33,128 results
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Plasmid#159415PurposeMedicago truncatula BCP1 promoter sequence (1108 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.DepositorInsertMtBCP1 Promoter
UsePuc19-derivedExpressionBacterialAvailable SinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
lox2272 Neo* lox2272
Plasmid#168769Purposelox2272 (lox variant)-flanked pPgk-pEM7-Neo*-bpA positive selection cassetteDepositorInsertNeo*
UseCre/Lox and Mouse TargetingExpressionBacterial and MammalianAvailable SinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pgRNAtet-phaZ
Plasmid#169874PurposeSingle guide RNA plasmid for Cas9 targeting phaZ in P. putidaDepositorInsertsgRNA towards phaZ in Pseudomonas putida
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-DmBelle_B
Plasmid#145963PurposeBacterial Expression of DmBelleDepositorAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB224
Plasmid#170014PurposeEncodes LacI-controlled expression of surface-displayed NC and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
NC
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCOLADuet-1_nsp10 (SARS-CoV-2)
Plasmid#169158PurposeTo express SARS-CoV-2 nsp10 in E. coliDepositorInsertnsp10 (ORF1ab SARS-CoV-2)
TagsMethionineExpressionBacterialMutationCodon optimised for E. coliPromoterT7Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBK CUP1 EGFP
Plasmid#170210PurposeYeast two-hybrid vector using a copper inducible CUP1 promoter, expressing EGFPDepositorArticleInsertenhanced green fluorescent protein
TagsGAL4 DNA Binding Domain and MycExpressionBacterial and YeastPromoterCUP1Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB233
Plasmid#170040PurposeEncodes PvirK-mNeonGreen reporterDepositorArticleInsertmNeonGreen
UseSynthetic BiologyExpressionBacterialPromoterPvirKAvailable SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-nb112
Plasmid#171702PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to a nanobody targeting the spike protein of SARS-CoV-2 via a flexible linker.DepositorInsertcsgBACEFG, with nanobody fused to CsgA
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221TrxA-AID-T7RNAP
Plasmid#167976PurposeExpresses fusion protein TrxA-AID-T7RNAP in E. coliDepositorInsertTrxA-AID-T7RNAP
TagsT7RNAP and TrxAExpressionBacterialPromoterPtetAvailable SinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221MBPc-AID-T7RNAP
Plasmid#167975PurposeExpresses fusion protein MBPc-AID-T7RNAP in E. coliDepositorInsertMBPc-AID-T7RNAP
TagsMBPc and T7RNAPExpressionBacterialPromoterTetR-PtetAvailable SinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNVLTv2-pvdIJD-Prha-base
Plasmid#169244PurposeSuicide vector for integrating expression cassette in P. putida (L-rhamnose induction)DepositorInsertrhaRS
ExpressionBacterialPromoterrhaSAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCas9-beta-mmr
Plasmid#169241PurposeCas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutationsDepositorInsertscas9
beta
mutL-E36K
UseCRISPR and Synthetic BiologyExpressionBacterialMutationChanged glutamic acid 36 to lysineAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMBPcyto-TEV-BseRI
Plasmid#157739Purposeempty vector adds TEV-protease-removable N-term His10 + maltose binding protein (without signal sequence); avoids unwanted residues in the final protein sequence by using the BseRI restriction sitesDepositorTypeEmpty backboneTagsHis10 + MBP (cytoplasmic expression; no signal se…ExpressionBacterialPromoterT7Available SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS32: pTwinStrep-SUMO-PmcTnsC
Plasmid#168165PurposePmcTnsC expression construct for protein purification.DepositorInsertPmcCAST TnsC
Tags6X His, Twin Strep and SUMOExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMS23: pHelper(PmcCAST)_entry_ΔTnsD
Plasmid#168156PurposeInducible expression of PmcCAST proteins (except TnsD). Two BsaI sites for spacer cloning.DepositorInsertsPmcCAST minimal CRISPR array
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB and TnsC)
PmcTniQ
ExpressionBacterialAvailable SinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFCiK5
Plasmid#167514PurposeBroad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, mKelly2, Gentamicin Resistance Marker.DepositorInsertCinRAM-mKelly2
UseSynthetic BiologyExpressionBacterialPromoterPCinAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFRR5
Plasmid#167506PurposeBroad-Host range expression vector, RSF1010 origin, RhaR-RhaS activator, PrhaBAD promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertRhaS-RhaR-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPrhaBADAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMLC-BB1
Plasmid#166922PurposeBackbone for assembly of multi-level gene expression controllersDepositorInsertCyOFP - Orange fluorescent protein - flanked by BsaI sites
UseSynthetic BiologyExpressionBacterialPromoterJ23100 constitutiveAvailable SinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTJV1Sc3-rpoB
Plasmid#166983PurposeGenerates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistanceDepositorAvailable SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only