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pCDB179-TfCut2-S121P-D174S-D204P

Plasmid

#218695

Purpose

Encodes codon optimized TfCut2-S121P-D174S-D204P enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

TfCut2

Tags

10xHis

Expression

Bacterial

Mutation

S121P-D174S-D204P

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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hGAPDH-rLuc

Plasmid

#82479

Purpose

reporter of GAPDH expression

Depositor

Albee Messing

Article

Cho et al J Neurochem. 2009 Jul;110(1):343-51. Epu

Insert

GAPDH promoter (GAPDH Human)

Use

Luciferase

Expression

Bacterial

Promoter

hGAPDH

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pRSET-QNA

Plasmid

#129305

Purpose

Negative control for ATP measurement

Depositor

Hiroyuki Noji

Article

Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

Insert

QUEEN-NA

Expression

Bacterial

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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IVT-MCP-Phi29

Plasmid

#207456

Purpose

in vitro transcription of mRNA

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

Insert

MCP-Phi29

Expression

Bacterial

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-nsp7-nsp8

Plasmid

#159092

Purpose

Coexpression construct of Nsp7 with an N-terminal His-tag and Nsp8

Depositor

Seth Darst

Article

Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

Inserts

NSP7
NSP8

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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LOV-Turbo-His6_pET21a

Plasmid

#199668

Purpose

expresses LOV-Turbo in bacteria

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Tags

His6

Expression

Bacterial

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pSM019

Plasmid

#128291

Purpose

asymmetric plasmid partitioning E. coli - regulatory plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Para - lp-sfyfp-parB

Expression

Bacterial

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pWT019a

Plasmid

#107893

Purpose

W1.1 plasmid. Contains PTetO-sd2U-Cas9, PLacO-sgRNA1 and is part of CAMERA 1.1

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-sd2U-Cas9, PLacO-sgRNA1

Expression

Bacterial

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_mouse-Caspase-1_enzymatic-domain

Plasmid

#183385

Purpose

Overexpression of catalytic domain of inflammatory caspase in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-1 (AA130-404) (Casp1 Mouse)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX SH3(2)-4R

Plasmid

#112090

Purpose

Bacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3(2)-4R (NCK1 Human)

Tags

GST

Expression

Bacterial

Promoter

Lac

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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