31,188 results
-
Plasmid#135942PurposeExpresses AUF1p45 in E. coliDepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
TagsFlag2 and His6ExpressionBacterialPromoterT7Available SinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKS014
Plasmid#65467PurposepSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) Ntag and beta-galactosidaseDepositorInsertNtag-beta galactosidase
UseSynthetic BiologyTagsFLAGExpressionBacterialPromoterpl-TetOAvailable SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
HAO1
Plasmid#38970PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
env8 selectivity (b')
Plasmid#99837PurposemNeonGreen reporter that places expression of a fluorescent protein reporter (mNeonGreen) under control of the env8HyCbl riboswitch.DepositorInsertenv8 selectivity (b')
TagsmNeonGreenExpressionBacterialMutationA20G, A68G, G-C pairs in P1 and P3Available SinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant
Plasmid#87350PurposeS867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET constructDepositorInsertCas9
TagsHis-MBPExpressionBacterialMutationC80S/S355C/C574S/S867CPromoterT7Available SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBEAST_J23101-bphC
Plasmid#128137PurposeThe cell-free adapted backbone, pBEAST, expressing bphC (the enzyme converting biphenyl-2,3-diol to 2-hydroxy-6-oxo-6-phenylhexa-2,4-) under control of the constitutive promoter J23101, and RBS B0032DepositorInsertbphC
UseSynthetic BiologyExpressionBacterialPromoterJ23101Available SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2207 pGEM p53
Plasmid#10853DepositorInsertp53 (TP53 Human)
ExpressionBacterialAvailable SinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-3xMyc
Plasmid#129569PurposeEmpty backbone for Myc TagDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBP-P_vanR-PVanCC
Plasmid#204078PurposeType 0 promoter partDepositorInsertvanR repressor and PVanCC vanillic acid-inducible promoter
ExpressionBacterialMutationNoneAvailable SinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAGE2.0-i
Plasmid#194639PurposeEmpty pAGE2.0 plasmid with pBAD inducible promoterDepositorTypeEmpty backboneUseSynthetic Biology; Diatom expressionExpressionBacterial and YeastAvailable SinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD/CURT_fluoA
Plasmid#117998PurposeExpression of CURT_fluoA controlled by the ARA promoterDepositorInsertCURT_fluoA
ExpressionBacterialPromoterARAAvailable SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
MP-S2
Plasmid#69691PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 AAG(Y127I-H136L)
ExpressionBacterialPromoterE. coli PBADAvailable SinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
MP-S3
Plasmid#69692PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 d80-AAG(Y127I-H136L)
ExpressionBacterialPromoterE. coli PBADAvailable SinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1G1
Plasmid#97325PurposeNanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.DepositorInsertMSP1D1G1
Tags6xHis - TEVExpressionBacterialMutationAddition of G residue after T25PromoterT7Available SinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
MP-P6
Plasmid#69665PurposeUntargetted Bacterial MutagenesisDepositorInsertdnaQ926 dam seqA emrR dinB
ExpressionBacterialPromoterE. coli PBADAvailable SinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
BRPF1B-c001
Plasmid#53620Purposebacterial expression plasmidDepositorInsertBRPF1B
TagsHis-6-TEVExpressionBacterialMutationBacterial expression for structure determination;…PromoterT7Available SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX Stat6-SH2
Plasmid#46517DepositorInsertStat6 (STAT6 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 510-654PromoterTacAvailable SinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
LB124 (Bcl-2 from -1287 to -3934)
Plasmid#15240DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterialMutationBcl-2 fragment from -1287 to -3934 with P1Available SinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
GST-Cav FL (1-178)
Plasmid#22445DepositorInsertCaveolin1(1-178)
TagsGSTExpressionBacterialAvailable SinceDec. 8, 2009AvailabilityAcademic Institutions and Nonprofits only -
pJMP1104
Plasmid#119248Purposeknockdown pqsC in P. aeruginosaDepositorInsertsgRNA pqsC (P. aeruginosa)
ExpressionBacterialMutationD10A and H840AAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only