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31,561 results

Showing: 1021 - 1040 of 31561 results

  1. pMG024 MBP-GSK3β-HA-His, GST_λPPase

    Plasmid
    #196182
    Purpose
    Co-expresses MBP-GSK3β-HA-His (human GSK3β as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β-HA-His
    Depositor
    Jesse Zalatan
    Article
    Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755
    Inserts
    GSK3B (GSK3B Human)
    λPPase
    Tags
    GST, HA, His6, and MBP
    Expression
    Bacterial
    Promoter
    Tac
    Available Since
    Jan. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    196182-plasmid-map-sequence-id-383259

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  10. pFLAG_attP

    Plasmid
    #110095
    Purpose
    Vector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Type
    Empty backbone
    Tags
    3xFLAG
    Expression
    Bacterial
    Available Since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110095-plasmid-map-sequence-id-317064

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  13. pCN-mBFP

    Plasmid
    #63847
    Purpose
    Encodes a NADPH dependent blue fluorescent protein expressed under a strong promoter and strong RBS without His-tagged.
    Depositor
    Howard Salis
    Article
    Ng et al Metab Eng. 2015 May;29:86-96. doi: 10.10
    Insert
    mBFP
    Expression
    Bacterial
    Mutation
    Promoter and RBS was swapped. His-tag was removed…
    Available Since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    63847-plasmid-map-sequence-id-106570

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  19. pCRISPomyces-2BD

    Plasmid
    #234428
    Purpose
    Plasmid for Streptomyces containing gene of modified Cas9-BD protein and sequence of sgRNA cloning template
    Depositor
    Min-Kyu Oh
    Article
    Kim et al Nat Commun. 2025 Jan 21;16(1):874. doi:
    Insert
    Modified Cas9 with polyaspartate linking
    Expression
    Bacterial
    Mutation
    Linked polyaspartate using glycine-serine linker
    Available Since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    234428-plasmid-map-sequence-id-468848

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  20. IBMc396

    Plasmid
    #161954
    Purpose
    Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHH
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T
    Expression
    Bacterial
    Promoter
    PBAD
    Available Since
    March 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    161954-plasmid-map-sequence-id-328230

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Showing: 1021 - 1040 of 31561 results