning vector (H6-mCherry)

Plasmid

#29722

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, AviTag (biotin), TEV cleavage site, and mC…

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pC013 - Twinstrep-SUMO-huLwCas13a

Plasmid

#90097

Purpose

Twinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

Insert

LwCas13a

Use

CRISPR

Tags

6xHis-Twin Strep-SUMO

Expression

Bacterial

Available Since

June 13, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pXY027

Plasmid

#98915

Purpose

Express FtsZ tagged with GFP in E coli

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

FtsZ

Tags

GFP

Expression

Bacterial

Promoter

T5-lac

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pKS1

Plasmid

#84079

Purpose

Expresses the non-toxic fragment C of tetanus toxin

Depositor

Neil Fairweather

Article

Sinha et al Mol Microbiol. 2000 Sep;37(5):1041-51.

Insert

Tetanus neurotoxin fragment C

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-luxOFP

Plasmid

#229945

Purpose

Bacterial expression of a de novo designed, FRET-based neoLux1.2-CyOFP1 fusion bioluminescent probe (max. Em=588)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxOFP

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pDonorCm(Ptr)

Plasmid

#175577

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

Mini-Tn, Cm cassette

Expression

Bacterial

Mutation

NO

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pKB225

Plasmid

#170015

Purpose

Encodes LacI-controlled expression of surface display protein with no C-terminal peptide and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
Peptide display protein with no C-terminal peptide

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Available Since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pEThT-CAHS1

Plasmid

#90027

Purpose

To express His-tagged CAHS1 in bacteria

Depositor

Takekazu Kunieda

Article

Yamaguchi et al PLoS One. 2012;7(8):e44209.

Insert

CAHS1

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET-32a(+)-TGFB3

Plasmid

#120288

Purpose

Produce human recombinant TGFB3 in DE3 cells

Depositor

Paul Burridge

Article

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

Transforming Growth Factor-β3 (TGFB3 Synthetic, Human)

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 promoter

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp

Plasmid

#183069

Purpose

Bacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c_4

Use

CRISPR

Tags

His10 and MBP

Expression

Bacterial

Mutation

Wild-type

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pFAB3677

Plasmid

#47833

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 2 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 2 and BCD1

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Dec. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pNBU2_erm-TetR-P1T_DP-GH023 - NanoLuc

Plasmid

#117728

Purpose

Integration vector at an attB site in Bacteroides; contains 1kb cassette of aTC-regulatable promoter (RBS GH023) upstream of the NanoLuc gene

Depositor

Andrew Goodman

Article

Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

Insert

NanoLuc

Use

Luciferase

Expression

Bacterial

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-OleD

Plasmid

#59372

Purpose

Inducible expression of OleD (DQ195536) under arabinose promoter

Depositor

Christopher Anderson

Insert

OleD-His

Expression

Bacterial

Promoter

pBAD

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pBS_nanorod_105b

Plasmid

#120347

Purpose

phagemid containing all components for assembly of a DNA origami nanorod (10-helix bundle)

Depositor

Hendrik Dietz

Article

Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

Insert

Staple sequences for a DNA origami nanorod

Expression

Bacterial

Promoter

none

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pSUM-Kan-MCS1-gfp

Plasmid

#109288

Purpose

Expresses high levels of gfp in mycobacteria

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

Multiple Cloning Site and GFP

Expression

Bacterial

Promoter

Kanamycin

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pQE80_T5_P3-ehaA_LacI

Plasmid

#186315

Purpose

Plasmid for inducible E coli expression of the P3 helixCAM.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Insert

P3-ehaA

Expression

Bacterial

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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