32,115 results
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Plasmid#80423PurposeFor expression of EPN-01* in E. coliDepositorInsertEPN-01*
TagsMycExpressionBacterialAvailable SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
R26-CAG-rox-FNF-bpA-3pA-rox-td-sfGFP
Plasmid#180153Purposetd-sfGFP dre reporter with Rosa26 recombineering homology armsDepositorInserttd-sfGFP
UseMouse Targeting; Dre/roxExpressionBacterial and MammalianAvailable SinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUS252L
Plasmid#191832PurposeExpresses fuBFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuBFP to place in other plasmid backbonesDepositorInsertfuBFP
ExpressionBacterialAvailable SinceJan. 17, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
H6SUMO-IntC-P53(304-393)
Plasmid#200316Purposebacterial expression of P53 TET-CTD for segmental labelingDepositorInsertp53 (304-393) (TP53 Human)
TagsHis6-SUMO and designed intein CExpressionBacterialPromoterT7Available SinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tevP53(1-303)IntN
Plasmid#200312PurposeBacterial expression of P53 NTAD-DBD for segmental labelingDepositorInsertp53 (TP53 Human)
TagsHis6GB1tev and designed intein NExpressionBacterialMutationM133L, V203A, N239Y, N268DPromoterT7Available SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
gNS2B47NS3 L30SF31S
Plasmid#155316PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker L30SF31S mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
TagsHis tagExpressionBacterialMutationL30 to S, F31 to SPromoterM13Available SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-Nedd-4/2522
Plasmid#11466DepositorAvailable SinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
RAB14
Plasmid#25519PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceJan. 11, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMAL-c2x-MT3
Plasmid#32115DepositorAvailable SinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCas_5+7_gfp
Plasmid#60595Purposeencodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.DepositorInsertCas_5+7_gfp
ExpressionBacterialAvailable SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCas_2+5
Plasmid#60594Purposeencodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2DepositorInsertCas_2+5
ExpressionBacterialAvailable SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
GST-MAGE-2-429
Plasmid#89129Purposebacterial expression of full-length human MAGE-A11 with N-terminal GST tag for rapid isolation, in pGEX-4T-1 EcoRI and XhoI sitesDepositorAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKB230
Plasmid#170017PurposeEncodes LacI-controlled expression of surface-displayed protegrin-1 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Protegrin-1
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pgRNA-non-target
Plasmid#129465PurposeDerived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.DepositorInsertNon-target/random gRNA
UseCRISPRExpressionBacterialPromoterBBa_J23119 (SpeI)Available SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-G6
Plasmid#73437PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.DepositorInsertRepressor G6 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive wild-type S. pyogenes promoterAvailable SinceMarch 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
SBC015866
Plasmid#226280PurposeExpresses BsSfp, MsCAD, and SrCAR from trc promoter. Biosynthesis of (hydroxy)cinnamyl alcohols from (hydroxy)cinnamic acids.DepositorInserts4'-phosphopantetheinyl transferase Sfp
Cinnamyl alcohol dehydrogenase
Carboxylic acid reductase
ExpressionBacterialAvailable SinceNov. 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBK CUP1 EGFP
Plasmid#170210PurposeYeast two-hybrid vector using a copper inducible CUP1 promoter, expressing EGFPDepositorArticleInsertenhanced green fluorescent protein
TagsGAL4 DNA Binding Domain and MycExpressionBacterial and YeastPromoterCUP1Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19-MTdTc302
Plasmid#207458PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302DepositorInsertMTdTc302 (Dntt Mouse)
Tags10xHis-MBPExpressionBacterialMutationCys188Ala, Cys216Ser, Cys378Ala, and Cys438SerAvailable SinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3.5VPIII.EGFP.IGR2.1
Plasmid#40865DepositorAvailable SinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only