34,239 results
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Plasmid#204847PurposeExpress His tagged-RGS4 in bacterial cells for protein purificationDepositorAvailable sinceSept. 14, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits
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pPath-Violacein-native
Plasmid#200001PurposeViolacein pathway. Native promoter and CDS sequenceDepositorInsertPathways
ExpressionBacterialAvailable sinceSept. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVSL10
Plasmid#201182PurposeBst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavableDepositorInsertBst polymerase large fragment (BF) F710Y/D598A
TagsHRV 3C protease cleavage site and His6ExpressionBacterialMutationF710Y D598APromoterT5-lacOAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.3TOPO-T7-hCas9
Plasmid#161876PurposeIn vitro transcription of SpCas9DepositorInserthuman codon optimized SpCas9
ExpressionBacterial and MammalianPromoterCMVAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MPRA_minCMV+GFP
Plasmid#204866PurposeVector containing GFP driven by minimal CMV promoter. This cassette can be cloned into MPRA vectors to serve as a reporter gene.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertminCMV:GFP
ExpressionBacterial and MammalianPromoterminimal CMVAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-YTHDF2
Plasmid#204406PurposeE. coli expression of full-length YTHDF2 with an N-terminal mCherry tagDepositorAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28C-mCherry-53BP1LCD
Plasmid#204407PurposeE. coli expression of the 53BP1 low-complexity domain (1208-1977) with an N-terminal mCherry tagDepositorAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-EGFP-NLS-3XFLAG-V5
Plasmid#196090PurposeExpresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGFP
TagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianPromoterCAGGsAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMGA-ptac-sfGFP
Plasmid#139934PurposeIPTG-inducible sfGFP expression on M. magneticum/E. coli shuttle vectorDepositorInsertLacI-Ptac-sfGFP
ExpressionBacterialAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIA034
Plasmid#200421PurposeamyE::(bsdronPA cm), for bsdronPA fusions. PALM integration vector includes codon optimized dronPA FP for B. subtilis. MCS for fast and interchangeable cloning.DepositorTypeEmpty backboneTagsbsdronPAExpressionBacterialAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMM7
Plasmid#201213PurposeIntegrative GFP-based shuttle vector for genetic engineering of Parageobacillus thermoglucosidasius. Carries homologous flanks for deletion of key sporulation regulator spo0A.DepositorInsertssfGFP
Spo0A right flank
Spo0A left flank
UseSynthetic BiologyExpressionBacterialMutationF11V, T13P, N39D, A179AAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCfuNca-Plastid
Plasmid#190818PurposePlastid targeting GFP with ble resistant gene for zeocinDepositorInsertsDiatom plastid targeted GFP
Bleomycin resistance gene
ExpressionBacterialPromoterCylindrotheca fusiformis fcp and Nitzschia captiv…Available sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBsVchCAST-pyrE
Plasmid#203813PurposeExpression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coliDepositorInsertVchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(pyrE)
UseCRISPRExpressionBacterialAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRM055 His-TEV-Cas9 6xNLS 0C
Plasmid#196246PurposeExpression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)DepositorInsertCas9
ExpressionBacterialPromoterT7Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJYP1
Plasmid#202406PurposeBackbone vector to clone toxic or unstable DNA using dual-control (transcriptional control via naringenin inducible pfdeA promoter - translational control via B12 riboswitch)DepositorTypeEmpty backboneExpressionBacterialPromoterfdeAAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCgVchDonor
Plasmid#203817PurposeDNA donor for transposition, shuttle vector for Corynebacterium glutamicum / E. coliDepositorInsertMini-Tn, Spec cassette
UseCRISPRExpressionBacterialAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
His-AgdNK
Plasmid#203226PurposeFor protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorInsertdeoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
TagsN-terminal TEV cleavable 6xHis tagExpressionBacterialAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMetTU1-A_Sv5K_araC-PBAD_MCD1_MegaB-S9G-R31L-R85L_MegaRNFGLSKJTU_Bba-B0015
Plasmid#202025PurposeExpresses Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant) in E. coliDepositorPublicationInsertBacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant)
ExpressionBacterialMutationGvpB S9G-R31L-R85LAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEXP5-NT/pLas eGFP
Plasmid#193630PurposeInducible expression of eGFP by the transcription factor complex LasR-3OC12 homoserine lactone (HSL).DepositorPublicationInserteGFP
Tags6xHisExpressionBacterialPromoterT7 RNAP promoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUCSh5vio-2300
Plasmid#170987PurposeEncodes sgRNA target 2300 and Gentamicin resistance - violacein pathway transposon.DepositorInsertSh-CAST sgRNA 2300 violacein
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterplacIAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only